دورية أكاديمية
أعرض في EDS

Loss of Melanopsin (OPN4) Leads to a Faster Cell Cycle Progression and Growth in Murine Melanocytes.

التفاصيل البيبلوغرافية
العنوان: Loss of Melanopsin (OPN4) Leads to a Faster Cell Cycle Progression and Growth in Murine Melanocytes.
المؤلفون: de Assis LVM; Laboratory of Comparative Physiology of Pigmentation, Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo 05508-090, Brazil., Moraes MN; Laboratory of Comparative Physiology of Pigmentation, Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo 05508-090, Brazil., Mendes D; DNA Repair Lab, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil., Silva MM; DNA Repair Lab, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil., Menck CFM; DNA Repair Lab, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil., Castrucci AML; Laboratory of Comparative Physiology of Pigmentation, Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo 05508-090, Brazil.; Department of Biology, University of Virginia, Charlottesville, VA 22904, USA.
المصدر: Current issues in molecular biology [Curr Issues Mol Biol] 2021 Oct 04; Vol. 43 (3), pp. 1436-1450. Date of Electronic Publication: 2021 Oct 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100931761 Publication Model: Electronic Cited Medium: Internet ISSN: 1467-3045 (Electronic) Linking ISSN: 14673037 NLM ISO Abbreviation: Curr Issues Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2021- : Basel, Switzerland : MDPI
Original Publication: Wymondham, Norfolk, UK : Caister Academic Press,
مواضيع طبية MeSH: Cell Cycle/*genetics , Melanocytes/*metabolism , Rod Opsins/*deficiency, Animals ; Biomarkers ; CLOCK Proteins/genetics ; Cell Cycle/drug effects ; Cell Cycle Proteins/genetics ; Cell Proliferation ; Cells, Cultured ; Flow Cytometry ; Gene Expression Regulation/drug effects ; Gene Knockout Techniques ; Melanocytes/drug effects ; Mice ; Skin/cytology ; Skin/metabolism
مستخلص: Skin melanocytes harbor a complex photosensitive system comprised of opsins, which were shown, in recent years, to display light- and thermo-independent functions. Based on this premise, we investigated whether melanopsin, OPN4, displays such a role in normal melanocytes. In this study, we found that murine Opn4 KO melanocytes displayed a faster proliferation rate compared to Opn4 WT melanocytes. Cell cycle population analysis demonstrated that OPN4 KO melanocytes exhibited a faster cell cycle progression with reduced G 0 -G 1 , and highly increased S and slightly increased G 2 /M cell populations compared to the Opn4 WT counterparts. Expression of specific cell cycle-related genes in Opn4 KO melanocytes exhibited alterations that corroborate a faster cell cycle progression. We also found significant modification in gene and protein expression levels of important regulators of melanocyte physiology. PER1 protein level was higher while BMAL1 and REV-ERBα decreased in Opn4 KO melanocytes compared to Opn4 WT cells. Interestingly, the gene expression of microphthalmia-associated transcription factor (MITF) was upregulated in Opn4 KO melanocytes, which is in line with a higher proliferative capability. Taken altogether, we demonstrated that OPN4 regulates cell proliferation, cell cycle, and affects the expression of several important factors of the melanocyte physiology; thus, arguing for a putative tumor suppression role in melanocytes.
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معلومات مُعتمدة: 2017/24615-5 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2018/14728-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2019/19435-3 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2017/26651-9 Fundação de Amparo à Pesquisa do Estado de São Paulo
فهرسة مساهمة: Keywords: melanocytes; melanopsin; molecular clock; opsins; proliferation and cell cycle; skin biology
المشرفين على المادة: 0 (Biomarkers)
0 (Cell Cycle Proteins)
0 (Rod Opsins)
0 (melanopsin)
EC 2.3.1.48 (CLOCK Proteins)
EC 2.3.1.48 (Clock protein, mouse)
تواريخ الأحداث: Date Created: 20211026 Date Completed: 20220215 Latest Revision: 20220716
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8929055
DOI: 10.3390/cimb43030101
PMID: 34698095
قاعدة البيانات: MEDLINE
الوصف
تدمد:1467-3045
DOI:10.3390/cimb43030101