دورية أكاديمية
Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks.
| العنوان: | Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks. |
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| المؤلفون: | Lantoine J; Mechanobiology & Soft Matter Group, Interfaces and Complex Fluids Laboratory, Research Institute for Biosciences, University of Mons, 7000 Mons, Belgium., Procès A; Mechanobiology & Soft Matter Group, Interfaces and Complex Fluids Laboratory, Research Institute for Biosciences, University of Mons, 7000 Mons, Belgium.; Laboratory of Neuroscience, Research Institute for Biosciences, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, Belgium., Villers A; Laboratory of Neuroscience, Research Institute for Biosciences, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, Belgium., Halliez S; Univ. Lille, Inserm, CHU Lille, U1172 - LilNCog - Lille Neuroscience & Cognition, LabEx DISTALZ, F-59000 Lille, France., Buée L; Univ. Lille, Inserm, CHU Lille, U1172 - LilNCog - Lille Neuroscience & Cognition, LabEx DISTALZ, F-59000 Lille, France., Ris L; Laboratory of Neuroscience, Research Institute for Biosciences, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, Belgium., Gabriele S; Mechanobiology & Soft Matter Group, Interfaces and Complex Fluids Laboratory, Research Institute for Biosciences, University of Mons, 7000 Mons, Belgium. |
| المصدر: | ACS chemical neuroscience [ACS Chem Neurosci] 2021 Oct 20; Vol. 12 (20), pp. 3885-3897. Date of Electronic Publication: 2021 Oct 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101525337 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1948-7193 (Electronic) Linking ISSN: 19487193 NLM ISO Abbreviation: ACS Chem Neurosci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society |
| مواضيع طبية MeSH: | Astrocytes* , Receptors, Tumor Necrosis Factor, Type II*, Cells, Cultured ; Cytokines ; Humans ; Tumor Necrosis Factor-alpha |
| مستخلص: | Deformation, compression, or stretching of brain tissues cause diffuse axonal injury (DAI) and induce structural and functional alterations of astrocytes, the most abundant cell type in the brain. To gain further insight into the role of mechanically activated astrocytes on neuronal networks, this study was designed to investigate whether cytokines released by mechanically activated astrocytes can affect the growth and synaptic connections of cortical neuronal networks. Astrocytes were cultivated on elastic membranes and subjected to repetitive mechanical insults, whereas well-defined protein micropatterns were used to form standardized neuronal networks. GFAP staining showed that astrocytes were mechanically activated after two cycles of stretch and mesoscale discovery assays indicated that injured astrocytes released four major cytokines. To understand the role of these cytokines, neuronal networks were cultured with the supernatant of healthy or mechanically activated astrocytes, and the individual contribution of the proinflammatory cytokine tumor necrosis factor-α (TNF-α) was studied. We found that the supernatant of two-cycle stretched astrocytes decreased presynaptic terminals and indicated that TNF-α must be considered a key player of the synaptic loss. Furthermore, our results indicate that cytokines released by injured astrocytes significantly modulate the balance between TNFR1 and TNFR2 receptors by enhancing R2 receptors. We demonstrated that TNF-α is not involved in this process, suggesting a predominant role of other secreted cytokines. Together, these results contribute to a better understanding of the consequences of repetitive astrocyte deformations and highlight the role of inflammatory signaling pathways in synaptic plasticity and modulation of TNFR1 and TNFR2 receptors. |
| فهرسة مساهمة: | Keywords: astrocytes; cytokines; mechanical injury; neuronal network |
| المشرفين على المادة: | 0 (Cytokines) 0 (Receptors, Tumor Necrosis Factor, Type II) 0 (Tumor Necrosis Factor-alpha) |
| تواريخ الأحداث: | Date Created: 20211006 Date Completed: 20211028 Latest Revision: 20220531 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1021/acschemneuro.1c00488 |
| PMID: | 34614352 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1948-7193 |
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| DOI: | 10.1021/acschemneuro.1c00488 |