دورية أكاديمية
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Proximity-Labeling Reveals Novel Host and Parasite Proteins at the Toxoplasma Parasitophorous Vacuole Membrane.

التفاصيل البيبلوغرافية
العنوان: Proximity-Labeling Reveals Novel Host and Parasite Proteins at the Toxoplasma Parasitophorous Vacuole Membrane.
المؤلفون: Cygan AM; Department of Microbiology and Immunology, Stanford School of Medicine, Stanford California, USA., Jean Beltran PM; Broad Institutegrid.66859.34 of MIT and Harvard, Cambridge, Massachusetts, USA., Mendoza AG; Department of Microbiology and Immunology, Stanford School of Medicine, Stanford California, USA., Branon TC; Department of Chemistry, Stanford Universitygrid.168010.e, Stanford, California, USA., Ting AY; Department of Chemistry, Stanford Universitygrid.168010.e, Stanford, California, USA., Carr SA; Broad Institutegrid.66859.34 of MIT and Harvard, Cambridge, Massachusetts, USA., Boothroyd JC; Department of Microbiology and Immunology, Stanford School of Medicine, Stanford California, USA.
المصدر: MBio [mBio] 2021 Dec 21; Vol. 12 (6), pp. e0026021. Date of Electronic Publication: 2021 Nov 09.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101519231 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2150-7511 (Electronic) NLM ISO Abbreviation: mBio Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Society for Microbiology
مواضيع طبية MeSH: Intracellular Membranes/*metabolism , Intracellular Membranes/*parasitology , Protozoan Proteins/*metabolism , Toxoplasma/*metabolism , Toxoplasmosis/*metabolism , Toxoplasmosis/*parasitology , Vacuoles/*parasitology, Apoptosis Regulatory Proteins/genetics ; Apoptosis Regulatory Proteins/metabolism ; Calcium-Binding Proteins/genetics ; Calcium-Binding Proteins/metabolism ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Endosomal Sorting Complexes Required for Transport/genetics ; Endosomal Sorting Complexes Required for Transport/metabolism ; Host-Parasite Interactions ; Humans ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Protein Transport ; Protozoan Proteins/genetics ; Receptors, Chemokine/genetics ; Receptors, Chemokine/metabolism ; Toxoplasma/genetics ; Toxoplasmosis/genetics ; Vacuoles/genetics ; Vacuoles/metabolism
مستخلص: Toxoplasma gondii is a ubiquitous, intracellular parasite that envelops its parasitophorous vacuole with a protein-laden membrane (PVM). The PVM is critical for interactions with the infected host cell, such as nutrient transport and immune defense. Only a few parasite and host proteins have so far been identified on the host-cytosolic side of the Toxoplasma PVM. We report here the use of human foreskin fibroblasts expressing the proximity-labeling enzyme miniTurbo, fused to a domain that targets it to this face of the PVM, in combination with quantitative proteomics to specifically identify proteins present at this interface. Out of numerous human and parasite proteins with candidate PVM localization, we validate three parasite proteins (TGGT1_269950 [GRA61], TGGT1_215360 [GRA62], and TGGT1_217530 [GRA63]) and four new host proteins (PDCD6IP/ALIX, PDCD6, CC2D1A, and MOSPD2) as localized to the PVM in infected human cells through immunofluorescence microscopy. These results significantly expand our knowledge of proteins present at the Toxoplasma PVM and, given that three of the validated host proteins are components of the ESCRT (endosomal sorting complexes required for transport) machinery, they further suggest that novel biology is operating at this crucial host-pathogen interface. IMPORTANCE Toxoplasma is an intracellular pathogen which resides and replicates inside a membrane-bound vacuole in infected cells. This vacuole is modified by both parasite and host proteins which participate in a variety of host-parasite interactions at this interface, including nutrient exchange, effector transport, and immune modulation. Only a small number of parasite and host proteins present at the vacuolar membrane and exposed to the host cytosol have thus far been identified. Here, we report the identification of several novel parasite and host proteins present at the vacuolar membrane using enzyme-catalyzed proximity-labeling, significantly increasing our knowledge of the molecular players present and novel biology occurring at this crucial interface.
