دورية أكاديمية
أعرض في EDS

Transcriptional and chromatin-based partitioning mechanisms uncouple protein scaling from cell size.

التفاصيل البيبلوغرافية
العنوان: Transcriptional and chromatin-based partitioning mechanisms uncouple protein scaling from cell size.
المؤلفون: Swaffer MP; Department of Biology, Stanford University, Stanford, CA 94305, USA., Kim J; Department of Biology, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA., Chandler-Brown D; Department of Biology, Stanford University, Stanford, CA 94305, USA., Langhinrichs M; Department of Biology, Stanford University, Stanford, CA 94305, USA., Marinov GK; Department of Genetics, Stanford University, Stanford, CA 94305, USA., Greenleaf WJ; Department of Genetics, Stanford University, Stanford, CA 94305, USA., Kundaje A; Department of Genetics, Stanford University, Stanford, CA 94305, USA., Schmoller KM; Department of Biology, Stanford University, Stanford, CA 94305, USA; Institute of Functional Epigenetics, Helmholtz Zentrum München, 85764 Neuherberg, Germany., Skotheim JM; Department of Biology, Stanford University, Stanford, CA 94305, USA. Electronic address: skotheim@stanford.edu.
المصدر: Molecular cell [Mol Cell] 2021 Dec 02; Vol. 81 (23), pp. 4861-4875.e7. Date of Electronic Publication: 2021 Nov 02.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 9802571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4164 (Electronic) Linking ISSN: 10972765 NLM ISO Abbreviation: Mol Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge Ma : Cell Press
Original Publication: Cambridge, Mass. : Cell Press, c1997-
مواضيع طبية MeSH: Gene Expression Regulation, Fungal* , Transcription, Genetic*, Chromatin/*chemistry , Saccharomyces cerevisiae/*metabolism , Schizosaccharomyces/*metabolism, Cell Cycle ; Chromatin/metabolism ; Computational Biology ; Histones/chemistry ; Homeostasis ; In Situ Hybridization, Fluorescence ; Promoter Regions, Genetic ; RNA, Messenger/metabolism ; Regression Analysis ; Repressor Proteins ; Saccharomyces cerevisiae Proteins
مستخلص: Biosynthesis scales with cell size such that protein concentrations generally remain constant as cells grow. As an exception, synthesis of the cell-cycle inhibitor Whi5 "sub-scales" with cell size so that its concentration is lower in larger cells to promote cell-cycle entry. Here, we find that transcriptional control uncouples Whi5 synthesis from cell size, and we identify histones as the major class of sub-scaling transcripts besides WHI5 by screening for similar genes. Histone synthesis is thereby matched to genome content rather than cell size. Such sub-scaling proteins are challenged by asymmetric cell division because proteins are typically partitioned in proportion to newborn cell volume. To avoid this fate, Whi5 uses chromatin-binding to partition similar protein amounts to each newborn cell regardless of cell size. Disrupting both Whi5 synthesis and chromatin-based partitioning weakens G1 size control. Thus, specific transcriptional and partitioning mechanisms determine protein sub-scaling to control cell size.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: R35 GM134858 United States GM NIGMS NIH HHS; United States HHMI Howard Hughes Medical Institute
فهرسة مساهمة: Keywords: cell cycle; cell size; cell size control; gene expression; scaling
المشرفين على المادة: 0 (Chromatin)
0 (Histones)
0 (RNA, Messenger)
0 (Repressor Proteins)
0 (Saccharomyces cerevisiae Proteins)
0 (Whi5 protein, S cerevisiae)
تواريخ الأحداث: Date Created: 20211103 Date Completed: 20220107 Latest Revision: 20240404
رمز التحديث: 20240404
مُعرف محوري في PubMed: PMC8642314
DOI: 10.1016/j.molcel.2021.10.007
PMID: 34731644
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4164
DOI:10.1016/j.molcel.2021.10.007