دورية أكاديمية
In silico analyses and design of a chimeric protein containing epitopes of SpaC, PknG, NanH, and SodC proteins for the control of caseous lymphadenitis.
| العنوان: | In silico analyses and design of a chimeric protein containing epitopes of SpaC, PknG, NanH, and SodC proteins for the control of caseous lymphadenitis. |
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| المؤلفون: | Silva MTO; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Prédio 19, Pelotas, RS, 96010-900, Brazil., de Pinho RB; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Prédio 19, Pelotas, RS, 96010-900, Brazil., Bezerra FSB; Laboratório de Biotecnologia Aplicada a Doenças Infecto-Parasitárias, Centro de Ciências Biológicas e da Saúde, UFERSA, Mossoró, RN, Brazil., Scholl NR; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Prédio 19, Pelotas, RS, 96010-900, Brazil., Moron LD; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Prédio 19, Pelotas, RS, 96010-900, Brazil., Alves MSD; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Prédio 19, Pelotas, RS, 96010-900, Brazil., Woloski RDS; Laboratório de Bioinformática e Proteômica, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, RS, 96010-900, Brazil., Kremer FS; Laboratório de Bioinformática e Proteômica, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, RS, 96010-900, Brazil., Borsuk S; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Prédio 19, Pelotas, RS, 96010-900, Brazil. sibeleborsuk@gmail.com. |
| المصدر: | Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2021 Nov; Vol. 105 (21-22), pp. 8277-8286. Date of Electronic Publication: 2021 Oct 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer International, c1984- |
| مواضيع طبية MeSH: | Corynebacterium Infections* , Lymphadenitis*, Animals ; Computer Simulation ; Epitopes/genetics ; Goats ; Mice ; Recombinant Fusion Proteins/genetics ; Sheep |
| مستخلص: | Caseous lymphadenitis (CLA) is an infectious disease that affects goats and sheep causing drastic impacts on milk and meat production and is caused by Corynebacterium pseudotuberculosis. The disease can be prevented through vaccination but currently, vaccines demonstrate limited efficacy consequently leading to a need for the development of new ones. Here, we described the in silico development of a new chimeric protein constructed with epitopes identified from the sequences of the genes nanH, pknG, spaC, and sodC, previously described as potential vaccinal targets against C. pseudotuberculosis. The chimera was expressed, purified, and its immunogenicity was evaluated using sera of immunized mice. Results indicate the chimeric protein was able to stimulate antibody production. Additionally, analysis using serum from naturally infected goats showed that the protein is recognized by sera from these animals, indicating the possibility for using this chimera in new diagnostic methods. KEY POINTS: • The chimera was expressed with 52 kDa and a yield of 7 mg/L after purification. • The chimera was recognized by the sera of animals immunized with this formulation. • Chimera reacted with the serum of goats naturally infected with C. pseudotuberculosis. (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: C. pseudotuberculosis; Chimera; Epitopes; Immunodominant; Immunoinformatics |
| المشرفين على المادة: | 0 (Epitopes) 0 (Recombinant Fusion Proteins) |
| تواريخ الأحداث: | Date Created: 20211008 Date Completed: 20211102 Latest Revision: 20211102 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8497191 |
| DOI: | 10.1007/s00253-021-11619-x |
| PMID: | 34622335 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1432-0614 |
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| DOI: | 10.1007/s00253-021-11619-x |