دورية أكاديمية
Citral modulates human monocyte responses to Staphylococcus aureus infection.
| العنوان: | Citral modulates human monocyte responses to Staphylococcus aureus infection. |
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| المؤلفون: | Oliveira HBM; Universidade Estadual de Santa Cruz, Rod. Jorge Amado, Km a6, Salobrinho, Ilhéus, Bahia, 55662-900, Brazil., das Neves Selis N; Universidade Estadual de Santa Cruz, Rod. Jorge Amado, Km a6, Salobrinho, Ilhéus, Bahia, 55662-900, Brazil., Brito TLS; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista, Bahia, 45029-094, Brazil., Sampaio BA; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista, Bahia, 45029-094, Brazil., de Souza Bittencourt R; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista, Bahia, 45029-094, Brazil., Oliveira CNT; Universidade Estadual de Santa Cruz, Rod. Jorge Amado, Km a6, Salobrinho, Ilhéus, Bahia, 55662-900, Brazil.; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista, Bahia, 45029-094, Brazil., Júnior MNS; Universidade Estadual de Santa Cruz, Rod. Jorge Amado, Km a6, Salobrinho, Ilhéus, Bahia, 55662-900, Brazil., Almeida CF; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista, Bahia, 45029-094, Brazil., Almeida PP; Departamento de Biologia Geral, Universidade Federal da Viçosa, Av. Peter Henry Rolfs s/n, Campus Universitário, Viçosa, Minas Gerais, CEP: 36570-000, Brazil., Campos GB; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista, Bahia, 45029-094, Brazil., Amorim AT; Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã, São Paulo, 05508-900, Brazil., Timenetsky J; Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã, São Paulo, 05508-900, Brazil., Romano CC; Universidade Estadual de Santa Cruz, Rod. Jorge Amado, Km a6, Salobrinho, Ilhéus, Bahia, 55662-900, Brazil., Uetanabaro APT; Universidade Estadual de Santa Cruz, Rod. Jorge Amado, Km a6, Salobrinho, Ilhéus, Bahia, 55662-900, Brazil., Yatsuda R; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista, Bahia, 45029-094, Brazil., Marques LM; Universidade Estadual de Santa Cruz, Rod. Jorge Amado, Km a6, Salobrinho, Ilhéus, Bahia, 55662-900, Brazil. lmirandamarques@gmail.com.; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista, Bahia, 45029-094, Brazil. lmirandamarques@gmail.com. |
| المصدر: | Scientific reports [Sci Rep] 2021 Nov 11; Vol. 11 (1), pp. 22029. Date of Electronic Publication: 2021 Nov 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Acyclic Monoterpenes/*pharmacology , Immunologic Factors/*pharmacology , Monocytes/*drug effects , Staphylococcal Infections/*immunology , Staphylococcus aureus/*immunology, Adult ; Brazil ; Cells, Cultured ; Child ; Humans ; Inflammation/complications ; Inflammation/drug therapy ; Inflammation/immunology ; Male ; Monocytes/immunology ; Staphylococcal Infections/complications ; Staphylococcal Infections/drug therapy ; Staphylococcus aureus/drug effects ; Young Adult |
| مستخلص: | Staphylococcus aureus is a Gram-positive bacterium that is considered an important human pathogen. Due to its virulence and ability to acquire mechanisms of resistance to antibiotics, the clinical severity of S. aureus infection is driven by inflammatory responses to the bacteria. Thus, the present study aimed to investigate the modulating role of citral in inflammation caused by S. aureus infection. For this, we used an isolate obtained from a nasal swab sample of a healthy child attending a day-care centre in Vitória da Conquista, Bahia, Brazil. The role of citral in modulating immunological factors against S. aureus infection was evaluated by isolating and cultivating human peripheral blood mononuclear cells. The monocytes were treated with 4%, 2%, and 1% citral before and after inoculation with S. aureus. The cells were analysed by immunophenotyping of monocyte cell surface molecules (CD54, CD282, CD80, HLA-DR, and CD86) and cytokine dosage (IL-1β, IL-6, IL-10, IL-12p70, IL-23, IFN-γ, TGF-β, and TNF-α), and evaluated for the expression of 84 genes related to innate and adaptive immune system responses. GraphPad Prism software and variables with P values < 0.05, were used for statistical analysis. Our data demonstrated citral's action on the expression of surface markers involved in recognition, presentation, and migration, such as CD14, CD54, and CD80, in global negative regulation of inflammation with inhibitory effects on NF-κB, JNK/p38, and IFN pathways. Consequently, IL-1β, IL-6, IL-12p70, IL-23, IFN-γ, and TNF-α cytokine expression was reduced in groups treated with citral and groups treated with citral at 4%, 2%, and 1% and infected, and levels of anti-inflammatory cytokines such as IL-10 were increased. Furthermore, citral could be used as a supporting anti-inflammatory agent against infections caused by S. aureus. There are no data correlating citral, S. aureus, and the markers analysed here; thus, our study addresses this gap in the literature. (© 2021. The Author(s).) |
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| معلومات مُعتمدة: | Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; JCB0035/2016 Fundação de Amparo em Pesquisa do Estado da Bahia |
| المشرفين على المادة: | 0 (Acyclic Monoterpenes) 0 (Immunologic Factors) T7EU0O9VPP (citral) |
| تواريخ الأحداث: | Date Created: 20211112 Date Completed: 20220131 Latest Revision: 20220131 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8586039 |
| DOI: | 10.1038/s41598-021-01536-4 |
| PMID: | 34764372 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-021-01536-4 |