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Jararhagin-C, a disintegrin-like protein, improves wound healing in mice through stimulation of M2-like macrophage, angiogenesis and collagen deposition.

التفاصيل البيبلوغرافية
العنوان: Jararhagin-C, a disintegrin-like protein, improves wound healing in mice through stimulation of M2-like macrophage, angiogenesis and collagen deposition.
المؤلفون: Ferreira BA; Institute of Biotechnology, Federal University of Uberlândia, UFU, Uberlândia, MG, Brazil; Department of Physiological Sciences, Institute of Biomedical Sciences, Federal University of Uberlândia, UFU, Uberlândia, MG, Brazil., De Moura FBR; Department of Cell Biology, Histology and Embryology, Institute of Biomedical Sciences, Federal University of Uberlândia, UFU, Uberlândia, MG, Brazil., Tomiosso TC; Department of Cell Biology, Histology and Embryology, Institute of Biomedical Sciences, Federal University of Uberlândia, UFU, Uberlândia, MG, Brazil., Corrêa NCR; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil., Goulart LR; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil; Department of Medical Microbiology and Immunology, University of California-Davis, Davis, USA., Barcelos LS; Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Clissa PB; Immunopathology Laboratory, Butantan Institute, Sao Paulo, SP, Brazil., Araújo FA; Department of Physiological Sciences, Institute of Biomedical Sciences, Federal University of Uberlândia, UFU, Uberlândia, MG, Brazil. Electronic address: folaraujo@gmail.com.
المصدر: International immunopharmacology [Int Immunopharmacol] 2021 Dec; Vol. 101 (Pt B), pp. 108224. Date of Electronic Publication: 2021 Oct 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam ; New York : Elsevier Science, c2001-
مواضيع طبية MeSH: Collagen*/metabolism , Crotalid Venoms*/chemistry , Disintegrins*/chemistry , Disintegrins*/pharmacology , Neovascularization, Physiologic*/drug effects , Wound Healing*/drug effects, Animals ; Humans ; Mice ; Cell Survival/drug effects ; Cytokines/genetics ; Cytokines/metabolism ; Gene Expression Regulation/drug effects ; Human Umbilical Vein Endothelial Cells ; Leukocytes/drug effects ; Leukocytes/physiology ; Macrophages ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Bothrops jararaca Venom
مستخلص: Jararhagin-C (Jar-C) is a disintegrin-like protein, isolated from the venom of B. jararaca, with affinity for α 2 β 1 integrin and the ability to incite processes such as angiogenesis and collagen deposition in vivo. Thus, we raised the hypothesis that this protein could be used as a therapeutic strategy for stimulating the healing of excisional wounds in mice. Four wounds were made on the back of Swiss mice, treated with daily intradermal injections of PBS (control group) or Jar-C (200 ng). Ten animals from each experimental group were euthanized and the tissue from the wounds and skin around them were collected for further biochemical, histological and molecular analysis. Wounds treated with Jar-C showed a faster closure rate, accompanied by a reduction in neutrophil infiltrate (MPO), pro-inflammatory cytokine levels (TNF, CXCL1 and CCL2) and an accumulation of macrophages in the analyzed tissues. It was also observed a greater expression of genes associated with the phenotype of alternatively activated macrophages (M2). Concomitantly, the administration of Jar-C holds an angiogenic potential, increasing the density of blood vessels and the synthesis of pro-angiogenic cytokines (VEGF and FGF). We also observed an increase in collagen deposition, accompanied by higher levels of the pro-fibrogenic cytokine TGF-β1. Our data suggests Jar-C stimulates wound healing through stimulation of M2-like macrophage, angiogenesis and collagen deposition. Jar-C may be explored as a therapeutic strategy for wound healing, including the treatment of chronic wounds, where processes such as inflammation, angiogenesis and the deposition / remodeling of the matrix constituents are unregulated.
(Copyright © 2021. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: Cytokines; Disintegrin; Fibrogenesis; Macrophage polarization; Pro-angiogenic
المشرفين على المادة: 9007-34-5 (Collagen)
0 (Crotalid Venoms)
0 (Cytokines)
0 (Disintegrins)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20211016 Date Completed: 20220322 Latest Revision: 20240229
رمز التحديث: 20240229
DOI: 10.1016/j.intimp.2021.108224
PMID: 34655846
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-1705
DOI:10.1016/j.intimp.2021.108224