دورية أكاديمية
أعرض في EDS

Zinc Finger Protein ZFP36L1 Inhibits Flavivirus Infection by both 5'-3' XRN1 and 3'-5' RNA-Exosome RNA Decay Pathways.

التفاصيل البيبلوغرافية
العنوان: Zinc Finger Protein ZFP36L1 Inhibits Flavivirus Infection by both 5'-3' XRN1 and 3'-5' RNA-Exosome RNA Decay Pathways.
المؤلفون: Chiu H; Graduate Institute of Microbiology, National Taiwan University, Taipei, Taiwan.; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan., Chiu HP; Graduate Institute of Microbiology, National Taiwan University, Taipei, Taiwan.; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan., Yu HP; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan., Lin LH; National Mosquito-Borne Diseases Control Research Center, National Health Research Institute, Taipei, Taiwan., Chen ZP; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan., Lin YL; Graduate Institute of Microbiology, National Taiwan University, Taipei, Taiwan.; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.; Genomics Research Center, Academia Sinica, Taipei, Taiwan., Lin RJ; National Mosquito-Borne Diseases Control Research Center, National Health Research Institute, Taipei, Taiwan.; Ph.D. Program in Medical Biotechnology, Taipei Medical University, Taipei, Taiwan.
المصدر: Journal of virology [J Virol] 2022 Jan 12; Vol. 96 (1), pp. e0166521. Date of Electronic Publication: 2021 Oct 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE
أسماء مطبوعة: Publication: Washington Dc : American Society For Microbiology
Original Publication: Baltimore, American Society for Microbiology.
مواضيع طبية MeSH: RNA Stability* , Virus Replication*, Butyrate Response Factor 1/*metabolism , Exoribonucleases/*metabolism , Exosome Multienzyme Ribonuclease Complex/*metabolism , Flavivirus/*physiology , Flavivirus Infections/*metabolism , Flavivirus Infections/*virology , Microtubule-Associated Proteins/*metabolism, 3' Untranslated Regions ; Amino Acid Motifs ; Butyrate Response Factor 1/chemistry ; Dengue Virus/physiology ; Encephalitis Virus, Japanese/physiology ; Host-Pathogen Interactions ; Humans ; Protein Binding ; Protein Interaction Domains and Motifs ; RNA, Viral/genetics ; RNA, Viral/metabolism ; RNA-Binding Proteins
مستخلص: Zinc-finger protein 36, CCCH type-like 1 (ZFP36L1), containing tandem CCCH-type zinc-finger motifs with an RNA-binding property, plays an important role in cellular RNA metabolism mainly by RNA decay pathways. Recently, we demonstrated that human ZFP36L1 has potent antiviral activity against influenza A virus infection. However, its role in the host defense response against flaviviruses has not been addressed. Here, we demonstrate that ZFP36L1 functions as a host innate defender against flaviviruses, including Japanese encephalitis virus (JEV) and dengue virus (DENV). Overexpression of ZFP36L1 reduced JEV and DENV infection, and ZFP36L1 knockdown enhanced viral replication. ZFP36L1 destabilized the JEV genome by targeting and degrading viral RNA mediated by both 5'-3' XRN1 and 3'-5' RNA-exosome RNA decay pathways. Mutation in both zinc-finger motifs of ZFP36L1 disrupted RNA-binding and antiviral activity. Furthermore, the viral RNA sequences specifically recognized by ZFP36L1 were mapped to the 3'-untranslated region of the JEV genome with the AU-rich element (AUUUA) motif. We extend the function of ZFP36L1 to host antiviral defense by directly binding and destabilizing the viral genome via recruiting cellular mRNA decay machineries. IMPORTANCE Cellular RNA-binding proteins are among the first lines of defense against various viruses, particularly RNA viruses. ZFP36L1 belongs to the CCCH-type zinc-finger protein family and has RNA-binding activity; it has been reported to bind directly to the AU-rich elements (AREs) of a subset of cellular mRNAs and then lead to mRNA decay by recruiting mRNA-degrading enzymes. However, the antiviral potential of ZFP36L1 against flaviviruses has not yet been fully demonstrated. Here, we reveal the antiviral potential of human ZFP36L1 against Japanese encephalitis virus (JEV) and dengue virus (DENV). ZFP36L1 specifically targeted the ARE motif within viral RNA and triggered the degradation of viral RNA transcripts via cellular degrading enzymes 5'-3' XRN1 and 3'-5' RNA exosome. These findings provide mechanistic insights into how human ZFP36L1 serves as a host antiviral factor to restrict flavivirus replication.
