دورية أكاديمية
أعرض في EDS

Adaptation and genomic erosion in fragmented Pseudomonas aeruginosa populations in the sinuses of people with cystic fibrosis.

التفاصيل البيبلوغرافية
العنوان: Adaptation and genomic erosion in fragmented Pseudomonas aeruginosa populations in the sinuses of people with cystic fibrosis.
المؤلفون: Armbruster CR; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Marshall CW; Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA., Garber AI; Biodesign Center for Mechanisms of Evolution and School of Life Sciences, Arizona State University, Tempe, AZ 85281, USA., Melvin JA; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Zemke AC; Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Moore J; Department of Otolaryngology, University of Pittsburgh Medical Center, Pittsburgh, PA 15219, USA., Zamora PF; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Li K; Center for Medicine and the Microbiome, University of Pittsburgh and University of Pittsburgh Medical Center, Pittsburgh, PA 15219, USA., Fritz IL; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Manko CD; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Weaver ML; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Gaston JR; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Morris A; Center for Medicine and the Microbiome, University of Pittsburgh and University of Pittsburgh Medical Center, Pittsburgh, PA 15219, USA., Methé B; Center for Medicine and the Microbiome, University of Pittsburgh and University of Pittsburgh Medical Center, Pittsburgh, PA 15219, USA., DePas WH; Department of Pediatrics, Children's Hospital of Pittsburgh and University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Lee SE; Department of Otolaryngology, University of Pittsburgh Medical Center, Pittsburgh, PA 15219, USA. Electronic address: slee192@bwh.harvard.edu., Cooper VS; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA; Center for Medicine and the Microbiome, University of Pittsburgh and University of Pittsburgh Medical Center, Pittsburgh, PA 15219, USA; Pittsburgh Center for Evolutionary Biology & Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA. Electronic address: vaughn.cooper@pitt.edu., Bomberger JM; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA. Electronic address: jbomb@pitt.edu.
المصدر: Cell reports [Cell Rep] 2021 Oct 19; Vol. 37 (3), pp. 109829.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, c 2012-
مواضيع طبية MeSH: Evolution, Molecular* , Genome, Bacterial* , Mutation*, Cystic Fibrosis/*microbiology , Paranasal Sinuses/*microbiology , Pseudomonas Infections/*microbiology , Pseudomonas aeruginosa/*genetics, Adult ; Cell Line ; Cystic Fibrosis/diagnosis ; Female ; Genetic Drift ; Genotype ; Humans ; Longitudinal Studies ; Male ; Phenotype ; Phylogeny ; Prospective Studies ; Pseudomonas Infections/diagnosis ; Pseudomonas aeruginosa/growth & development
مستخلص: Pseudomonas aeruginosa notoriously adapts to the airways of people with cystic fibrosis (CF), yet how infection-site biogeography and associated evolutionary processes vary as lifelong infections progress remains unclear. Here we test the hypothesis that early adaptations promoting aggregation influence evolutionary-genetic trajectories by examining longitudinal P. aeruginosa from the sinuses of six adults with CF. Highly host-adapted lineages harbored mutator genotypes displaying signatures of early genome degradation associated with recent host restriction. Using an advanced imaging technique (MiPACT-HCR [microbial identification after passive clarity technique]), we find population structure tracks with genome degradation, with the most host-adapted, genome-degraded P. aeruginosa (the mutators) residing in small, sparse aggregates. We propose that following initial adaptive evolution in larger populations under strong selection for aggregation, P. aeruginosa persists in small, fragmented populations that experience stronger effects of genetic drift. These conditions enrich for mutators and promote degenerative genome evolution. Our findings underscore the importance of infection-site biogeography to pathogen evolution.
Competing Interests: Declaration of interests V.S.C. is a founder of Microbial Genome Sequencing Center, LLC (MiGS) and is a scientific advisor for MiGS.
(Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: R61 HL137077 United States HL NHLBI NIH HHS; U01 AI124302 United States AI NIAID NIH HHS; R01 HL123771 United States HL NHLBI NIH HHS; P30 DK072506 United States DK NIDDK NIH HHS; R01 HL142587 United States HL NHLBI NIH HHS; R21 HL143091 United States HL NHLBI NIH HHS; L40 HL104736 United States HL NHLBI NIH HHS; T32 HL129949 United States HL NHLBI NIH HHS; R33 HL137077 United States HL NHLBI NIH HHS; T32 AI049820 United States AI NIAID NIH HHS; R01 HL138630 United States HL NHLBI NIH HHS; T32 AI060525 United States AI NIAID NIH HHS; UL1 TR000005 United States TR NCATS NIH HHS; T35 DK065521 United States DK NIDDK NIH HHS; R00 HL098342 United States HL NHLBI NIH HHS; K23 HL131930 United States HL NHLBI NIH HHS
فهرسة مساهمة: Keywords: Pseudomonas aeruginosa; biofilm; chronic rhinosinusitis; cystic fibrosis; genome degradation; host restriction; hybrid assembly; pathoadaptation; pseudogene; sinus
تواريخ الأحداث: Date Created: 20211023 Date Completed: 20220215 Latest Revision: 20240404
رمز التحديث: 20240404
مُعرف محوري في PubMed: PMC8667756
DOI: 10.1016/j.celrep.2021.109829
PMID: 34686349
قاعدة البيانات: MEDLINE
الوصف
تدمد:2211-1247
DOI:10.1016/j.celrep.2021.109829