دورية أكاديمية
أعرض في EDS

Fatty acids can inhibit Staphylococcus aureus SaeS activity at the membrane independent of alterations in respiration.

التفاصيل البيبلوغرافية
العنوان: Fatty acids can inhibit Staphylococcus aureus SaeS activity at the membrane independent of alterations in respiration.
المؤلفون: DeMars ZR; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, USA., Krute CN; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, USA., Ridder MJ; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, USA., Gilchrist AK; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, USA., Menjivar C; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, USA., Bose JL; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, USA.
المصدر: Molecular microbiology [Mol Microbiol] 2021 Nov; Vol. 116 (5), pp. 1378-1391. Date of Electronic Publication: 2021 Oct 30.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8712028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2958 (Electronic) Linking ISSN: 0950382X NLM ISO Abbreviation: Mol Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, OX ; Boston, MA : Blackwell Scientific Publications, c1987-
مواضيع طبية MeSH: Bacterial Proteins/*drug effects , Bacterial Proteins/*metabolism , Fatty Acids/*pharmacology , Protein Kinases/*drug effects , Protein Kinases/*metabolism , Staphylococcus aureus/*metabolism , Transcription Factors/*drug effects , Transcription Factors/*metabolism, Gene Expression Regulation, Bacterial ; Humans ; Respiration ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/enzymology ; Staphylococcus aureus/pathogenicity ; Virulence
مستخلص: In Staphylococcus aureus, the two-component system SaeRS is responsible for regulating various virulence factors essential for the success of this pathogen. SaeRS can be stimulated by neutrophil-derived products but has also recently been shown to be inactivated by the presence of free fatty acids. A mechanism for how fatty acids negatively impacts SaeRS has not been described. We found that unsaturated fatty acids, as well as fatty acids not commonly found in Staphylococcal membranes, prevent the activation of SaeRS at a lower concentration than their saturated counterparts. These fatty acids can negatively impact SaeRS without altering the respiratory capacity of the bacterium. To uncover a potential mechanism for how fatty acids impact SaeRS function/activity, we utilized a naturally occurring point mutation found in S. aureus as well as chimeric SaeS proteins. Using these tools, we identified that the native transmembrane domains of SaeS dictate the transcriptional response to fatty acids in S. aureus. Our data support a model where free fatty acids alter the activity of the two-component system SaeRS directly through the sensor kinase SaeS and is dependent on the transmembrane domains of the protein.
(© 2021 John Wiley & Sons Ltd.)
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معلومات مُعتمدة: R01 AI121073 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: S. aureus; MRSA; SaeRS; two-component system; virulence factor
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Fatty Acids)
0 (SaeR protein, Staphylococcus aureus)
0 (Transcription Factors)
EC 2.7.- (Protein Kinases)
EC 2.7.3.- (SaeS protein, Staphylococcus aureus)
تواريخ الأحداث: Date Created: 20211009 Date Completed: 20220125 Latest Revision: 20221102
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8898382
DOI: 10.1111/mmi.14830
PMID: 34626146
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-2958
DOI:10.1111/mmi.14830