دورية أكاديمية
Prevalence and Molecular Identification of Trypanosomes in Dogs in Enugu North Senatorial Zone, South East Nigeria.
| العنوان: | Prevalence and Molecular Identification of Trypanosomes in Dogs in Enugu North Senatorial Zone, South East Nigeria. |
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| المؤلفون: | Obi CF; Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria. Chukwunonso.obi@unn.edu.ng., Ezeh IO; Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria., Okpala MI; Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria., Agina O; Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria., Umeakuana PU; Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria., Essuman GAA; West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana.; Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana., Gwira TM; West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana.; Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana., Ezeokonkwo RC; Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria. |
| المصدر: | Acta parasitologica [Acta Parasitol] 2022 Mar; Vol. 67 (1), pp. 391-402. Date of Electronic Publication: 2021 Oct 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 9301947 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1896-1851 (Electronic) Linking ISSN: 12302821 NLM ISO Abbreviation: Acta Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Cham : Springer International Publishing Original Publication: Warszawa : Witold Stefanski Institute Of Parasitology |
| مواضيع طبية MeSH: | Trypanosoma*/genetics , Trypanosomiasis, African*/epidemiology, Animals ; Cross-Sectional Studies ; Dogs ; Nigeria/epidemiology ; Prevalence |
| مستخلص: | Purpose: Dogs are of immense social, psychological and economic importance in Nigeria and are severely affected by African trypanosomosis. However, the prevalence of canine African trypanosomosis (CAT) in Nigeria is underreported and the identification of the parasites relies mostly on basic morphological characteristics under the microscope, which could be misleading. The present study was carried out to determine the prevalence and characterize trypanosomes isolated from dogs in South east Nigeria. Methods: A cross-sectional survey was carried out to determine the prevalence and molecular identification of trypanosomes in dogs in Enugu North Senatorial Zone (ENSZ), South east Nigeria. Dogs (n = 450) were randomly sampled, their blood collected and some characteristics such as sex, breed, sampling location, season and age duly noted. The blood samples were screened for trypanosomosis using standard trypanosome detection techniques. Trypanosome-positive blood samples were spotted on FTA® cards for molecular identification using nested Tubulin-PCR, ITS-PCR, TgsGP-PCR, and DNA sequencing. Some hematological parameters of the dogs such as packed cell volume (PCV), total leucocyte count (TLC), red blood cell count (RBC) were also determined. Results: Of the 450 dogs sampled, 51 dogs were positive for trypanosomes with a prevalence rate of 11.3% (95% CI = 0.087-0.146). Trypanosoma brucei was the predominant trypanosome species infecting dogs in the study area. T. congolense, T. evansi, and T. vivax were also identified. The prevalence of canine trypanosomosis was significantly associated with season (χ 2 = 13.821, df = 1, P = 0.0001) and the sampling location (χ 2 = 6.900, df = 2, P = 0.032) while sex, breed, and age were not. The PCV and RBC of the infected dogs were significantly lower (p < 0.0001) than those of the uninfected dogs. Conclusions: CAT due to T. brucei is very prevalent in Enugu North Senatorial Zone, South east Nigeria and is associated with hematological changes. Our study also detected T. vivax in dogs in South east Nigeria which appears to be the first report of T. vivax in a dog in Nigeria. (© 2021. Witold Stefański Institute of Parasitology, Polish Academy of Sciences.) |
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| معلومات مُعتمدة: | TETFUND/DESS/UNI/NSUKKA/2018/RP/VOL.1 tertiary education trust fund; ACE02-WACCBIP: Awandare world bank group |
| فهرسة مساهمة: | Keywords: Canine African trypanosomosis; Dogs; Molecular identification; Prevalence; South east Nigeria; Trypanosomes |
| تواريخ الأحداث: | Date Created: 20211009 Date Completed: 20220323 Latest Revision: 20220323 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/s11686-021-00475-4 |
| PMID: | 34625859 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1896-1851 |
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| DOI: | 10.1007/s11686-021-00475-4 |