دورية أكاديمية
Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders.
العنوان: | Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders. |
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المؤلفون: | Alves JRS; Molecular Biology and Malaria Immunology, René Rachou Institute, Oswaldo Cruz Foundation (FIOCRUZ), Belo Horizonte, Brazil., de Araújo FF; Integrated Research Group in Biomarkers, René Rachou Institute, Oswaldo Cruz Foundation (FIOCRUZ), Belo Horizonte, Brazil., Pires CV; Center for Global Health and Infectious Diseases Research, Department of Global Health, College of Public Health, University of South Florida, Tampa, FL, United States., Teixeira-Carvalho A; Integrated Research Group in Biomarkers, René Rachou Institute, Oswaldo Cruz Foundation (FIOCRUZ), Belo Horizonte, Brazil., Lima BAS; Molecular Biology and Malaria Immunology, René Rachou Institute, Oswaldo Cruz Foundation (FIOCRUZ), Belo Horizonte, Brazil., Torres LM; Molecular Biology and Malaria Immunology, René Rachou Institute, Oswaldo Cruz Foundation (FIOCRUZ), Belo Horizonte, Brazil., Ntumngia FB; Center for Global Health and Infectious Diseases Research, Department of Global Health, College of Public Health, University of South Florida, Tampa, FL, United States., Adams JH; Center for Global Health and Infectious Diseases Research, Department of Global Health, College of Public Health, University of South Florida, Tampa, FL, United States., Kano FS; Molecular Biology and Malaria Immunology, René Rachou Institute, Oswaldo Cruz Foundation (FIOCRUZ), Belo Horizonte, Brazil., Carvalho LH; Molecular Biology and Malaria Immunology, René Rachou Institute, Oswaldo Cruz Foundation (FIOCRUZ), Belo Horizonte, Brazil. |
المصدر: | Frontiers in immunology [Front Immunol] 2021 Oct 05; Vol. 12, pp. 704653. Date of Electronic Publication: 2021 Oct 05 (Print Publication: 2021). |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
مواضيع طبية MeSH: | Antibodies, Protozoan*/immunology , Flow Cytometry*, Antibodies, Neutralizing/*immunology , Antigens, Protozoan/*immunology , Malaria, Vivax/*immunology , Plasmodium vivax/*immunology , Protozoan Proteins/*immunology , Receptors, Cell Surface/*immunology, Humans ; Malaria, Vivax/diagnosis |
مستخلص: | Malaria remains a major public health problem worldwide, and Plasmodium vivax is the most widely distributed malaria parasite. Naturally acquired binding inhibitory antibodies (BIAbs) to region II of the Duffy binding protein (DBPII), a P. vivax ligand that is critical for reticulocyte invasion, are associated with a reduced risk of clinical malaria. Owing to methodological issues in evaluating antibodies that inhibit the DBPII-DARC interaction, a limited number of studies have investigated DBPII BIAbs in P. vivax -exposed populations. Based on the assumption that individuals with a consistent BIAb response are characterized by strain-transcending immune responses, we hypothesized that detecting broadly reactive DBPII antibodies would indicate the presence of BIAb response. By taking advantage of an engineered DBPII immunogen targeting conserved DBPII neutralizing epitopes (DEKnull-2), we standardized a multiplex flow cytometry-based serological assay to detect broadly neutralizing IgG antibodies. For this study, a standard in vitro cytoadherence assay with COS-7 cells expressing DBPII was used to test for DBPII BIAb response in long-term P. vivax- exposed Amazonian individuals. Taken together, the results demonstrate that this DBPII-based multiplex assay facilitates identifying DBPII BIAb carriers. Of relevance, the ability of the multiplex assay to identify BIAb responders was highly accurate when the positivity for all antigens was considered. In conclusion, the standardized DBPII-based flow cytometric assay confirmed that DBPII-BIAb activity was associated with the breadth rather than the magnitude of anti-DBPII antibodies. Altogether, our results suggest that multiplex detection of broadly DBPII-reactive antibodies facilitates preliminary screening of BIAb responders. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Alves, de Araújo, Pires, Teixeira-Carvalho, Lima, Torres, Ntumngia, Adams, Kano and Carvalho.) |
