دورية أكاديمية
أعرض في EDS

Surface-tethered planar membranes containing the β-barrel assembly machinery: a platform for investigating bacterial outer membrane protein folding.

التفاصيل البيبلوغرافية
العنوان: Surface-tethered planar membranes containing the β-barrel assembly machinery: a platform for investigating bacterial outer membrane protein folding.
المؤلفون: Hall SCL; ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Oxfordshire, United Kingdom., Clifton LA; ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Oxfordshire, United Kingdom., Sridhar P; School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Hardy DJ; School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Wotherspoon P; School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Wright J; Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, United Kingdom., Whitehouse J; Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, United Kingdom., Gamage N; Membrane Protein Laboratory, Diamond Light Source, Harwell Science & Innovation Campus, Oxfordshire, United Kingdom., Laxton CS; School of Life Sciences, University of Nottingham, Nottingham, United Kingdom., Hatton C; School of Life Sciences, University of Warwick, Coventry, United Kingdom., Hughes GW; School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Jeeves M; Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, United Kingdom., Knowles TJ; School of Biosciences, University of Birmingham, Birmingham, United Kingdom. Electronic address: t.j.knowles@bham.ac.uk.
المصدر: Biophysical journal [Biophys J] 2021 Dec 07; Vol. 120 (23), pp. 5295-5308. Date of Electronic Publication: 2021 Oct 30.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-0086 (Electronic) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge, MA : Cell Press
Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society.
مواضيع طبية MeSH: Escherichia coli Proteins*, Bacterial Outer Membrane Proteins ; Escherichia coli ; Protein Folding
مستخلص: The outer membrane of Gram-negative bacteria presents a robust physicochemical barrier protecting the cell from both the natural environment and acting as the first line of defense against antimicrobial materials. The proteins situated within the outer membrane are responsible for a range of biological functions including controlling influx and efflux. These outer membrane proteins (OMPs) are ultimately inserted and folded within the membrane by the β-barrel assembly machine (Bam) complex. The precise mechanism by which the Bam complex folds and inserts OMPs remains unclear. Here, we have developed a platform for investigating Bam-mediated OMP insertion. By derivatizing a gold surface with a copper-chelating self-assembled monolayer, we were able to assemble a planar system containing the complete Bam complex reconstituted within a phospholipid bilayer. Structural characterization of this interfacial protein-tethered bilayer by polarized neutron reflectometry revealed distinct regions consistent with known high-resolution models of the Bam complex. Additionally, by monitoring changes of mass associated with OMP insertion by quartz crystal microbalance with dissipation monitoring, we were able to demonstrate the functionality of this system by inserting two diverse OMPs within the membrane, pertactin, and OmpT. This platform has promising application in investigating the mechanism of Bam-mediated OMP insertion, in addition to OMP function and activity within a phospholipid bilayer environment.
(Copyright © 2021 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: BB/P0098401 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/S017283/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
المشرفين على المادة: 0 (Bacterial Outer Membrane Proteins)
0 (Escherichia coli Proteins)
تواريخ الأحداث: Date Created: 20211110 Date Completed: 20211214 Latest Revision: 20240405
رمز التحديث: 20240405
مُعرف محوري في PubMed: PMC8715194
DOI: 10.1016/j.bpj.2021.10.033
PMID: 34757080
قاعدة البيانات: MEDLINE
الوصف
تدمد:1542-0086
DOI:10.1016/j.bpj.2021.10.033