دورية أكاديمية
أعرض في EDS

Basal and IL-1β enhanced chondrocyte chemotactic activity on monocytes are co-dependent on both IKKα and IKKβ NF-κB activating kinases.

التفاصيل البيبلوغرافية
العنوان: Basal and IL-1β enhanced chondrocyte chemotactic activity on monocytes are co-dependent on both IKKα and IKKβ NF-κB activating kinases.
المؤلفون: Olivotto E; Laboratorio RAMSES, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy., Minguzzi M; Dipartimento di Scienze Mediche e Chirurgiche (DIMEC), Università di Bologna, Bologna, Italy., D'Adamo S; Dipartimento di Scienze Mediche e Chirurgiche (DIMEC), Università di Bologna, Bologna, Italy., Astolfi A; Department of Translational Medicine, University of Ferrara, Ferrara, Italy., Santi S; Institute of Molecular Genetics 'Luigi Luca Cavalli-Sforza', Unit of Bologna, CNR, Bologna, Italy.; IRCCS, Istituto Ortopedico Rizzoli, Bologna, Italy., Uguccioni M; Institute for Research in Biomedicine, Università della Svizzera Italiana, Bellinzona, Switzerland.; Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy., Marcu KB; Departments of Biochemistry and Cell Biology and Pathology, SUNY, Stony Brook, NY, USA., Borzì RM; Laboratorio di Immunoreumatologia e Rigenerazione Tissutale, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy. rosamaria.borzi@ior.it.
المصدر: Scientific reports [Sci Rep] 2021 Nov 04; Vol. 11 (1), pp. 21697. Date of Electronic Publication: 2021 Nov 04.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: I-kappa B Kinase/*metabolism , Interleukin-1beta/*metabolism , Monocytes/*metabolism, Cells, Cultured ; Chemokine CCL2/metabolism ; Chemokines/immunology ; Chemokines/metabolism ; Chemotaxis/physiology ; Chondrocytes/metabolism ; Female ; Humans ; I-kappa B Kinase/physiology ; Inflammation ; Interleukin-1beta/physiology ; Male ; Middle Aged ; NF-kappa B/metabolism ; Osteoarthritis/metabolism ; Phosphorylation ; Protein Serine-Threonine Kinases ; Signal Transduction/physiology ; Transcription Factor RelA
مستخلص: IKKα and IKKβ are essential kinases for activating NF-κB transcription factors that regulate cellular differentiation and inflammation. By virtue of their small size, chemokines support the crosstalk between cartilage and other joint compartments and contribute to immune cell chemotaxis in osteoarthritis (OA). Here we employed shRNA retroviruses to stably and efficiently ablate the expression of each IKK in primary OA chondrocytes to determine their individual contributions for monocyte chemotaxis in response to chondrocyte conditioned media. Both IKKα and IKKβ KDs blunted both the monocyte chemotactic potential and the protein levels of CCL2/MCP-1, the chemokine with the highest concentration and the strongest association with monocyte chemotaxis. These findings were mirrored by gene expression analysis indicating that the lowest levels of CCL2/MCP-1 and other monocyte-active chemokines were in IKKαKD cells under both basal and IL-1β stimulated conditions. We find that in their response to IL-1β stimulation IKKαKD primary OA chondrocytes have reduced levels of phosphorylated NFkappaB p65pSer536 and H3pSer10. Confocal microscopy analysis revealed co-localized p65 and H3pSer10 nuclear signals in agreement with our findings that IKKαKD effectively blunts their basal level and IL-1β dependent increases. Our results suggest that IKKα could be a novel OA disease target.
(© 2021. The Author(s).)
References: Sci Rep. 2021 Jan 13;11(1):1053. (PMID: 33441764)
Arthritis Res Ther. 2010;12(1):R18. (PMID: 20113495)
Osteoarthritis Cartilage. 2017 Mar;25(3):406-412. (PMID: 27746376)
Nat Immunol. 2002 Jan;3(1):69-75. (PMID: 11743587)
J Cell Biochem. 2008 Jul 1;104(4):1393-406. (PMID: 18286508)
Eur Cell Mater. 2011 Feb 24;21:202-20. (PMID: 21351054)
Cell Signal. 2019 Jan;53:212-223. (PMID: 30312659)
Cold Spring Harb Perspect Biol. 2009 Oct;1(4):a000067. (PMID: 20066093)
J Biol Chem. 2006 Jul 7;281(27):18684-90. (PMID: 16675465)
Nature. 2001 Apr 5;410(6829):710-4. (PMID: 11287960)
J Cell Sci. 1988 Mar;89 ( Pt 3):373-8. (PMID: 3058725)
J Neuroinflammation. 2021 Mar 23;18(1):79. (PMID: 33757529)
Curr Drug Targets. 2007 Feb;8(2):367-76. (PMID: 17305514)