References: Cell Host Microbe. 2014 May 14;15(5):537-50. (PMID: 24832449)
mBio. 2020 Feb 4;11(1):. (PMID: 32019789)
Nat Commun. 2020 Oct 16;11(1):5258. (PMID: 33067458)
Int J Parasitol. 2009 Feb;39(3):299-306. (PMID: 18840447)
Traffic. 2009 Oct;10(10):1458-70. (PMID: 19682324)
Genome Res. 2003 Nov;13(11):2498-504. (PMID: 14597658)
Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):E2453-61. (PMID: 24927568)
Nat Microbiol. 2019 Nov;4(11):1951-1963. (PMID: 31332383)
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W429-32. (PMID: 17483518)
Infect Immun. 2008 Dec;76(12):5853-61. (PMID: 18809661)
Mol Biochem Parasitol. 1993 May;59(1):143-53. (PMID: 8515776)
J Cell Biol. 1997 Feb 10;136(3):531-44. (PMID: 9024685)
J Protozool. 1992 Jul-Aug;39(4):526-30. (PMID: 1403988)
Cell Host Microbe. 2020 Nov 11;28(5):752-766.e9. (PMID: 33053376)
Nat Rev Mol Cell Biol. 2020 Jan;21(1):25-42. (PMID: 31705132)
Immunology. 1998 Aug;94(4):488-95. (PMID: 9767436)
J Biol Chem. 2005 Oct 7;280(40):34245-58. (PMID: 16002398)
Science. 2001 Oct 5;294(5540):161-5. (PMID: 11588262)
Proc Natl Acad Sci U S A. 1984 Feb;81(3):908-12. (PMID: 6422465)
PLoS Pathog. 2021 Dec 13;17(12):e1010138. (PMID: 34898650)
J Cell Sci. 1995 Apr;108 ( Pt 4):1669-77. (PMID: 7615684)
Microbes Infect. 2008 Nov-Dec;10(14-15):1440-9. (PMID: 18983931)
Nat Commun. 2019 Sep 3;10(1):3963. (PMID: 31481656)
Biocell. 2001 Apr;25(1):53-9. (PMID: 11387877)
J Proteome Res. 2019 Feb 1;18(2):623-632. (PMID: 30450911)
Anal Chem. 2020 Jan 21;92(2):2005-2010. (PMID: 31869197)
Science. 2006 Dec 15;314(5806):1780-3. (PMID: 17170306)
J Cell Sci. 1997 Sep;110 ( Pt 17):2117-28. (PMID: 9378762)
Cell Host Microbe. 2013 Apr 17;13(4):489-500. (PMID: 23601110)
Clin Microbiol Rev. 2017 Jul;30(3):615-645. (PMID: 28404792)
Nucleic Acids Res. 2019 Jul 2;47(W1):W191-W198. (PMID: 31066453)
Mol Biochem Parasitol. 2013 May;189(1-2):5-13. (PMID: 23623919)
PLoS Pathog. 2009 Feb;5(2):e1000309. (PMID: 19247437)
PLoS Pathog. 2018 Jan 22;14(1):e1006828. (PMID: 29357375)
PLoS Biol. 2014 Apr 29;12(4):e1001845. (PMID: 24781109)
J Exp Med. 1972 Nov 1;136(5):1173-94. (PMID: 4343243)
EMBO J. 2001 Jun 15;20(12):3132-44. (PMID: 11406590)
Mol Biochem Parasitol. 2000 Jan 5;105(1):25-37. (PMID: 10613696)
Curr Opin Microbiol. 2019 Dec;52:130-138. (PMID: 31446366)
Eukaryot Cell. 2009 Apr;8(4):520-9. (PMID: 19218423)
Trends Parasitol. 2017 Jun;33(6):473-488. (PMID: 28330745)
J Mol Biol. 2012 May 25;419(1-2):75-88. (PMID: 22406677)
mSphere. 2019 Jul 31;4(4):. (PMID: 31366709)
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613. (PMID: 30476243)
mBio. 2016 Aug 02;7(4):. (PMID: 27486190)
EMBO Rep. 2018 Jul;19(7):. (PMID: 29858488)
Cell Microbiol. 2008 Feb;10(2):465-76. (PMID: 17970763)
J Cell Biol. 2000 Apr 3;149(1):167-80. (PMID: 10747095)
Elife. 2015 Nov 18;4:. (PMID: 26576949)
Mol Biol Cell. 1999 Apr;10(4):1277-87. (PMID: 10198072)
J Cell Biol. 2012 Mar 19;196(6):801-10. (PMID: 22412018)
J Proteome Res. 2019 Jan 4;18(1):319-330. (PMID: 30362762)
Nat Microbiol. 2017 Oct;2(10):1358-1366. (PMID: 28848228)
Anim Health Res Rev. 2005 Jun;6(1):41-61. (PMID: 16164008)
Trends Parasitol. 2002 Nov;18(11):482. (PMID: 12473362)
Trends Biochem Sci. 2017 Jan;42(1):42-56. (PMID: 27669649)
Infect Immun. 2014 Jun;82(6):2595-605. (PMID: 24711568)
mSphere. 2016 Mar 23;1(2):. (PMID: 27303719)
Int J Mol Sci. 2016 Aug 26;17(9):. (PMID: 27571067)
Nat Biotechnol. 2018 Oct;36(9):880-887. (PMID: 30125270)
mSphere. 2020 Jun 24;5(3):. (PMID: 32581080)
mBio. 2016 Feb 02;7(1):e02231-15. (PMID: 26838724)
Cell Microbiol. 2007 Jan;9(1):54-64. (PMID: 16879455)
Mol Biochem Parasitol. 1999 Aug 20;102(2):311-24. (PMID: 10498186)
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2723-8. (PMID: 11226307)
Cell. 2016 Sep 8;166(6):1423-1435.e12. (PMID: 27594426)
Cell Host Microbe. 2019 Oct 9;26(4):478-492.e8. (PMID: 31600500)
معلومات مُعتمدة: F32 HL154711 United States HL NHLBI NIH HHS; R01 AI021423 United States AI NIAID NIH HHS; R01 AI129529 United States AI NIAID NIH HHS; R01 DK121409 United States DK NIDDK NIH HHS
فهرسة مساهمة: Keywords: Toxoplasma; proximity-labeling; quantitative proteomics
المشرفين على المادة: 0 (Apoptosis Regulatory Proteins)
0 (CC2D1A protein, human)
0 (Calcium-Binding Proteins)
0 (Cell Cycle Proteins)
0 (DNA-Binding Proteins)
0 (Endosomal Sorting Complexes Required for Transport)
0 (MOSPD2 protein, human)
0 (Membrane Proteins)
0 (PDCD6 protein, human)
0 (PDCD6IP protein, human)
0 (Protozoan Proteins)
0 (Receptors, Chemokine)
تواريخ الأحداث: Date Created: 20211109 Date Completed: 20220222 Latest Revision: 20220302
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8576527
DOI: 10.1128/mBio.00260-21
PMID: 34749525
قاعدة البيانات: MEDLINE
الوصف
تدمد:2150-7511
DOI:10.1128/mBio.00260-21