دورية أكاديمية
أعرض في EDS

Proteome characterization of the culture supernatant of Mycobacterium bovis in different growth stages.

التفاصيل البيبلوغرافية
العنوان: Proteome characterization of the culture supernatant of Mycobacterium bovis in different growth stages.
المؤلفون: Assal N; Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada.; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada., Rennie B; Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada., Walrond L; Centre for Vaccine Evaluation, Health Canada, Ottawa, ON, Canada., Cyr T; Centre for Vaccine Evaluation, Health Canada, Ottawa, ON, Canada., Rohonczy L; Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada., Lin M; Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada.; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
المصدر: Biochemistry and biophysics reports [Biochem Biophys Rep] 2021 Oct 23; Vol. 28, pp. 101154. Date of Electronic Publication: 2021 Oct 23 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101660999 Publication Model: eCollection Cited Medium: Internet ISSN: 2405-5808 (Electronic) Linking ISSN: 24055808 NLM ISO Abbreviation: Biochem Biophys Rep Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier B.V., [2015]-
مستخلص: This study aimed to identify proteins secreted by Mycobacterium bovis into culture medium at different stages of bacterial growth. A field strain of M. bovis was grown in Middlebrook 7H9 media and culture supernatant was collected at three-time points representing three different phases of growth (early exponential, late exponential, and stationary phases). Supernatants were double filtered, digested by trypsin and analyzed by LC-MS/MS. The study found 15, 21, and 16 proteins in early, mid and late growth phases, respectively. In total, 22 proteins were identified, 18 of which were reported or predicted to have a cell wall or extracellular localization. To our knowledge, this is the first study to identify proteins secreted into the culture medium by a field strain of M. bovis in three different stages of growth. The dataset generated here provides candidate proteins with the potential for the development of serological diagnostic reagents or vaccine for bovine tuberculosis. Data are available via ProteomeXchange with identifier PXD017817.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Crown Copyright © 2021 Published by Elsevier B.V.)
References: Trends Microbiol. 2005 May;13(5):206-11. (PMID: 15866037)
Braz J Microbiol. 2013 May 31;44(1):287-9. (PMID: 24159318)
Infect Immun. 1998 Sep;66(9):4208-14. (PMID: 9712769)
Tuberculosis (Edinb). 2009 Nov;89(6):423-30. (PMID: 19683472)
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450. (PMID: 30395289)
EMBO J. 2006 Apr 5;25(7):1436-44. (PMID: 16541102)
J Proteomics. 2014 Jan 31;97:141-50. (PMID: 23891556)
J Clin Microbiol. 1998 Sep;36(9):2752-4. (PMID: 9705430)
Genome Announc. 2017 Apr 6;5(14):. (PMID: 28385856)
Biochim Biophys Acta Gen Subj. 2019 Sep;1863(9):1351-1359. (PMID: 31175911)
J Clin Microbiol. 2003 Aug;41(8):3929-32. (PMID: 12904421)
BMC Vet Res. 2010 Nov 09;6:50. (PMID: 21062483)
Infect Immun. 1998 Feb;66(2):794-9. (PMID: 9453643)
Microbiol Res. 2014 Sep-Oct;169(9-10):780-7. (PMID: 24556072)
Proteomics. 2007 May;7(10):1702-18. (PMID: 17443846)
Mol Biol Cell. 2005 Jan;16(1):396-404. (PMID: 15525680)
Infect Immun. 1992 May;60(5):2066-74. (PMID: 1563797)
Tuber Lung Dis. 1995 Aug;76 Suppl 1:1-46. (PMID: 7579326)
mSphere. 2019 Jun 5;4(3):. (PMID: 31167949)
Vet Microbiol. 2018 Feb;214:44-50. (PMID: 29408031)
Vet Microbiol. 1994 May;40(1-2):111-24. (PMID: 8073619)
Clin Diagn Lab Immunol. 2001 May;8(3):571-8. (PMID: 11329460)
J Biol Chem. 2014 Sep 5;289(36):25041-53. (PMID: 25028517)
Proteomics. 2012 Apr;12(7):979-91. (PMID: 22522804)
Res Vet Sci. 1990 Jul;49(1):46-9. (PMID: 2116655)
Infect Immun. 2009 Aug;77(8):3389-401. (PMID: 19470749)
Microorganisms. 2021 Aug 23;9(8):. (PMID: 34442871)
J Transl Med. 2017 Apr 3;15(1):68. (PMID: 28372590)
Res Vet Sci. 2015 Aug;101:117-9. (PMID: 26267100)
Biochem Biophys Res Commun. 2010 Nov 26;402(4):693-8. (PMID: 20977881)
Acta Trop. 2011 May;118(2):101-4. (PMID: 21420374)
Indian J Med Res. 2010 Oct;132:400-8. (PMID: 20966518)
Brief Bioinform. 2011 Sep;12(5):449-62. (PMID: 21873635)
فهرسة مساهمة: Keywords: Culture supernatant; Growth stages; Mycobacterium bovis; Protein antigens; Proteomics
تواريخ الأحداث: Date Created: 20211108 Latest Revision: 20211110
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8554454
DOI: 10.1016/j.bbrep.2021.101154
PMID: 34746449
قاعدة البيانات: MEDLINE
الوصف
تدمد:2405-5808
DOI:10.1016/j.bbrep.2021.101154