دورية أكاديمية
COVID-19 cynomolgus macaque model reflecting human COVID-19 pathological conditions.
| العنوان: | COVID-19 cynomolgus macaque model reflecting human COVID-19 pathological conditions. |
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| المؤلفون: | Urano E; Laboratory of Immunoregulation and Vaccine Research, Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Tsukuba 305-0843, Japan., Okamura T; Laboratory of Immunoregulation and Vaccine Research, Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Tsukuba 305-0843, Japan., Ono C; Laboratory of Virus Control, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan., Ueno S; Department of Infectious Diseases and Host Defense, Graduate School of Medicine, Gunma University, Maebashi 371-8511, Japan., Nagata S; Laboratory of Antibody Design, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan., Kamada H; Laboratory of Biopharmaceutical Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan., Higuchi M; Laboratory of Immunoregulation and Vaccine Research, Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Tsukuba 305-0843, Japan., Furukawa M; Laboratory of Immunoregulation and Vaccine Research, Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Tsukuba 305-0843, Japan., Kamitani W; Department of Infectious Diseases and Host Defense, Graduate School of Medicine, Gunma University, Maebashi 371-8511, Japan., Matsuura Y; Laboratory of Virus Control, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan., Kawaoka Y; Division of Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706.; Department of Special Pathogens, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan., Yasutomi Y; Laboratory of Immunoregulation and Vaccine Research, Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Tsukuba 305-0843, Japan; yasutomi@nibiohn.go.jp.; Division of Immunoregulation, Department of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Mie 514-8507, Japan. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Oct 26; Vol. 118 (43). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Disease Models, Animal*, COVID-19/*immunology , Macaca fascicularis/*immunology , Primate Diseases/*immunology , SARS-CoV-2/*immunology, Animals ; Antibodies, Viral/blood ; Antibodies, Viral/immunology ; COVID-19/virology ; Female ; Humans ; Immunoglobulin G/blood ; Immunoglobulin G/immunology ; Lung/diagnostic imaging ; Lung/immunology ; Lung/virology ; Macaca fascicularis/virology ; Male ; Primate Diseases/virology ; SARS-CoV-2/physiology ; Tomography, X-Ray Computed/methods ; Virus Shedding/immunology ; Virus Shedding/physiology |
| مستخلص: | The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global threat to human health and life. A useful pathological animal model accurately reflecting human pathology is needed to overcome the COVID-19 crisis. In the present study, COVID-19 cynomolgus monkey models including monkeys with underlying diseases causing severe pathogenicity such as metabolic disease and elderly monkeys were examined. Cynomolgus macaques with various clinical conditions were intranasally and/or intratracheally inoculated with SARS-CoV-2. Infection with SARS-CoV-2 was found in mucosal swab samples, and a higher level and longer period of viral RNA was detected in elderly monkeys than in young monkeys. Pneumonia was confirmed in all of the monkeys by computed tomography images. When monkeys were readministrated SARS-CoV-2 at 56 d or later after initial infection all of the animals showed inflammatory responses without virus detection in swab samples. Surprisingly, in elderly monkeys reinfection showed transient severe pneumonia with increased levels of various serum cytokines and chemokines compared with those in primary infection. The results of this study indicated that the COVID-19 cynomolgus monkey model reflects the pathophysiology of humans and would be useful for elucidating the pathophysiology and developing therapeutic agents and vaccines. Competing Interests: The authors declare no competing interest. (Copyright © 2021 the Author(s). Published by PNAS.) |
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| فهرسة مساهمة: | Keywords: COVID-19; SARS-CoV-2; elderly; nonhuman primate; underlying disease |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Immunoglobulin G) |
| تواريخ الأحداث: | Date Created: 20211009 Date Completed: 20211025 Latest Revision: 20240819 |
| رمز التحديث: | 20240819 |
| مُعرف محوري في PubMed: | PMC8639365 |
| DOI: | 10.1073/pnas.2104847118 |
| PMID: | 34625475 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
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| DOI: | 10.1073/pnas.2104847118 |