دورية أكاديمية
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Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga.

التفاصيل البيبلوغرافية
العنوان: Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga.
المؤلفون: Chimetto Tonon LA; Instituto de Química de São Carlos, Universidade de São Paulo, CP 780, CEP 13560-970, São Carlos, SP, Brazil; Instituto de Biologia, SAGE-COPPE, Universidade Federal do Rio de Janeiro (UFRJ), RJ, Brazil. Electronic address: luciane.chimetto@gmail.com., Rua C; Instituto de Química de São Carlos, Universidade de São Paulo, CP 780, CEP 13560-970, São Carlos, SP, Brazil; Instituto de Biologia, SAGE-COPPE, Universidade Federal do Rio de Janeiro (UFRJ), RJ, Brazil., Crnkovic CM; Instituto de Química de São Carlos, Universidade de São Paulo, CP 780, CEP 13560-970, São Carlos, SP, Brazil; Departamento de Tecnologia Bioquímico-Farmacêutica (FBT), Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brazil., Bernardi DI; Instituto de Química de São Carlos, Universidade de São Paulo, CP 780, CEP 13560-970, São Carlos, SP, Brazil., Pires Junior OR; Laboratório de Toxinologia, Instituto de Biologia, Universidade de Brasília, Brasília, DF, Brazil., Haddad CFB; Departamento de Biodiversidade e Centro de Aquicultura, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil., Pedrosa CSG; Instituto D'Or de Pesquisa e Ensino (IDOR), RJ, Brazil., Souza LRQ; Instituto D'Or de Pesquisa e Ensino (IDOR), RJ, Brazil., Rehen SK; Instituto D'Or de Pesquisa e Ensino (IDOR), RJ, Brazil; Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), RJ, Brazil., de Azevedo GPR; Instituto de Biologia, SAGE-COPPE, Universidade Federal do Rio de Janeiro (UFRJ), RJ, Brazil., Thompson CC; Instituto de Biologia, SAGE-COPPE, Universidade Federal do Rio de Janeiro (UFRJ), RJ, Brazil., Thompson FL; Instituto de Biologia, SAGE-COPPE, Universidade Federal do Rio de Janeiro (UFRJ), RJ, Brazil. Electronic address: fabianothompson1@gmail.com., Berlinck RGS; Instituto de Química de São Carlos, Universidade de São Paulo, CP 780, CEP 13560-970, São Carlos, SP, Brazil. Electronic address: rgsberlinck@iqsc.usp.br.
المصدر: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2021 Nov; Vol. 203, pp. 139-146. Date of Electronic Publication: 2021 Oct 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3150 (Electronic) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford ; New York : Pergamon Press, c1962-
مواضيع طبية MeSH: Eugenia* , Microbiota*, Animals ; Anura ; Bacteria ; Tetrodotoxin/toxicity
مستخلص: The genus Brachycephalus includes small species of aposematic anurans known as microendemic, occurring in the mountains of the Atlantic Forest. Brachycephalus ephippium, B. nodoterga and B. pernix have been reported to contain the neurotoxin tetrodotoxin in skin and viscera. The biological conservation of several Brachycephalus species is currently threatened by climate change, deforestation, and the pandemic caused by the fungus Batrachochytrium dendrobatidis (Bd). Despite the well-known importance of amphibians' associated bacteria in the defensive role against pathogens, there is still a poor understanding of amphibian microbiome composition. The present study investigated the composition of B. pitanga microbial community and the presence of TTX in the host and in cultures of bacterial isolates, using a combination of metagenomics, bacterial culture isolation, mass spectrometry and metabolomic analyses. Results of culture-dependent and -independent analyses characterized the microbial communities associated with the skin and viscera of B. pitanga. Mass spectrometry analysis indicated the presence of TTX in host tissues, while bacterial production of TTX was not observed under the experimental conditions used in this investigation. This is the first report confirming the occurrence of TTX in B. pitanga.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Brachycephalidae; Metabolomics; Metagenomics; Microbiota; Toxin
المشرفين على المادة: 4368-28-9 (Tetrodotoxin)
تواريخ الأحداث: Date Created: 20211015 Date Completed: 20211109 Latest Revision: 20211109
رمز التحديث: 20240628
DOI: 10.1016/j.toxicon.2021.10.002
PMID: 34653444
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3150
DOI:10.1016/j.toxicon.2021.10.002