دورية أكاديمية
The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers.
العنوان: | The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers. |
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المؤلفون: | Nakamoto R; Infectious Diseases Translational Research Programme, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545., Kwan JMC; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 308232., Chin JFL; Mechanobiology Institute, National University of Singapore 117411, Singapore., Ong HT; Mechanobiology Institute, National University of Singapore 117411, Singapore., Flores-Kim J; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115., Midonet C; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115., VanNieuwenhze MS; Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405., Guan XL; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 308232., Sham LT; Infectious Diseases Translational Research Programme, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545; lsham@nus.edu.sg. |
المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Nov 09; Vol. 118 (45). |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media |
اللغة: | English |
بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
مواضيع طبية MeSH: | Bacterial Capsules/*metabolism , Bacterial Proteins/*metabolism , Galactosyltransferases/*metabolism , Polysaccharides, Bacterial/*metabolism , Protein-Tyrosine Kinases/*metabolism , Streptococcus pneumoniae/*enzymology, Bacterial Proteins/genetics ; Galactosyltransferases/genetics ; Streptococcus pneumoniae/genetics ; Streptococcus pneumoniae/growth & development |
مستخلص: | Many pathogenic bacteria are encased in a layer of capsular polysaccharide (CPS). This layer is important for virulence by masking surface antigens, preventing opsonophagocytosis, and avoiding mucus entrapment. The bacterial tyrosine kinase (BY-kinase) regulates capsule synthesis and helps bacterial pathogens to survive different host niches. BY-kinases autophosphorylate at the C-terminal tyrosine residues upon external stimuli, but the role of phosphorylation is still unclear. Here, we report that the BY-kinase CpsCD is required for growth in Streptococcus pneumoniae Cells lacking a functional cpsC or cpsD accumulated low molecular weight CPS and lysed because of the lethal sequestration of the lipid carrier undecaprenyl phosphate, resulting in inhibition of peptidoglycan (PG) synthesis. CpsC interacts with CpsD and the polymerase CpsH. CpsD phosphorylation reduces the length of CPS polymers presumably by controlling the activity of CpsC. Finally, pulse-chase experiments reveal the spatiotemporal coordination between CPS and PG synthesis. This coordination is dependent on CpsC and CpsD. Together, our study provides evidence that BY-kinases regulate capsule polymer length by fine-tuning CpsC activity through autophosphorylation. Competing Interests: The authors declare no competing interest. |
References: | Mol Microbiol. 2016 Feb;99(4):767-77. (PMID: 26538279) PLoS One. 2014 Jun 24;9(6):e100510. (PMID: 24959661) Trends Neurosci. 2014 Apr;37(4):211-8. (PMID: 24636457) Microbiol Spectr. 2019 Mar;7(2):. (PMID: 30977464) Mol Microbiol. 2017 Sep;105(5):689-704. (PMID: 28605069) PLoS Genet. 2015 Sep 17;11(9):e1005518. (PMID: 26378458) J Bacteriol. 1987 Feb;169(2):856-63. (PMID: 2433267) J Infect Dis. 1998 Feb;177(2):368-77. (PMID: 9466523) Nat Chem Biol. 2017 Apr;13(4):396-401. (PMID: 28166208) Microb Genom. 2019 Jul;5(7):. (PMID: 31184299) J Biol Chem. 2006 May 19;281(20):14048-56. (PMID: 16565080) Mol Microbiol. 2014 Oct;94(1):21-40. (PMID: 25099088) J Biol Chem. 2014 May 30;289(22):15680-90. (PMID: 24753256) Clin Microbiol Rev. 2015 Jul;28(3):871-99. (PMID: 26085553) Can J Biochem Physiol. 1959 Aug;37(8):911-7. (PMID: 13671378) Nat Microbiol. 