References: | J Exp Med. 1994 Aug 1;180(2):497-506. (PMID: 8046329) mBio. 2016 Aug 23;7(4):. (PMID: 27555313) Expert Rev Vaccines. 2021 Feb;20(2):97-112. (PMID: 33481638) PLoS Negl Trop Dis. 2015 Jan 08;9(1):e3413. (PMID: 25569135) Mol Biochem Parasitol. 1991 Jan;44(1):125-32. (PMID: 1849231) PLoS Negl Trop Dis. 2016 Dec 13;10(12):e0005177. (PMID: 27959918) Parasitol Int. 2014 Dec;63(6):858-64. (PMID: 25108177) Front Immunol. 2015 Jul 08;6:348. (PMID: 26217340) Proc Natl Acad Sci U S A. 2008 Jun 17;105(24):8363-8. (PMID: 18523022) Clin Vaccine Immunol. 2012 Jan;19(1):30-6. (PMID: 22116684) Trop Med Int Health. 2012 Aug;17(8):989-1000. (PMID: 22643072) Clin Vaccine Immunol. 2006 Dec;13(12):1307-13. (PMID: 17035513) PLoS One. 2014 Apr 07;9(4):e93782. (PMID: 24710306) Clin Exp Immunol. 2009 Jun;156(3):502-10. (PMID: 19438604) Lancet. 2019 Jul 27;394(10195):332-343. (PMID: 31229233) Sci Rep. 2017 Oct 23;7(1):13779. (PMID: 29062081) Nat Microbiol. 2019 Sep;4(9):1497-1507. (PMID: 31133755) PLoS Negl Trop Dis. 2019 Feb 15;13(2):e0006987. (PMID: 30768655) Clin Diagn Lab Immunol. 2004 Mar;11(2):272-9. (PMID: 15013975) Hum Vaccin. 2010 Jan;6(1):124-32. (PMID: 20009526) Malar J. 2010 Nov 22;9:334. (PMID: 21092207) PLoS Negl Trop Dis. 2015 Mar 20;9(3):e0003644. (PMID: 25793371) Infect Immun. 2009 Sep;77(9):4009-17. (PMID: 19564376) PLoS One. 2012;7(4):e35769. (PMID: 22558221) Malar J. 2018 Nov 9;17(1):417. (PMID: 30413163) Public Health Rep. 2009 May-Jun;124(3):471-4. (PMID: 19445426) PLoS One. 2011;6(8):e22944. (PMID: 21829672) J Immunoassay Immunochem. 2012;33(4):403-13. (PMID: 22963489) Int J Parasitol. 2012 Nov;42(12):1055-62. (PMID: 23068913) PLoS One. 2020 May 7;15(5):e0232786. (PMID: 32379804) Curr Opin Hematol. 2015 May;22(3):220-6. (PMID: 25767956) Science. 2018 Jan 5;359(6371):48-55. (PMID: 29302006) J Infect Dis. 2016 Nov 15;214(10):1539-1546. (PMID: 27578850) Ann Trop Med Parasitol. 1995 Apr;89(2):113-20. (PMID: 7605120) Infect Immun. 1994 Dec;62(12):5581-6. (PMID: 7960140) PLoS One. 2018 Nov 12;13(11):e0207244. (PMID: 30419071) Proc Natl Acad Sci U S A. 2016 May 31;113(22):6277-82. (PMID: 27194724) Am J Trop Med Hyg. 2005 Jun;72(6):675-81. (PMID: 15964949) Am J Clin Pathol. 2002 Apr;117(4):589-96. (PMID: 11939734) PLoS Negl Trop Dis. 2017 Sep 11;11(9):e0005888. (PMID: 28892517) Infect Immun. 2012 Mar;80(3):1203-8. (PMID: 22215740) J Parasitol. 2002 Jun;88(3):521-35. (PMID: 12099421) PLoS Pathog. 2013;9(6):e1003420. (PMID: 23853575) Infect Immun. 2003 May;71(5):2508-15. (PMID: 12704122) Am J Trop Med Hyg. 2020 Nov;103(5):1871-1882. (PMID: 32959760) Lancet Infect Dis. 2009 Sep;9(9):555-66. (PMID: 19695492) J Exp Med. 1996 Oct 1;184(4):1531-6. (PMID: 8879225) Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7085-9. (PMID: 1496004) BMJ Glob Health. 2017 Aug 19;2(3):e000415. (PMID: 29082022) Cell Microbiol. 2020 Jan;22(1):e13110. (PMID: 31469946) Malar J. 2010 Sep 07;9:254. (PMID: 20822506) Infect Immun. 2000 Jun;68(6):3164-71. (PMID: 10816459) N Biotechnol. 2015 Sep 25;32(5):521-32. (PMID: 25917978) Trends Parasitol. 2018 Feb;34(2):114-126. (PMID: 29153587) Nat Microbiol. 2021 Aug;6(8):991-999. (PMID: 34294905) Scand J Immunol. 2008 Mar;67(3):270-8. (PMID: 18226014) |
معلومات مُعتمدة: | R01 AI064478 United States AI NIAID NIH HHS |
فهرسة مساهمة: | Keywords: Duffy binding protein; Plasmodium vivax; inhibitory antibodies; malaria; multiplex assay |
المشرفين على المادة: | 0 (Antibodies, Neutralizing) 0 (Antibodies, Protozoan) 0 (Antigens, Protozoan) 0 (Duffy antigen binding protein, Plasmodium) 0 (Protozoan Proteins) 0 (Receptors, Cell Surface) |
تواريخ الأحداث: | Date Created: 20211022 Date Completed: 20211221 Latest Revision: 20231107 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8523986 |
DOI: | 10.3389/fimmu.2021.704653 |
PMID: | 34675915 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1664-3224 |
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DOI: | 10.3389/fimmu.2021.704653 |