دورية أكاديمية
أعرض في EDS

LuNER: Multiplexed SARS-CoV-2 detection in clinical swab and wastewater samples.

التفاصيل البيبلوغرافية
العنوان: LuNER: Multiplexed SARS-CoV-2 detection in clinical swab and wastewater samples.
المؤلفون: Stahl EC; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Gopez AR; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Tsuchida CA; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Fan VB; University of California, Berkeley, Berkeley, CA, United States of America., Moehle EA; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Witkowsky LB; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Hamilton JR; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Lin-Shiao E; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., McElroy M; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., McDevitt SL; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Ciling A; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Tsui CK; University of California, Berkeley, Berkeley, CA, United States of America., Pestal K; University of California, Berkeley, Berkeley, CA, United States of America., Gildea HK; University of California, Berkeley, Berkeley, CA, United States of America., Keller A; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Sylvain IA; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Williams C; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Hirsh A; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Ehrenberg AJ; University of California, Berkeley, Berkeley, CA, United States of America., Kantor R; University of California, Berkeley, Berkeley, CA, United States of America.; Department of Civil and Environmental Engineering, University of California, Berkeley, CA, United States of America., Metzger M; University of California, Berkeley, Berkeley, CA, United States of America.; Department of Civil and Environmental Engineering, University of California, Berkeley, CA, United States of America., Nelson KL; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America.; Department of Civil and Environmental Engineering, University of California, Berkeley, CA, United States of America., Urnov FD; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Ringeisen BR; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Giannikopoulos P; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America., Doudna JA; University of California, Berkeley, Berkeley, CA, United States of America.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, United States of America.; Howard Hughes Medical Institute, University of California, Berkeley, CA, United States of America.
مؤلفون مشاركون: IGI Testing Consortium
المصدر: PloS one [PLoS One] 2021 Nov 10; Vol. 16 (11), pp. e0258263. Date of Electronic Publication: 2021 Nov 10 (Print Publication: 2021).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: COVID-19/*virology , Ribonuclease P/*genetics , SARS-CoV-2/*genetics , Wastewater/*virology, DNA Primers/genetics ; Humans ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Specimen Handling/methods ; Wastewater-Based Epidemiological Monitoring
مستخلص: Clinical and surveillance testing for the SARS-CoV-2 virus relies overwhelmingly on RT-qPCR-based diagnostics, yet several popular assays require 2-3 separate reactions or rely on detection of a single viral target, which adds significant time, cost, and risk of false-negative results. Furthermore, multiplexed RT-qPCR tests that detect at least two SARS-CoV-2 genes in a single reaction are typically not affordable for large scale clinical surveillance or adaptable to multiple PCR machines and plate layouts. We developed a RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (LuNER) to address these shortcomings and meet the testing demands of a university campus and the local community. This cost-effective test is compatible with BioRad or Applied Biosystems qPCR machines, in 96 and 384-well formats, with or without sample pooling, and has a detection sensitivity suitable for both clinical reporting and wastewater surveillance efforts.
Competing Interests: The authors have read the journal’s policy and the authors of this manuscript have the following competing interests: The Regents of the University of California have patents issued and pending for CRISPR technologies on which J.A.D. is an inventor. J.A.D. is a cofounder of Caribou Biosciences, Editas Medicine, Scribe Therapeutics, Intellia Therapeutics and Mammoth Biosciences. J.A.D. is a scientific advisory board member of Caribou Biosciences, Intellia Therapeutics, eFFECTOR Therapeutics, Scribe Therapeutics, Mammoth Biosciences, Synthego, Algen Biotechnologies, Felix Biosciences and Inari. J.A.D. is a Director at Johnson & Johnson and has research projects sponsored by Biogen, Pfizer, AppleTree Partners and Roche. F.D.U. is a co-founder of Tune Therapeutics. P.G. is a cofounder and Director at NewCo Health. P.G. is the CLIA Laboratory Director for Coral Genomics and 3DMed. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
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معلومات مُعتمدة: 1F31 HL156468-01 United States NH NIH HHS; F31 HL156468 United States HL NHLBI NIH HHS; T32 GM132022 United States GM NIGMS NIH HHS; T32 GM139780 United States GM NIGMS NIH HHS; F32 GM142146-01 United States NH NIH HHS; F32 GM140637 United States GM NIGMS NIH HHS; F32 GM140637-01 United States NH NIH HHS; F32 GM142146 United States GM NIGMS NIH HHS; F99 AG068343 United States AG NIA NIH HHS; 1F99 AG068343-01 United States NH NIH HHS
المشرفين على المادة: 0 (DNA Primers)
0 (RNA, Viral)
0 (Waste Water)
EC 3.1.26.5 (Ribonuclease P)
تواريخ الأحداث: Date Created: 20211110 Date Completed: 20211124 Latest Revision: 20231103
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8580221
DOI: 10.1371/journal.pone.0258263
PMID: 34758033
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0258263