دورية أكاديمية
Editing of DNA methylation using CRISPR/Cas9 and a ssDNA template in human cells.
| العنوان: | Editing of DNA methylation using CRISPR/Cas9 and a ssDNA template in human cells. |
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| المؤلفون: | Katayama S; IMRA Japan Co., Ltd., Sapporo, 004-0015, Japan; AISIN Co., Ltd., 2-1 Asahi-cho, Kariya, 448-8650, Japan. Electronic address: shota.katayama@imra-japan.com., Andou M; IMRA Japan Co., Ltd., Sapporo, 004-0015, Japan. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Dec 03; Vol. 581, pp. 20-24. Date of Electronic Publication: 2021 Oct 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | DNA Methylation* , Epigenesis, Genetic*, DNA, Single-Stranded/*genetics , Gene Editing/*methods , Neoplasm Proteins/*genetics , Sp3 Transcription Factor/*genetics, Base Sequence ; CRISPR-Associated Protein 9/genetics ; CRISPR-Associated Protein 9/metabolism ; CRISPR-Cas Systems ; Clone Cells ; Cytosine/metabolism ; DNA, Single-Stranded/metabolism ; Gene Knock-In Techniques ; Genome ; HEK293 Cells ; Humans ; Neoplasm Proteins/metabolism ; Plasmids/chemistry ; Plasmids/metabolism ; Promoter Regions, Genetic ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Sp3 Transcription Factor/metabolism ; Transcription, Genetic |
| مستخلص: | Programmable DNA methylation is required for understanding of transcriptional regulation and elucidating gene functions. We previously reported that MMEJ-based promoter replacement enabled targeted DNA methylation in human cells. ssDNA-mediated knock-in has recently been reported to completely reduce random integrations. We speculated that by changing MMEJ-to ssDNA-based knock-in, targeted DNA methylation may be achieved through a hemimethylation-symmetric methylation pathway. We herein successfully developed a new system that enables the replacement of an unmethylated promoter with a methylated ssDNA promoter through ssDNA-based knock-in. A DNA methylation ratio of approximately 100% was achieved at the cancer-associated gene SP3 in HEK293 cells. The present results provide a promising framework for artificial epigenetic modifications. Competing Interests: Declaration of competing interest The authors declare no conflicts of interest. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: DNA methylation; Manipulation; Transcription |
| المشرفين على المادة: | 0 (DNA, Single-Stranded) 0 (Neoplasm Proteins) 0 (RNA, Guide, CRISPR-Cas Systems) 0 (SP3 protein, human) 148710-94-5 (Sp3 Transcription Factor) 8J337D1HZY (Cytosine) EC 3.1.- (CRISPR-Associated Protein 9) |
| تواريخ الأحداث: | Date Created: 20211015 Date Completed: 20220103 Latest Revision: 20240104 |
| رمز التحديث: | 20240104 |
| DOI: | 10.1016/j.bbrc.2021.10.018 |
| PMID: | 34653674 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2021.10.018 |