دورية أكاديمية
Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis.
| العنوان: | Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis. |
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| المؤلفون: | de Melo EH; Programa de Pós-Graduação em Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, Brazil., Gomes HM; Laboratório de Biologia Molecular Aplicada à Micobactérias, Fundação Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada à Micobactérias, Rio de Janeiro, Brazil., Suffys PN; Laboratório de Biologia Molecular Aplicada à Micobactérias, Fundação Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada à Micobactérias, Rio de Janeiro, Brazil., Lopes MQP; Laboratório de Biologia Molecular Aplicada à Micobactérias, Fundação Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada à Micobactérias, Rio de Janeiro, Brazil., de Figueiredo Teixeira RL; Laboratório de Biologia Molecular Aplicada à Micobactérias, Fundação Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada à Micobactérias, Rio de Janeiro, Brazil., Dos Santos ÍR; Laboratório de Biologia Molecular Aplicada à Micobactérias, Fundação Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada à Micobactérias, Rio de Janeiro, Brazil., Franco MMJ; Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, Brazil., Langoni H; Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, Brazil., Paes AC; Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, Brazil., Afonso JAB; Clínica de Bovinos de Garanhuns, Universidade Federal Rural de Pernambuco, Garanhuns, Brazil., de Mendonça CL; Clínica de Bovinos de Garanhuns, Universidade Federal Rural de Pernambuco, Garanhuns, Brazil. |
| المصدر: | Frontiers in veterinary science [Front Vet Sci] 2021 Oct 11; Vol. 8, pp. 747226. Date of Electronic Publication: 2021 Oct 11 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2014]- |
| مستخلص: | Molecular diagnosis of bovine tuberculosis plays an essential role in the epidemiological knowledge of the disease. Bovine tuberculosis caused by Mycobacterium bovis represents a risk to human health. This study aimed to perform the genotypic characterization of M. bovis isolated from bovines diagnosed as tuberculosis from dairy herds in the state of Pernambuco, Brazil. Granulomas from 30 bovines were sent for microbiological culture, and colonies compatible with Mycobacterium spp. were obtained in at least one culture from 17/30 granulomas. All isolates were confirmed to be M. bovis by spoligotyping and 24 loci MIRU-VNTR typing. While spoligotyping characterized the isolates as SB0121, SB0295, SB0852, SB0120, and an unclassified genotype, 24 loci MIRU-VNTR rendered two clusters of two isolates each and 13 unique profiles. Loci ETR-A showed higher discriminatory power, and loci (ETR-B, ETR-C, MIRU16, MIRU27, and QUB26) showed moderate allelic diversity. This is the first study on the genetic variability of the infectious agent cause of bovine TB in Pernambuco and demonstrates variability of strains in the state. Thus, it corroborates the importance of this microorganism as agent of bovine tuberculosis and its zoonotic potential, this epidemiological tool being a determinant in the rigor of the sanitary practices of disease control in dairy herds. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 de Melo, Gomes, Suffys, Lopes, de Figueiredo Teixeira, dos Santos, Franco, Langoni, Paes, Afonso and de Mendonça.) |
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| فهرسة مساهمة: | Keywords: MIRU-VNTR; Mycobacterium bovis; bovine tuberculosis; dairy cattle; genotyping; pathology; spoligotyping |
| تواريخ الأحداث: | Date Created: 20211028 Latest Revision: 20211030 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8542897 |
| DOI: | 10.3389/fvets.2021.747226 |
| PMID: | 34708105 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2297-1769 |
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| DOI: | 10.3389/fvets.2021.747226 |