References: J Virol. 2004 Dec;78(23):12781-7. (PMID: 15542630)
Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):151-6. (PMID: 17185417)
J Cell Biochem. 2007 Apr 15;100(6):1477-92. (PMID: 17133347)
Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):24303-24309. (PMID: 31719195)
Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3392-401. (PMID: 26056259)
Proc Jpn Acad Ser B Phys Biol Sci. 2018;94(6):248-258. (PMID: 29887569)
Eur J Cell Biol. 2016 Aug;95(8):277-84. (PMID: 27182009)
Science. 2016 Apr 22;352(6284):453-9. (PMID: 27102483)
Sci Rep. 2018 May 9;8(1):7381. (PMID: 29743536)
Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2681-2686. (PMID: 28213497)
Int Immunol. 2017 Apr 1;29(4):149-155. (PMID: 28369485)
J Immunol. 2014 Oct 15;193(8):4159-68. (PMID: 25225661)
J Virol. 2003 Nov;77(21):11555-62. (PMID: 14557641)
Cell Host Microbe. 2009 Apr 23;5(4):318-28. (PMID: 19380111)
Vaccine. 2009 May 11;27(21):2746-54. (PMID: 19366580)
J Clin Invest. 2017 Oct 2;127(10):3741-3754. (PMID: 28891815)
J Virol. 2011 May;85(10):4698-706. (PMID: 21367899)
J Virol. 2014 Feb;88(4):2116-30. (PMID: 24335297)
J Infect Dis. 2004 Nov 1;190(9):1618-26. (PMID: 15478067)
J Immunol. 2009 Dec 15;183(12):8035-43. (PMID: 19923450)
J Biol Chem. 2002 Dec 13;277(50):48558-64. (PMID: 12324455)
Dev Dyn. 2006 Nov;235(11):3144-55. (PMID: 17013884)
J Neurovirol. 2003 Apr;9(2):274-83. (PMID: 12707858)
Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):19083-8. (PMID: 24191027)
Nature. 2017 Oct 5;550(7674):124-127. (PMID: 28953888)
Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15834-9. (PMID: 21876179)
Nat Immunol. 2018 Feb;19(2):120-129. (PMID: 29348497)
Skelet Muscle. 2018 Dec 7;8(1):37. (PMID: 30526691)
Wiley Interdiscip Rev RNA. 2011 Jan-Feb;2(1):42-57. (PMID: 21278925)
Tissue Eng. 2006 Jul;12(7):1741-51. (PMID: 16889505)
Nat Commun. 2019 Jan 8;10(1):77. (PMID: 30622281)
Nat Immunol. 2010 Aug;11(8):717-24. (PMID: 20622884)
Mol Cell Biol. 1995 Apr;15(4):2219-30. (PMID: 7891716)
PLoS One. 2012;7(11):e49841. (PMID: 23185455)
J Virol. 2014 Jun;88(12):6793-804. (PMID: 24696471)
EMBO J. 2002 Sep 2;21(17):4709-18. (PMID: 12198173)
Biochemistry. 2003 Jan 14;42(1):217-21. (PMID: 12515557)
Biotechniques. 2000 Nov;29(5):970-2. (PMID: 11084856)
PLoS One. 2014 Jul 11;9(7):e102625. (PMID: 25014217)
Nucleic Acids Res. 2013 Mar 1;41(5):3314-26. (PMID: 23355615)
Microbes Infect. 2006 Sep;8(11):2647-56. (PMID: 16935542)
Virus Res. 2015 Aug 3;206:108-19. (PMID: 25683510)
Genes Dev. 2005 Feb 1;19(3):351-61. (PMID: 15687258)
Nat Rev Immunol. 2017 Feb;17(2):130-143. (PMID: 27990022)
Nat Cell Biol. 2015 Sep;17(9):1205-17. (PMID: 26280535)
J Biomed Biotechnol. 2009;2009:634520. (PMID: 19672455)
J Biol Chem. 2002 Mar 15;277(11):9606-13. (PMID: 11782475)
Protein Cell. 2010 Aug;1(8):752-9. (PMID: 21203916)
Science. 2002 Sep 6;297(5587):1703-6. (PMID: 12215647)
Protein Sci. 2010 Jun;19(6):1222-34. (PMID: 20506496)
Nat Struct Mol Biol. 2004 Mar;11(3):257-64. (PMID: 14981510)
J Virol. 2007 Mar;81(5):2391-400. (PMID: 17182693)
Mol Cell Biol. 2004 Jul;24(14):6445-55. (PMID: 15226444)
J Med Virol. 1997 Feb;51(2):132-6. (PMID: 9021544)
J Biol Chem. 2000 Jun 9;275(23):17827-37. (PMID: 10751406)
Trends Microbiol. 2002 Feb;10(2):100-3. (PMID: 11827812)
PLoS One. 2012;7(11):e50387. (PMID: 23209731)
PLoS Pathog. 2018 Jul 17;14(7):e1007166. (PMID: 30016363)
Nucleic Acids Res. 2014 Sep;42(15):10037-49. (PMID: 25106868)
Growth Factors. 2010 Jun;28(3):178-90. (PMID: 20166898)
Curr Opin Virol. 2014 Dec;9:74-83. (PMID: 25462437)
Oncogene. 2004 Nov 11;23(53):8673-80. (PMID: 15467755)
Virology. 1996 Sep 1;223(1):79-88. (PMID: 8806542)
J Inflamm (Lond). 2015 Jul 16;12:42. (PMID: 26180518)
PLoS Pathog. 2015 Mar 27;11(3):e1004750. (PMID: 25816318)
Blood. 2000 Mar 15;95(6):1891-9. (PMID: 10706852)
Nat Immunol. 2017 Jun;18(6):683-693. (PMID: 28394372)
Virology. 1996 Mar 1;217(1):220-9. (PMID: 8599206)
PLoS One. 2012;7(6):e39159. (PMID: 22720057)
Nucleic Acids Res. 2020 Jul 27;48(13):7371-7384. (PMID: 32556261)
J Virol. 1998 Dec;72(12):9729-37. (PMID: 9811707)
فهرسة مساهمة: Keywords: ZFP36L1; antiviral mechanism; flavivirus; zinc-finger RNA-binding protein
المشرفين على المادة: 0 (3' Untranslated Regions)
0 (Butyrate Response Factor 1)
0 (Microtubule-Associated Proteins)
0 (RNA, Viral)
0 (RNA-Binding Proteins)
0 (ZFP36L1 protein, human)
EC 3.1.- (Exoribonucleases)
EC 3.1.- (Exosome Multienzyme Ribonuclease Complex)
EC 3.1.13.1 (XRN1 protein, human)
تواريخ الأحداث: Date Created: 20211013 Date Completed: 20220221 Latest Revision: 20220716
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8754223
DOI: 10.1128/JVI.01665-21
PMID: 34643435
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5514
DOI:10.1128/JVI.01665-21