دورية أكاديمية
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Carbon starvation-induced synthesis of GDH2 and PEPCK is essential for the survival of Pichia pastoris.

التفاصيل البيبلوغرافية
العنوان: Carbon starvation-induced synthesis of GDH2 and PEPCK is essential for the survival of Pichia pastoris.
المؤلفون: Dey T; Department of Biochemistry, Indian Institute of Science, Bangalore, 560012, India., Rangarajan PN; Department of Biochemistry, Indian Institute of Science, Bangalore, 560012, India. Electronic address: pnr@iisc.ac.in.
المصدر: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Dec 03; Vol. 581, pp. 25-30. Date of Electronic Publication: 2021 Oct 10.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
أسماء مطبوعة: Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Carbon/*deficiency , Fungal Proteins/*genetics , Glutamate Dehydrogenase (NADP+)/*genetics , Phosphoenolpyruvate Carboxykinase (ATP)/*genetics , RNA, Messenger/*genetics , Saccharomycetales/*drug effects, Amino Acids/metabolism ; Autophagy/genetics ; Autophagy-Related Proteins ; Carbon/pharmacology ; Fungal Proteins/agonists ; Fungal Proteins/biosynthesis ; Gene Expression Regulation, Fungal ; Glutamate Dehydrogenase (NADP+)/biosynthesis ; Metabolism/genetics ; Microbial Viability ; Phosphoenolpyruvate Carboxykinase (ATP)/biosynthesis ; Protein Biosynthesis ; RNA, Messenger/agonists ; RNA, Messenger/biosynthesis ; Saccharomycetales/enzymology ; Saccharomycetales/genetics ; Saccharomycetales/growth & development
مستخلص: The industrial yeast Pichia pastoris can utilize amino acids as the sole source of carbon. It possesses a post-transcriptional regulatory circuit that governs the synthesis of cytosolic glutamate dehydrogenase 2 (GDH2) and phosphoenolpyruvate carboxykinase (PEPCK), key enzymes of amino acid catabolism. Here, we demonstrate that the post-transcriptional regulatory circuit is activated during carbon starvation resulting in the translation of GDH2 and PEPCK mRNAs. GDH2 and PEPCK synthesis is abrogated in Δatg1 indicating a key role for autophagy or an autophagy-related process. Finally, carbon-starved Δgdh2 and Δpepck exhibit poor survival. This study demonstrates a key role for amino acid catabolism during carbon starvation, a phenomenon hitherto unreported in other yeast species.
Competing Interests: Declaration of competing interests The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Pundi Rangarajan reports financial support was provided by Department of Biotechnology (DBT), New Delhi, India. Pundi Rangarajan reports a relationship with Science & Engineering Research Board, New Delhi, India that includes: funding grants. Pundi Rangarajan reports a relationship with DST-FIST that includes: funding grants. Pundi Rangarajan reports a relationship with DBT-IISc partnership that includes: funding grants. None
(Copyright © 2021 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Autophagy; Carbon starvation; Glutamate dehydrogenase 2; Phosphoenolpyruvate carboxykinase; Pichia pastoris
المشرفين على المادة: 0 (Amino Acids)
0 (Autophagy-Related Proteins)
0 (Fungal Proteins)
0 (RNA, Messenger)
7440-44-0 (Carbon)
EC 1.4.1.4 (Glutamate Dehydrogenase (NADP+))
EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP))
SCR Organism: Komagataella pastoris
تواريخ الأحداث: Date Created: 20211015 Date Completed: 20220103 Latest Revision: 20220103
رمز التحديث: 20240628
DOI: 10.1016/j.bbrc.2021.10.015
PMID: 34653675
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2104
DOI:10.1016/j.bbrc.2021.10.015