دورية أكاديمية
DNA Origami Voltage Sensors for Transmembrane Potentials with Single-Molecule Sensitivity.
| العنوان: | DNA Origami Voltage Sensors for Transmembrane Potentials with Single-Molecule Sensitivity. |
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| المؤلفون: | Ochmann SE; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, 81377 München, Germany., Joshi H; Department of Physics and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana, Illinois 61820, United States., Büber E; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, 81377 München, Germany., Franquelim HG; Max Planck Institute of Biochemistry, 82152 Planegg, Germany., Stegemann P; Center of Medical Biotechnology (ZMB) and Center for Nano Integration Duisburg-Essen (CENIDE), University of Duisburg-Essen, 45117 Essen, Germany., Saccà B; Center of Medical Biotechnology (ZMB) and Center for Nano Integration Duisburg-Essen (CENIDE), University of Duisburg-Essen, 45117 Essen, Germany., Keyser UF; Cavendish Laboratory, Department of Physics, University of Cambridge, JJ Thomson Avenue, Cambridge, CB3 0HE, United Kingdom., Aksimentiev A; Department of Physics and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana, Illinois 61820, United States., Tinnefeld P; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, 81377 München, Germany. |
| المصدر: | Nano letters [Nano Lett] 2021 Oct 27; Vol. 21 (20), pp. 8634-8641. Date of Electronic Publication: 2021 Oct 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101088070 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-6992 (Electronic) Linking ISSN: 15306984 NLM ISO Abbreviation: Nano Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Chemical Society, c2001- |
| مواضيع طبية MeSH: | Fluorescence Resonance Energy Transfer* , Nanotechnology*, DNA/genetics ; Fluorescent Dyes ; Membrane Potentials ; Neurons |
| مستخلص: | Signal transmission in neurons goes along with changes in the transmembrane potential. To report them, different approaches, including optical voltage-sensing dyes and genetically encoded voltage indicators, have evolved. Here, we present a DNA nanotechnology-based system and demonstrated its functionality on liposomes. Using DNA origami, we incorporated and optimized different properties such as membrane targeting and voltage sensing modularly. As a sensing unit, we used a hydrophobic red dye anchored to the membrane and an anionic green dye at the DNA to connect the nanostructure and the membrane dye anchor. Voltage-induced displacement of the anionic donor unit was read out by fluorescence resonance energy transfer (FRET) changes of single sensors attached to liposomes. A FRET change of ∼5% for ΔΨ = 100 mV was observed. The working mechanism of the sensor was rationalized by molecular dynamics simulations. Our approach holds potential for an application as nongenetically encoded membrane sensors. |
| فهرسة مساهمة: | Keywords: DNA origami; molecular dynamic simulations; single-molecule FRET; transmembrane potential; voltage imaging; voltage sensor |
| المشرفين على المادة: | 0 (Fluorescent Dyes) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20211018 Date Completed: 20211110 Latest Revision: 20211110 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/acs.nanolett.1c02584 |
| PMID: | 34662130 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1530-6992 |
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| DOI: | 10.1021/acs.nanolett.1c02584 |