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Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?

التفاصيل البيبلوغرافية
العنوان: Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?
المؤلفون: Madeira FF; Laboratório de Biologia Celular, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Instituto de Biociências, Letras e Ciências Exatas, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, 15054-000, Brasil., Delgado LMG; Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Instituto de Biociências Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu, SP, 18618-689, Brasil., Bittinelli IF; Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Instituto de Biociências Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu, SP, 18618-689, Brasil., de Oliveira J; Laboratório de Parasitologia, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Faculdade de Ciências Farmacêuticas, Rodovia Araraquara-Jaú km 1, Araraquara, SP, 14801-902, Brasil.; Laboratório de Entomologia em Saúde Pública, Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, Av. Dr. Arnaldo 715, São Paulo, SP, Brasil., Ravazi A; Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Instituto de Biociências Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu, SP, 18618-689, Brasil., Dos Reis YV; Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Instituto de Biociências Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu, SP, 18618-689, Brasil., de Oliveira ABB; Laboratório de Biologia Celular, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Instituto de Biociências, Letras e Ciências Exatas, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, 15054-000, Brasil., Cristal DC; Laboratório de Parasitologia, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Faculdade de Ciências Farmacêuticas, Rodovia Araraquara-Jaú km 1, Araraquara, SP, 14801-902, Brasil., Galvão C; Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Pavilhão Rocha Lima, sala 505, Rio de Janeiro, RJ, 21040-360, Brasil. clebergalvao@gmail.com., de Azeredo-Oliveira MTV; Laboratório de Biologia Celular, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Instituto de Biociências, Letras e Ciências Exatas, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, 15054-000, Brasil., da Rosa JA; Laboratório de Parasitologia, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Faculdade de Ciências Farmacêuticas, Rodovia Araraquara-Jaú km 1, Araraquara, SP, 14801-902, Brasil., Alevi KCC; Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Instituto de Biociências Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu, SP, 18618-689, Brasil.; Laboratório de Parasitologia, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Faculdade de Ciências Farmacêuticas, Rodovia Araraquara-Jaú km 1, Araraquara, SP, 14801-902, Brasil.
المصدر: Parasites & vectors [Parasit Vectors] 2021 Oct 27; Vol. 14 (1), pp. 553. Date of Electronic Publication: 2021 Oct 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Chromosomes* , Polymorphism, Genetic*, Insect Vectors/*genetics , Triatoma/*classification , Triatoma/*genetics, Animals ; Bolivia ; Brazil ; Chagas Disease/transmission ; Cytogenetic Analysis ; Female ; Male ; Phylogeny
مستخلص: Background: Triatoma sordida is one of the main Chagas disease vectors in Brazil. In addition to Brazil, this species has already been reported in Bolivia, Argentina, Paraguay, and Uruguay. It is hypothesized that the insects currently identified as T. sordida are a species subcomplex formed by three cytotypes (T. sordida sensu stricto [s.s.], T. sordida La Paz, and T. sordida Argentina). With the recent description of T. rosai from the Argentinean specimens, it became necessary to assess the taxonomic status of T. sordida from La Paz, Bolivia, since it was suggested that it may represent a new species, which has taxonomic, evolutionary, and epidemiological implications. Based on the above, we carried out molecular and experimental crossover studies to assess the specific status of T. sordida La Paz.
Methods: To evaluate the pre- and postzygotic barriers between T. sordida La Paz and T. sordida s.s., experimental crosses and intercrosses between F1 hybrids and between F2 hybrids were conducted. In addition, cytogenetic analyses of the F1 and F2 hybrids were applied with an emphasis on the degree of pairing between the homeologous chromosomes, and morphological analyses of the male gonads were performed to evaluate the presence of gonadal dysgenesis. Lastly, the genetic distance between T. sordida La Paz and T. sordida s.s. was calculated for the CYTB, ND1, and ITS1 genes.
Results: Regardless of the gene used, T. sordida La Paz showed low genetic distance compared to T. sordida s.s. (below 2%). Experimental crosses resulted in offspring for both directions, demonstrating that there are no prezygotic barriers installed between these allopatric populations. Furthermore, postzygotic barriers were not observed either (since the F1 × F1 and F2 × F2 intercrosses resulted in viable offspring). Morphological and cytogenetic analyses of the male gonads of the F1 and F2 offspring demonstrated that the testes were not atrophied and did not show chromosome pairing errors.
Conclusion: Based on the low genetic distance (which configures intraspecific variation), associated with the absence of prezygotic and postzygotic reproductive barriers, we confirm that T. sordida La Paz represents only a chromosomal polymorphism of T. sordida s.s.
(© 2021. The Author(s).)
References: Zookeys. 2021 Jan 26;1012:71-93. (PMID: 33584109)
Parasit Vectors. 2017 Sep 6;10(1):412. (PMID: 28877741)
Biochem Genet. 2005 Aug;43(7-8):365-73. (PMID: 16187161)
Parasit Vectors. 2014 Mar 31;7:149. (PMID: 24685273)
Infect Genet Evol. 2012 Dec;12(8):1652-3. (PMID: 22760157)
Mem Inst Oswaldo Cruz. 1995 Jul-Aug;90(4):443-8. (PMID: 8551947)
Infect Genet Evol. 2014 Aug;26:123-31. (PMID: 24861813)
Parasit Vectors. 2015 Sep 29;8:495. (PMID: 26419232)
Mol Phylogenet Evol. 2004 Jul;32(1):46-56. (PMID: 15186796)
PLoS One. 2019 Feb 7;14(2):e0211285. (PMID: 30730919)
Mem Inst Oswaldo Cruz. 2001 Oct;96(7):895-9. (PMID: 11685251)
Am J Trop Med Hyg. 2018 Feb;98(2):475-477. (PMID: 29260653)
Infect Genet Evol. 2020 Apr;79:104149. (PMID: 31864008)
Acta Trop. 2013 Oct;128(1):162-7. (PMID: 23850508)
Am J Trop Med Hyg. 2021 Sep 7;:. (PMID: 34491212)
Parasitol Res. 2021 Aug;120(8):2939-2945. (PMID: 34185156)
Mem Inst Oswaldo Cruz. 1999;94 Suppl 1:229-38. (PMID: 10677723)
Mem Inst Oswaldo Cruz. 2015 Apr;110(2):192-200. (PMID: 25946242)
Insects. 2020 Nov 26;11(12):. (PMID: 33255910)
Acta Trop. 2020 Dec;212:105679. (PMID: 32860747)
PLoS Negl Trop Dis. 2012;6(5):e1650. (PMID: 22685616)
Genetica. 2005 Nov;125(2-3):261-70. (PMID: 16247698)
PLoS One. 2014 Feb 03;9(2):e87493. (PMID: 24498330)
فهرسة مساهمة: Keywords: Chagas disease vector; Experimental crosses; Genetic distance; Triatomines
تواريخ الأحداث: Date Created: 20211028 Date Completed: 20220114 Latest Revision: 20220114
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8555079
DOI: 10.1186/s13071-021-04988-9
PMID: 34706767
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-3305
DOI:10.1186/s13071-021-04988-9