دورية أكاديمية
Functional single-cell genomics of human cytomegalovirus infection.
| العنوان: | Functional single-cell genomics of human cytomegalovirus infection. |
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| المؤلفون: | Hein MY; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA. marco.hein@czbiohub.org.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA. marco.hein@czbiohub.org.; Chan Zuckerberg Biohub, San Francisco, CA, USA. marco.hein@czbiohub.org., Weissman JS; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA. weissman@wi.mit.edu.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA. weissman@wi.mit.edu.; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, USA. weissman@wi.mit.edu. |
| المصدر: | Nature biotechnology [Nat Biotechnol] 2022 Mar; Vol. 40 (3), pp. 391-401. Date of Electronic Publication: 2021 Oct 25. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature America Publishing Country of Publication: United States NLM ID: 9604648 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1546-1696 (Electronic) Linking ISSN: 10870156 NLM ISO Abbreviation: Nat Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Nature America Publishing Original Publication: New York, NY : Nature Pub. Co., [1996- |
| مواضيع طبية MeSH: | Cytomegalovirus Infections*/genetics , Genomics*, Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; Fibroblasts ; Host-Pathogen Interactions/genetics ; Humans |
| مستخلص: | Understanding how viral and host factors interact and how perturbations impact infection is the basis for designing antiviral interventions. Here we define the functional contribution of each viral and host factor involved in human cytomegalovirus infection in primary human fibroblasts through pooled CRISPR interference and nuclease screening. To determine how genetic perturbation of critical host and viral factors alters the timing, course and progression of infection, we applied Perturb-seq to record the transcriptomes of tens of thousands of CRISPR-modified single cells and found that, normally, most cells follow a stereotypical transcriptional trajectory. Perturbing critical host factors does not change the stereotypical transcriptional trajectory per se but can stall, delay or accelerate progression along the trajectory, allowing one to pinpoint the stage of infection at which host factors act. Conversely, perturbation of viral factors can create distinct, abortive trajectories. Our results reveal the roles of host and viral factors and provide a roadmap for the dissection of host-pathogen interactions. (© 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.) |
| التعليقات: | Comment in: Nat Microbiol. 2021 Dec;6(12):1477-1478. (PMID: 34697463) |
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| تواريخ الأحداث: | Date Created: 20211026 Date Completed: 20220421 Latest Revision: 20230208 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1038/s41587-021-01059-3 |
| PMID: | 34697476 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1546-1696 |
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| DOI: | 10.1038/s41587-021-01059-3 |