J Orthop Surg Res. 2020 Nov 10;15(1):516. (PMID: 33168099)
J Biol Chem. 2007 Jul 20;282(29):21308-18. (PMID: 17537731)
J Cell Physiol. 2007 Feb;210(2):417-27. (PMID: 17096385)
Osteoarthritis Cartilage. 2018 Sep;26(9):1257-1261. (PMID: 29723633)
Curr Drug Targets. 2007 Feb;8(2):377-85. (PMID: 17305515)
Oncogene. 2008 Oct 2;27(44):5833-44. (PMID: 18560356)
Genes Dis. 2015 Dec;2(4):307-327. (PMID: 26835506)
Arthritis Rheum. 2008 Jan;58(1):227-39. (PMID: 18163512)
Cell Rep. 2012 Oct 25;2(4):824-39. (PMID: 23063365)
Curr Opin Rheumatol. 2011 Sep;23(5):471-8. (PMID: 21788902)
J Cell Biochem. 2006 Jan 1;97(1):33-44. (PMID: 16215986)
Ann Rheum Dis. 2017 May;76(5):914-922. (PMID: 27965260)
Sci Rep. 2019 Jun 20;9(1):8905. (PMID: 31222033)
PLoS One. 2010 Feb 25;5(2):e9428. (PMID: 20195534)
J Orthop Res. 2017 Apr;35(4):735-739. (PMID: 27808445)
Nature. 2004 Apr 8;428(6983):660-4. (PMID: 15071597)
Arthritis Rheum. 2010 Aug;62(8):2370-81. (PMID: 20506238)
Ageing Res Rev. 2008 Apr;7(2):106-13. (PMID: 18054526)
Methods Mol Biol. 2012;806:301-36. (PMID: 22057461)
Ageing Res Rev. 2016 Aug;29:50-65. (PMID: 27328278)
Immunol Rev. 2012 Mar;246(1):239-53. (PMID: 22435559)
Osteoarthritis Cartilage. 2017 Apr;25(4):589-599. (PMID: 27836674)
Arthritis Res Ther. 2019 Jun 13;21(1):146. (PMID: 31196179)
Mol Cell. 2004 Oct 22;16(2):245-55. (PMID: 15494311)
Nat Rev Rheumatol. 2012 Sep;8(9):543-52. (PMID: 22890245)
PLoS One. 2013 Sep 02;8(9):e73024. (PMID: 24023802)
Methods Mol Biol. 2004;285:171-7. (PMID: 15269412)
Oxid Med Cell Longev. 2018 Feb 11;2018:3075293. (PMID: 29599894)
Ther Adv Musculoskelet Dis. 2013 Apr;5(2):77-94. (PMID: 23641259)
J Gerontol A Biol Sci Med Sci. 2013 Oct;68(10):1209-17. (PMID: 23689830)
Arthritis Rheum. 2008 Jul;58(7):2075-87. (PMID: 18576389)
Biosci Rep. 2016 May 06;36(3):. (PMID: 27129293)
Free Radic Biol Med. 2021 Apr;166:212-225. (PMID: 33636333)
Science. 1999 Apr 9;284(5412):316-20. (PMID: 10195896)
J Immunol. 2018 Oct 1;201(7):1918-1927. (PMID: 30135182)
Trends Cell Biol. 2021 Mar;31(3):166-178. (PMID: 33422358)
Nature. 2003 Jun 5;423(6940):655-9. (PMID: 12789342)
EMBO Rep. 2014 Jan;15(1):46-61. (PMID: 24375677)
Nat Rev Rheumatol. 2016 Nov;12(11):669-683. (PMID: 27733758)
Nature. 2003 Jun 5;423(6940):659-63. (PMID: 12789343)
Clin Rheumatol. 2008 Sep;27(9):1127-34. (PMID: 18414968)
Clin Exp Rheumatol. 2019 Sep-Oct;37 Suppl 120(5):57-63. (PMID: 31621560)
J Cell Sci. 2003 Sep 15;116(Pt 18):3677-85. (PMID: 12917355)
Ann Rheum Dis. 2005 Sep;64(9):1263-7. (PMID: 15731292)
J Biol Chem. 2002 Nov 22;277(47):45129-40. (PMID: 12221085)
Osteoarthritis Cartilage. 2007 Sep;15(9):1061-9. (PMID: 17470400)
Cell. 1997 Oct 17;91(2):243-52. (PMID: 9346241)
Curr Rheumatol Rep. 2017 Mar;19(3):11. (PMID: 28265846)
J Clin Invest. 2001 Jan;107(2):135-42. (PMID: 11160126)
Curr Drug Targets. 2010 May;11(5):599-613. (PMID: 20199390)
Cell. 2007 Sep 7;130(5):918-31. (PMID: 17803913)
Arthritis Rheumatol. 2018 Feb;70(2):185-192. (PMID: 29178176)
Sci Rep. 2019 Sep 19;9(1):13603. (PMID: 31537813)
Pharm Res. 2018 Apr 20;35(6):123. (PMID: 29679159)
Osteoarthritis Cartilage. 2020 May;28(5):613-625. (PMID: 32006659)
Nat Rev Rheumatol. 2010 Nov;6(11):625-35. (PMID: 20924410)
Signal Transduct Target Ther. 2017;2:. (PMID: 29158945)
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20798-803. (PMID: 19104039)
Sci Rep. 2019 Oct 3;9(1):14269. (PMID: 31582764)
معلومات مُعتمدة: FIRB Grant: RBAP10KCNS Ministero dell'Istruzione, dell'Università e della Ricerca, Italy; Fondi Cinque per Mille to the IRCCS Istituto Ortopedico Rizzoli Ministero della Salute, Italy
المشرفين على المادة: 0 (CCL2 protein, human)
0 (Chemokine CCL2)
0 (Chemokines)
0 (IL1B protein, human)
0 (Interleukin-1beta)
0 (NF-kappa B)
0 (RELA protein, human)
0 (Transcription Factor RelA)
EC 2.7.11.1 (Protein Serine-Threonine Kinases)
EC 2.7.11.1 (TBK1 protein, human)
EC 2.7.11.10 (I-kappa B Kinase)
تواريخ الأحداث: Date Created: 20211105 Date Completed: 20220125 Latest Revision: 20220125
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8568921
DOI: 10.1038/s41598-021-01063-2
PMID: 34737366
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-021-01063-2