2019 Oct;4(10):1661-1670. (PMID: 31182798) Annu Rev Microbiol. 2011;65:563-81. (PMID: 21721938) Nat Commun. 2021 Jan 14;12(1):369. (PMID: 33446644) Infect Immun. 2010 Feb;78(2):704-15. (PMID: 19948837) Nat Commun. 2018 Aug 23;9(1):3398. (PMID: 30139996) J Bacteriol. 2003 Oct;185(20):6057-66. (PMID: 14526017) J Infect Dis. 2004 Jan 15;189(2):328-38. (PMID: 14722899) Proc Natl Acad Sci U S A. 2017 May 30;114(22):5695-5700. (PMID: 28495967) J Biol Chem. 2020 Jul 31;295(31):10593-10609. (PMID: 32424042) Proc Natl Acad Sci U S A. 2006 May 30;103(22):8505-10. (PMID: 16707578) Proc Natl Acad Sci U S A. 2019 Apr 2;116(14):7062-7070. (PMID: 30872471) Nat Microbiol. 2016 Dec 12;2:16237. (PMID: 27941863) Infect Immun. 2014 Feb;82(2):694-705. (PMID: 24478084) Nat Chem Biol. 2010 Jun;6(6):418-23. (PMID: 20418877) Methods Mol Biol. 2017;1537:233-247. (PMID: 27924598) J Biol Chem. 2015 Apr 10;290(15):9521-32. (PMID: 25666613) Sci Rep. 2016 Jul 28;6:30176. (PMID: 27465908) Microb Drug Resist. 2014 Jun;20(3):199-214. (PMID: 24799078) Nat Commun. 2016 Feb 29;7:10859. (PMID: 26924467) Mol Microbiol. 2017 Mar;103(6):931-957. (PMID: 28010038) Antimicrob Agents Chemother. 2015;59(6):3548-55. (PMID: 25845878) PLoS Pathog. 2020 Mar 18;16(3):e1008417. (PMID: 32187228) J Bacteriol. 2013 Dec;195(24):5469-78. (PMID: 24097952) Nat Microbiol. 2016 Jun 20;1(7):16077. (PMID: 27572972) J Bacteriol. 2007 May;189(9):3369-81. (PMID: 17322316) Elife. 2019 Apr 09;8:. (PMID: 30964003) J Immunol. 1986 Jan;136(2):710-5. (PMID: 2416822) Infect Immun. 2001 Jun;69(6):3755-61. (PMID: 11349040) Appl Environ Microbiol. 2001 Nov;67(11):5190-6. (PMID: 11679344) Infect Immun. 2011 Jun;79(6):2314-23. (PMID: 21422174) Mucosal Immunol. 2017 Mar;10(2):385-394. (PMID: 27579859) J Bacteriol. 2014 Jun;196(11):1992-2003. (PMID: 24659769) Nat Commun. 2019 Mar 29;10(1):1404. (PMID: 30926919) EMBO J. 2011 Sep 30;30(24):4931-41. (PMID: 21964069) Clin Microbiol Infect. 2012 Oct;18 Suppl 5:15-24. (PMID: 22882735) Lancet Infect Dis. 2018 Nov;18(11):1191-1210. (PMID: 30243584) J Biol Chem. 2004 Jul 16;279(29):30106-13. (PMID: 15138271) Chem Sci. 2017 Sep 1;8(9):6313-6321. (PMID: 28989665) Microbiology (Reading). 2008 Dec;154(Pt 12):3877-3886. (PMID: 19047754) Nat Methods. 2009 Oct;6(10):767-72. (PMID: 19767758) Mol Microbiol. 2011 Oct;82(2):515-34. (PMID: 21929561) Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):3211-3220. (PMID: 30718427) Nat Commun. 2014 Sep 30;5:5055. (PMID: 25268848) Nature. 2016 Sep 29;537(7622):634-638. (PMID: 27525505) ACS Chem Biol. 2012 Oct 19;7(10):1746-53. (PMID: 22909777) FEMS Microbiol Rev. 2021 Aug 17;45(4):. (PMID: 33338218) J Bacteriol. 2005 Nov;187(21):7425-33. (PMID: 16237026) Proc Natl Acad Sci U S A. 2018 Jun 26;115(26):6709-6714. (PMID: 29891673) Science. 2011 Jan 28;331(6016):430-4. (PMID: 21273480) Microbiology (Reading). 2002 Jul;148(Pt 7):2045-2053. (PMID: 12101293) PLoS Biol. 2008 Jun 10;6(6):e143. (PMID: 18547145) Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):E848-57. (PMID: 23401533) |
معلومات مُعتمدة: | R35 GM136365 United States GM NIGMS NIH HHS |
فهرسة مساهمة: | Keywords: Streptococcus pneumoniae; bacterial tyrosine kinase; capsular polysaccharide |
المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Polysaccharides, Bacterial) EC 2.4.1.- (CpsD protein, bacteria) EC 2.4.1.- (Galactosyltransferases) EC 2.7.1.- (CpsD protein, Streptococcus pneumoniae) EC 2.7.10.1 (Protein-Tyrosine Kinases) |
تواريخ الأحداث: | Date Created: 20211104 Date Completed: 20211213 Latest Revision: 20220506 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8609450 |
DOI: | 10.1073/pnas.2103377118 |
PMID: | 34732571 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1091-6490 |
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DOI: | 10.1073/pnas.2103377118 |