دورية أكاديمية
أعرض في EDS

Epigenetic Response of Yarrowia lipolytica to Stress: Tracking Methylation Level and Search for Methylation Patterns via Whole-Genome Sequencing.

التفاصيل البيبلوغرافية
العنوان: Epigenetic Response of Yarrowia lipolytica to Stress: Tracking Methylation Level and Search for Methylation Patterns via Whole-Genome Sequencing.
المؤلفون: Kubiak-Szymendera M; Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 460-637 Poznań, Poland., Pryszcz LP; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain., Białas W; Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 460-637 Poznań, Poland., Celińska E; Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 460-637 Poznań, Poland.
المصدر: Microorganisms [Microorganisms] 2021 Aug 24; Vol. 9 (9). Date of Electronic Publication: 2021 Aug 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: DNA methylation is a common, but not universal, epigenetic modification that plays an important role in multiple cellular processes. While definitely settled for numerous plant, mammalian, and bacterial species, the genome methylation in different fungal species, including widely studied and industrially-relevant yeast species, Yarrowia lipolytica , is still a matter of debate. In this paper, we report a differential DNA methylation level in the genome of Y. lipolytica subjected to sequential subculturing and to heat stress conditions. To this end, we adopted repeated batch bioreactor cultivations of Y. lipolytica subjected to thermal stress in specific time intervals. To analyze the variation in DNA methylation between stressed and control cultures, we (a) quantified the global DNA methylation status using an immuno-assay, and (b) studied DNA methylation patterns through whole-genome sequencing. Primarily, we demonstrated that 5 mC modification can be detected using a commercial immuno-assay, and that the modifications are present in Y. lipolytica 's genome at ~0.5% 5 mC frequency. On the other hand, we did not observe any changes in the epigenetic response of Y. lipolytica to heat shock (HS) treatment. Interestingly, we identified a general phenomenon of decreased 5 mC level in Y. lipolytica 's genome in the stationary phase of growth, when compared to a late-exponential epigenome. While this study provides an insight into the subculturing stress response and adaptation to the stress at epigenetic level by Y. lipolytica , it also leaves an open question of inability to detect any genomic DNA methylation level (either in CpG context or context-less) through whole-genome sequencing. The results of ONT sequencing, suggesting that 5 mC modification is either rare or non-existent in Y. lipolytica genome, are contradicted with the results of the immunoassay.
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معلومات مُعتمدة: DI2017 001047 Ministerstwo Edukacji i Nauki; 506.771.09.00 B Uniwersytet Przyrodniczy w Poznaniu; 754422 H2020 Marie Skłodowska-Curie Actions
فهرسة مساهمة: Keywords: Yarrowia lipolytica; epigenome; genomics; methylation level; next generation sequencing; stress response; yeast
تواريخ الأحداث: Date Created: 20210928 Latest Revision: 20211001
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8471669
DOI: 10.3390/microorganisms9091798
PMID: 34576693
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-2607
DOI:10.3390/microorganisms9091798