دورية أكاديمية
Live attenuated Leishmania major p27 gene knockout as a novel vaccine candidate: A study on safety, protective immunity and efficacy against canine leishmaniasis caused by Leishmania infantum.
| العنوان: | Live attenuated Leishmania major p27 gene knockout as a novel vaccine candidate: A study on safety, protective immunity and efficacy against canine leishmaniasis caused by Leishmania infantum. |
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| المؤلفون: | Elikaee S; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Pacific University School of Pharmacy, Hillsboro, Oregon, USA., Zarei Z; Meshkin Shahr Station, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran., Khamesipour A; Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran., Akhoundi B; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Borjian AR; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Afshar MJA; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Allely QK; School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Heidari S; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Mohebali M; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: mohebali@tums.ac.ir. |
| المصدر: | Acta tropica [Acta Trop] 2022 Jan; Vol. 225, pp. 106153. Date of Electronic Publication: 2021 Oct 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Basel. |
| مواضيع طبية MeSH: | Dog Diseases*/prevention & control , Leishmania infantum*/genetics , Leishmania major*/genetics , Leishmaniasis* , Leishmaniasis Vaccines* , Leishmaniasis, Visceral*/prevention & control , Leishmaniasis, Visceral*/veterinary, Animals ; Dogs ; Female ; Gene Knockout Techniques ; Mice ; Mice, Inbred BALB C |
| مستخلص: | Canine leishmaniasis (CanL) is an important parasitic e disease caused by Leishmania infantum and is transmitted by female phlebotomine sand flies primarily between canines and secondarily to humans. Recently, we showed that immunization with Leishmania major p27 gene knockout (Lmp27 -/- ) as a live attenuated vaccine was safe, induced immunogenicity, and protected against the development cutaneous and visceral leishmaniasis in mice. The p27 protein is a component of the COX protein complex which is responsible for ATP production. In this study, we analyzed the Lmp27 -/- candidate vaccine potential with this regard to the safety and induction of immunogenicity and protection against CanL. Variables such a clinical manifestation, anti-Leishmania antibodies using direct agglutination test (DAT), lymphocyte proliferation, delayed-type hypersensitivity (DTH), bone marrow aspiration (BMA) and parasite burden using parasitological and molecular examinations were measured. The results demonstrated that the Lmp27 -/- vaccinated group showed no clinical signs after inoculation with Lmp27 -/- mutant during a 12-month follow-up, and had significantly higher T-cell responses (Lymphocyte proliferation and DTH), lower seroconversion and parasite burdens following a challenge inoculation with L. infantum after 6-mounth. In conclusion, vaccination with Lmp27 -/- parasites would be safe and provide significant immunoprotectivity and efficacy against infection with wild type (WT) L. infantum. (Copyright © 2021 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Immunogenicity; Leishmania infantum; Leishmania major; Protection; dog; p27 gene knockout |
| المشرفين على المادة: | 0 (Leishmaniasis Vaccines) |
| تواريخ الأحداث: | Date Created: 20211011 Date Completed: 20211129 Latest Revision: 20211129 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.actatropica.2021.106153 |
| PMID: | 34634264 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6254 |
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| DOI: | 10.1016/j.actatropica.2021.106153 |