دورية أكاديمية
Development and Application of a Multiplex Real-Time Polymerase Chain Reaction Assay for the Simultaneous Detection of Bacterial Aetiologic Agents Associated With Equine Venereal Diseases.
| العنوان: | Development and Application of a Multiplex Real-Time Polymerase Chain Reaction Assay for the Simultaneous Detection of Bacterial Aetiologic Agents Associated With Equine Venereal Diseases. |
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| المؤلفون: | Kim SH; Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea; College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea., Lee JS; College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea., Lee JH; Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea., Kim YJ; Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea., Choi JG; Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea., Lee SK; Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea., Kim HJ; Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea., Yang SJ; Equine Hospital, Korea Racing Authority, Gyeonggi-do, Republic of Korea., Park T; Equine Hospital, Korea Racing Authority, Gyeonggi-do, Republic of Korea., Lee SK; Equine Hospital, Korea Racing Authority, Gyeonggi-do, Republic of Korea., Kang HE; Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea., Jeoung HY; Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea. Electronic address: jhy98@korea.kr., Park JY; Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea. |
| المصدر: | Journal of equine veterinary science [J Equine Vet Sci] 2021 Oct; Vol. 105, pp. 103721. Date of Electronic Publication: 2021 Jul 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 8216840 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0737-0806 (Print) Linking ISSN: 07370806 NLM ISO Abbreviation: J Equine Vet Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003- : New York : Elsevier Original Publication: [Wildomar, CA : William E. Jones, c1981- |
| مواضيع طبية MeSH: | Gram-Negative Bacterial Infections*/veterinary , Taylorella* , Taylorella equigenitalis*/genetics, Animals ; Horses ; Real-Time Polymerase Chain Reaction/veterinary |
| مستخلص: | Venereal diseases caused by bacteria are important to the equine industry due to economic losses caused by decline of conception rate in breeding horses. Therefore, identification of infected animals as well as the implementation of appropriate managerial procedures based on accurate diagnosis is critical. In this study, two types of multiplex real-time polymerase chain reaction with high sensitivity and specificity were developed for the simultaneous detection and differentiation of five commonly associated bacterial pathogens of venereal diseases in horses, consisting of Taylorella equigenitalis, Taylorella asinigenitalis, Pseudomonas aeruginosa, Klebsiella pneumoniae and Streptococcus zooepidemicus. The assay was applied to samples collected as part of the surveillance of T.equigenitalis infection in South Korea. Swab samples collected from horses in 2015 were tested. T. equigenitalis and K. pneumoniae was detected in 21 (21.0%) and two (2.0%) samples, respectively. No samples were positive for T. asinigenitalis, P. aeruginosa, and S. zooepidemicus. Application of this assay to an existing surveillance program has allowed for an enhanced surveillance for a wider range of venereal diseases of equine to be implemented in South Korea. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Contagious Equine Metritis; Klebsiella pneumonia; Multiplex real-time polymerase chain reaction; Pseudomonas aeruginosa; Taylorella equigenitalis |
| SCR Organism: | Taylorella asinigenitalis |
| تواريخ الأحداث: | Date Created: 20211005 Date Completed: 20211021 Latest Revision: 20211021 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jevs.2021.103721 |
| PMID: | 34607681 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0737-0806 |
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| DOI: | 10.1016/j.jevs.2021.103721 |