Equipment-free detection of SARS-CoV-2 and Variants of Concern using Cas13.
العنوان: | Equipment-free detection of SARS-CoV-2 and Variants of Concern using Cas13. |
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المؤلفون: | Arizti-Sanz J; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard-MIT Program in Health Sciences and Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA., Bradley A; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Zhang YB; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA., Boehm CK; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA., Freije CA; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Grunberg ME; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA., Kosoko-Thoroddsen TF; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Welch NL; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Program in Virology, Harvard Medical School, Boston, MA 02115, USA., Pillai PP; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Mantena S; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Kim G; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA., Uwanibe JN; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., John OG; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., Eromon PE; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria., Kocher G; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and infectious diseases, National Institute of Health, Frederick, MD 21702, USA., Gross R; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and infectious diseases, National Institute of Health, Frederick, MD 21702, USA., Lee JS; Biotechnology Cores Facility Branch,Division of Scientific Resources, National Center for Emerging and Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Hensley LE; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and infectious diseases, National Institute of Health, Frederick, MD 21702, USA., Happi CT; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria.; Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, MA 02115, USA., Johnson J; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Sabeti PC; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA.; Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, MA 02115, USA.; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.; These authors jointly supervised this work: Pardis C. Sabeti, Cameron Myhrvold., Myhrvold C; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.; These authors jointly supervised this work: Pardis C. Sabeti, Cameron Myhrvold. |
المصدر: | MedRxiv : the preprint server for health sciences [medRxiv] 2021 Nov 02. Date of Electronic Publication: 2021 Nov 02. |
نوع المنشور: | Preprint |
اللغة: | English |
بيانات الدورية: | Country of Publication: United States NLM ID: 101767986 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: medRxiv Subsets: PubMed not MEDLINE |
مستخلص: | The COVID-19 pandemic, and the recent rise and widespread transmission of SARS-CoV-2 Variants of Concern (VOCs), have demonstrated the need for ubiquitous nucleic acid testing outside of centralized clinical laboratories. Here, we develop SHINEv2, a Cas13-based nucleic acid diagnostic that combines quick and ambient temperature sample processing and lyophilized reagents to greatly simplify the test procedure and assay distribution. We benchmarked a SHINEv2 assay for SARS-CoV-2 detection against state-of-the-art antigen-capture tests using 96 patient samples, demonstrating 50-fold greater sensitivity and 100% specificity. We designed SHINEv2 assays for discriminating the Alpha, Beta, Gamma and Delta VOCs, which can be read out visually using lateral flow technology. We further demonstrate that our assays can be performed without any equipment in less than 90 minutes. SHINEv2 represents an important advance towards rapid nucleic acid tests that can be performed in any location. |
التعليقات: | Update in: Nat Biomed Eng. 2022 Aug;6(8):932-943. (PMID: 35637389) |
References: | J Clin Microbiol. 2021 Apr 20;59(5):. (PMID: 33622768) Nat Commun. 2020 Nov 20;11(1):5920. (PMID: 33219228) Euro Surveill. 2021 Mar;26(10):. (PMID: 33706862) J Clin Microbiol. 2021 Sep 20;59(10):e0110721. (PMID: 34346715) Nat Chem Biol. 2021 Sep;17(9):982-988. (PMID: 34354262) Anal Chem. 2020 Mar 3;92(5):4029-4037. (PMID: 32031369) PLoS One. 2021 Dec 15;16(12):e0260487. (PMID: 34910739) Nat Microbiol. 2021 Jul;6(7):821-823. (PMID: 34108654) N Engl J Med. 2020 Oct 8;383(15):1492-1494. (PMID: 32937062) Sci Adv. 2021 Aug 6;7(32):. (PMID: 34362739) Proc Natl Acad Sci U S A. 2020 Sep 29;117(39):24450-24458. (PMID: 32900935) Glob Chall. 2017 Jan 10;1(1):33-46. (PMID: 31565258) Nat Commun. 2020 Nov 20;11(1):5921. (PMID: 33219225) Nature. 2021 May;593(7858):266-269. (PMID: 33767447) Nat Microbiol. 2019 Jan;4(1):46-54. (PMID: 30546093) Cell Discov. 2018 Apr 24;4:20. (PMID: 29707234) Nat Commun. 2021 Jan 11;12(1):267. (PMID: 33431879) Nat Commun. 2022 Nov 16;13(1):7003. (PMID: 36385137) Science. 2018 Apr 27;360(6387):436-439. (PMID: 29449511) Nat Commun. 2021 May 18;12(1):2905. (PMID: 34006857) Sci Transl Med. 2020 Aug 12;12(556):. (PMID: 32719001) Science. 2021 Jan 8;371(6525):126-127. (PMID: 33414210) Nat Rev Microbiol. 2021 Jul;19(7):409-424. (PMID: 34075212) Nature. 2020 Jun;582(7811):277-282. (PMID: 32349121) Science. 2018 Apr 27;360(6387):439-444. (PMID: 29449508) PLoS Biol. 2021 May 7;19(5):e3001236. (PMID: 33961632) MMWR Morb Mortal Wkly Rep. 2021 Jan 01;69(5152):1642-1647. (PMID: 33382679) PLoS Biol. 2021 Apr 29;19(4):e3001216. (PMID: 33914730) Emerg Infect Dis. 2020 Aug;26(8):1944-1946. (PMID: 32433015) Cell. 2021 Jan 21;184(2):323-333.e9. (PMID: 33306959) Science. 2021 May 28;372(6545):941-948. (PMID: 33906967) Proc Natl Acad Sci U S A. 2021 Aug 31;118(35):. (PMID: 34400545) Euro Surveill. 2017 Mar 30;22(13):. (PMID: 28382917) JAMA. 2020 May 19;323(19):1889-1890. (PMID: 32301959) Lancet Reg Health West Pac. 2020 Nov;4:100044. (PMID: 34013216) JAMA Netw Open. 2020 Jul 1;3(7):e2016818. (PMID: 32735339) Nature. 2021 Aug;596(7871):276-280. (PMID: 34237773) PLoS One. 2014 Nov 05;9(11):e112146. (PMID: 25372030) J Clin Microbiol. 2021 Feb 18;59(3):. (PMID: 33310764) Open Forum Infect Dis. 2021 May 26;8(7):ofab243. (PMID: 34250188) Ann Intern Med. 2021 Jul;174(7):945-951. (PMID: 33900791) Science. 2017 Apr 28;356(6336):438-442. (PMID: 28408723) Science. 2021 May 7;372(6542):635-641. (PMID: 33758017) J Clin Microbiol. 2021 Mar 19;59(4):. (PMID: 33509809) Science. 2021 Feb 5;371(6529):. (PMID: 33303686) Science. 2018 Apr 27;360(6387):444-448. (PMID: 29700266) Lancet. 2021 Apr 10;397(10282):1346-1347. (PMID: 33765408) |
تواريخ الأحداث: | Date Created: 20211109 Latest Revision: 20231108 |
رمز التحديث: | 20231108 |
مُعرف محوري في PubMed: | PMC8575147 |
DOI: | 10.1101/2021.11.01.21265764 |
PMID: | 34751276 |
قاعدة البيانات: | MEDLINE |
DOI: | 10.1101/2021.11.01.21265764 |
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