دورية أكاديمية
Proteome characterization of the culture supernatant of Mycobacterium bovis in different growth stages.
العنوان: | Proteome characterization of the culture supernatant of Mycobacterium bovis in different growth stages. |
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المؤلفون: | Assal N; Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada.; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada., Rennie B; Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada., Walrond L; Centre for Vaccine Evaluation, Health Canada, Ottawa, ON, Canada., Cyr T; Centre for Vaccine Evaluation, Health Canada, Ottawa, ON, Canada., Rohonczy L; Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada., Lin M; Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada.; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada. |
المصدر: | Biochemistry and biophysics reports [Biochem Biophys Rep] 2021 Oct 23; Vol. 28, pp. 101154. Date of Electronic Publication: 2021 Oct 23 (Print Publication: 2021). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101660999 Publication Model: eCollection Cited Medium: Internet ISSN: 2405-5808 (Electronic) Linking ISSN: 24055808 NLM ISO Abbreviation: Biochem Biophys Rep Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier B.V., [2015]- |
مستخلص: | This study aimed to identify proteins secreted by Mycobacterium bovis into culture medium at different stages of bacterial growth. A field strain of M. bovis was grown in Middlebrook 7H9 media and culture supernatant was collected at three-time points representing three different phases of growth (early exponential, late exponential, and stationary phases). Supernatants were double filtered, digested by trypsin and analyzed by LC-MS/MS. The study found 15, 21, and 16 proteins in early, mid and late growth phases, respectively. In total, 22 proteins were identified, 18 of which were reported or predicted to have a cell wall or extracellular localization. To our knowledge, this is the first study to identify proteins secreted into the culture medium by a field strain of M. bovis in three different stages of growth. The dataset generated here provides candidate proteins with the potential for the development of serological diagnostic reagents or vaccine for bovine tuberculosis. Data are available via ProteomeXchange with identifier PXD017817. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Crown Copyright © 2021 Published by Elsevier B.V.) |
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فهرسة مساهمة: | Keywords: Culture supernatant; Growth stages; Mycobacterium bovis; Protein antigens; Proteomics |
تواريخ الأحداث: | Date Created: 20211108 Latest Revision: 20211110 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8554454 |
DOI: | 10.1016/j.bbrep.2021.101154 |
PMID: | 34746449 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2405-5808 |
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DOI: | 10.1016/j.bbrep.2021.101154 |