دورية أكاديمية
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Purification and characterization of a highly thermostable GlcNAc-binding lectin from Collaea speciosa seeds.

التفاصيل البيبلوغرافية
العنوان: Purification and characterization of a highly thermostable GlcNAc-binding lectin from Collaea speciosa seeds.
المؤلفون: Oliveira MV; BioMol-Lab, Campus do Pici, Universidade Federal do Ceará, Fortaleza, Ceará 60.440-970, Brazil., Osterne VJS; BioMol-Lab, Campus do Pici, Universidade Federal do Ceará, Fortaleza, Ceará 60.440-970, Brazil; Laboratory for Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, 9000 Ghent, Belgium., Lossio CF; BioMol-Lab, Campus do Pici, Universidade Federal do Ceará, Fortaleza, Ceará 60.440-970, Brazil., Serna S; Glycotechnology Laboratory, Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo Miramón 182, 20014 San Sebastian, Spain., Reichardt NC; Glycotechnology Laboratory, Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo Miramón 182, 20014 San Sebastian, Spain; CIBER-BBN, Paseo Miramón 182, 20009 San Sebastian, Spain., Nascimento KS; BioMol-Lab, Campus do Pici, Universidade Federal do Ceará, Fortaleza, Ceará 60.440-970, Brazil., Van Damme EJM; Laboratory for Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, 9000 Ghent, Belgium. Electronic address: ElsJM.VanDamme@ugent.be., Cavada BS; BioMol-Lab, Campus do Pici, Universidade Federal do Ceará, Fortaleza, Ceará 60.440-970, Brazil. Electronic address: bscavada@ufc.br.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2021 Dec 15; Vol. 193 (Pt B), pp. 1562-1571. Date of Electronic Publication: 2021 Nov 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Fabaceae/*chemistry , Plant Lectins/*chemistry , Polysaccharides/*chemistry , Polysaccharides/*metabolism , Seeds/*chemistry, Amino Acid Sequence ; Animals ; Artemia/metabolism ; Glucose/metabolism ; Hemagglutination ; Mannose/metabolism
مستخلص: Lectins from plants of the Diocleinae subtribe often exhibit specificity towards mannose/glucose and derived sugars, with some plants also displaying a second lectin specific to lactose/GalNAc. Here, we present a novel lectin from Collaea speciosa, named CsL, that displays specificity for GlcNAc/glucose. The lectin was extracted from Collaea speciosa seeds and purified by a single chromatographic step on a Sephadex G-50 matrix. In solution, the lectin appears as a dimeric protein composed of 25 kDa monomers. The protein is stable at pH 7-8 and dependent on divalent cations. CsL maintained its agglutination activity after heating to 90 °C for 1 h. Glycan array studies revealed that CsL binds to N-glycans with terminal GlcNAc residues, chitobiose and chitotriose moieties. The partial amino acid sequence of the lectin is similar to that of some lactose-specific lectins from the same subtribe. In contrast to other ConA-like lectins, CsL is not toxic to Artemia. Because of its remarkably different properties and specificity, this lectin could be the first member of a new group inside the Diocleinae lectins.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Biological properties; Collaea speciosa; Lectin
المشرفين على المادة: 0 (Plant Lectins)
0 (Polysaccharides)
IY9XDZ35W2 (Glucose)
PHA4727WTP (Mannose)
تواريخ الأحداث: Date Created: 20211106 Date Completed: 20211223 Latest Revision: 20211223
رمز التحديث: 20231215
DOI: 10.1016/j.ijbiomac.2021.10.219
PMID: 34740693
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2021.10.219