دورية أكاديمية
The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers.
| العنوان: | The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers. |
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| المؤلفون: | Nakamoto R; Infectious Diseases Translational Research Programme, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545., Kwan JMC; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 308232., Chin JFL; Mechanobiology Institute, National University of Singapore 117411, Singapore., Ong HT; Mechanobiology Institute, National University of Singapore 117411, Singapore., Flores-Kim J; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115., Midonet C; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115., VanNieuwenhze MS; Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405., Guan XL; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 308232., Sham LT; Infectious Diseases Translational Research Programme, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545; lsham@nus.edu.sg. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Nov 09; Vol. 118 (45). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Bacterial Capsules/*metabolism , Bacterial Proteins/*metabolism , Galactosyltransferases/*metabolism , Polysaccharides, Bacterial/*metabolism , Protein-Tyrosine Kinases/*metabolism , Streptococcus pneumoniae/*enzymology, Bacterial Proteins/genetics ; Galactosyltransferases/genetics ; Streptococcus pneumoniae/genetics ; Streptococcus pneumoniae/growth & development |
| مستخلص: | Many pathogenic bacteria are encased in a layer of capsular polysaccharide (CPS). This layer is important for virulence by masking surface antigens, preventing opsonophagocytosis, and avoiding mucus entrapment. The bacterial tyrosine kinase (BY-kinase) regulates capsule synthesis and helps bacterial pathogens to survive different host niches. BY-kinases autophosphorylate at the C-terminal tyrosine residues upon external stimuli, but the role of phosphorylation is still unclear. Here, we report that the BY-kinase CpsCD is required for growth in Streptococcus pneumoniae Cells lacking a functional cpsC or cpsD accumulated low molecular weight CPS and lysed because of the lethal sequestration of the lipid carrier undecaprenyl phosphate, resulting in inhibition of peptidoglycan (PG) synthesis. CpsC interacts with CpsD and the polymerase CpsH. CpsD phosphorylation reduces the length of CPS polymers presumably by controlling the activity of CpsC. Finally, pulse-chase experiments reveal the spatiotemporal coordination between CPS and PG synthesis. This coordination is dependent on CpsC and CpsD. Together, our study provides evidence that BY-kinases regulate capsule polymer length by fine-tuning CpsC activity through autophosphorylation. Competing Interests: The authors declare no competing interest. |
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| معلومات مُعتمدة: | R35 GM136365 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: Streptococcus pneumoniae; bacterial tyrosine kinase; capsular polysaccharide |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Polysaccharides, Bacterial) EC 2.4.1.- (CpsD protein, bacteria) EC 2.4.1.- (Galactosyltransferases) EC 2.7.1.- (CpsD protein, Streptococcus pneumoniae) EC 2.7.10.1 (Protein-Tyrosine Kinases) |
| تواريخ الأحداث: | Date Created: 20211104 Date Completed: 20211213 Latest Revision: 20220506 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8609450 |
| DOI: | 10.1073/pnas.2103377118 |
| PMID: | 34732571 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
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| DOI: | 10.1073/pnas.2103377118 |