دورية أكاديمية
Transcriptional and chromatin-based partitioning mechanisms uncouple protein scaling from cell size.
العنوان: | Transcriptional and chromatin-based partitioning mechanisms uncouple protein scaling from cell size. |
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المؤلفون: | Swaffer MP; Department of Biology, Stanford University, Stanford, CA 94305, USA., Kim J; Department of Biology, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA., Chandler-Brown D; Department of Biology, Stanford University, Stanford, CA 94305, USA., Langhinrichs M; Department of Biology, Stanford University, Stanford, CA 94305, USA., Marinov GK; Department of Genetics, Stanford University, Stanford, CA 94305, USA., Greenleaf WJ; Department of Genetics, Stanford University, Stanford, CA 94305, USA., Kundaje A; Department of Genetics, Stanford University, Stanford, CA 94305, USA., Schmoller KM; Department of Biology, Stanford University, Stanford, CA 94305, USA; Institute of Functional Epigenetics, Helmholtz Zentrum München, 85764 Neuherberg, Germany., Skotheim JM; Department of Biology, Stanford University, Stanford, CA 94305, USA. Electronic address: skotheim@stanford.edu. |
المصدر: | Molecular cell [Mol Cell] 2021 Dec 02; Vol. 81 (23), pp. 4861-4875.e7. Date of Electronic Publication: 2021 Nov 02. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 9802571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4164 (Electronic) Linking ISSN: 10972765 NLM ISO Abbreviation: Mol Cell Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Cambridge Ma : Cell Press Original Publication: Cambridge, Mass. : Cell Press, c1997- |
مواضيع طبية MeSH: | Gene Expression Regulation, Fungal* , Transcription, Genetic*, Chromatin/*chemistry , Saccharomyces cerevisiae/*metabolism , Schizosaccharomyces/*metabolism, Cell Cycle ; Chromatin/metabolism ; Computational Biology ; Histones/chemistry ; Homeostasis ; In Situ Hybridization, Fluorescence ; Promoter Regions, Genetic ; RNA, Messenger/metabolism ; Regression Analysis ; Repressor Proteins ; Saccharomyces cerevisiae Proteins |
مستخلص: | Biosynthesis scales with cell size such that protein concentrations generally remain constant as cells grow. As an exception, synthesis of the cell-cycle inhibitor Whi5 "sub-scales" with cell size so that its concentration is lower in larger cells to promote cell-cycle entry. Here, we find that transcriptional control uncouples Whi5 synthesis from cell size, and we identify histones as the major class of sub-scaling transcripts besides WHI5 by screening for similar genes. Histone synthesis is thereby matched to genome content rather than cell size. Such sub-scaling proteins are challenged by asymmetric cell division because proteins are typically partitioned in proportion to newborn cell volume. To avoid this fate, Whi5 uses chromatin-binding to partition similar protein amounts to each newborn cell regardless of cell size. Disrupting both Whi5 synthesis and chromatin-based partitioning weakens G1 size control. Thus, specific transcriptional and partitioning mechanisms determine protein sub-scaling to control cell size. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
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معلومات مُعتمدة: | R35 GM134858 United States GM NIGMS NIH HHS; United States HHMI Howard Hughes Medical Institute |
فهرسة مساهمة: | Keywords: cell cycle; cell size; cell size control; gene expression; scaling |
المشرفين على المادة: | 0 (Chromatin) 0 (Histones) 0 (RNA, Messenger) 0 (Repressor Proteins) 0 (Saccharomyces cerevisiae Proteins) 0 (Whi5 protein, S cerevisiae) |
تواريخ الأحداث: | Date Created: 20211103 Date Completed: 20220107 Latest Revision: 20240404 |
رمز التحديث: | 20240404 |
مُعرف محوري في PubMed: | PMC8642314 |
DOI: | 10.1016/j.molcel.2021.10.007 |
PMID: | 34731644 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1097-4164 |
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DOI: | 10.1016/j.molcel.2021.10.007 |