دورية أكاديمية
أعرض في EDS

Steroid Metabolism in Children and Adolescents With Obesity and Insulin Resistance: Altered SRD5A and 20α/20βHSD Activity.

التفاصيل البيبلوغرافية
العنوان: Steroid Metabolism in Children and Adolescents With Obesity and Insulin Resistance: Altered SRD5A and 20α/20βHSD Activity.
المؤلفون: Sumińska M; Department of Pediatric Diabetes and Obesity, Institute of Pediatrics, Poznan University of Medical Sciences, Poznan, Poland., Podgórski R; Centre for Innovative Research in Medical and Natural Sciences, University of Rzeszow, Rzeszow, Poland.; Department of Biochemistry, Institute of Medical Sciences, Collegium of Medical Sciences, University of Rzeszow, Rzeszow, Poland., Fichna P; Department of Pediatric Diabetes and Obesity, Institute of Pediatrics, Poznan University of Medical Sciences, Poznan, Poland., Fichna M; Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland.
المصدر: Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2021 Oct 26; Vol. 12, pp. 759971. Date of Electronic Publication: 2021 Oct 26 (Print Publication: 2021).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: Insulin Resistance*, 20-alpha-Hydroxysteroid Dehydrogenase/*metabolism , 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/*metabolism , Cortisone Reductase/*metabolism , Membrane Proteins/*metabolism , Pediatric Obesity/*enzymology, Adolescent ; Case-Control Studies ; Child ; Female ; Humans ; Male ; Steroids/urine
مستخلص: Alterations in glucocorticoid metabolism may contribute to the development of obesity and insulin resistance (IR). Obesity in turn affects the androgen balance. The peripheral metabolism of steroids is equally an important determinant of their bioavailability and activity. The aim of this study was to evaluate steroid metabolism in obese children and to define which enzyme alterations are associated with IR. Clinical characteristics and anthropometric measurements were determined in 122 obese children and adolescents (72 girls, 50 boys) aged 8 - 18 years. 26 of them (21.3%) were diagnosed with IR (13 boys, 13 girls). Routine laboratory tests were performed and 24h urinary steroid excretion profiles were analyzed by gas chromatography/mass spectrometry. Positive relationship between 5α-reductase (SRD5A) activity and IR was found. According to the androsterone to etiocholanolone (An/Et) ratio the activity of SRD5A was significantly increased in obese children with IR, but the difference remained insignificant once the 5α-dihydrotestosterone to testosterone (5αDHT/T) ratio was considered. Furthermore, this relationship persisted in boys but was not observed in girls. The activity of 20α-hydroxysteroid dehydrogenase (20αHSD) and 20β-hydroxysteroid dehydrogenase (20βHSD) was reduced only in obese girls with IR. Conclude, in the context of obese children and adolescents with IR, we surmise that increased SRD5A represents a compensatory mechanism to reduce local glucocorticoid availability. This phenomenon is probably different in the liver (restriction) and in the adipose tissue (expected increase in activity). We show significant changes in 20αHSD and 20βHSD activity in obese girls with IR, but it is difficult to clearly determine whether the activity of these enzymes is an indicator of the function in their ovaries or adrenal glands.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Sumińska, Podgórski, Fichna and Fichna.)
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فهرسة مساهمة: Keywords: 20α-hydroxysteroid dehydrogenase; 20β-hydroxysteroid dehydrogenase; 5α-reductase; adolescents; children; insulin resistance; obesity; urinary steroid metabolites
المشرفين على المادة: 0 (Membrane Proteins)
0 (Steroids)
EC 1.1.1.149 (20-alpha-Hydroxysteroid Dehydrogenase)
EC 1.1.1.53 (Cortisone Reductase)
EC 1.3.99.5 (3-Oxo-5-alpha-Steroid 4-Dehydrogenase)
EC 1.3.99.5 (SRD5A1 protein, human)
تواريخ الأحداث: Date Created: 20211112 Date Completed: 20220218 Latest Revision: 20220218
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC8577858
DOI: 10.3389/fendo.2021.759971
PMID: 34764940
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-2392
DOI:10.3389/fendo.2021.759971