دورية أكاديمية
أعرض في EDS

Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications.

التفاصيل البيبلوغرافية
العنوان: Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications.
المؤلفون: Dymova MA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia., Utkin YA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia., Dmitrieva MD; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia., Kuligina EV; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia., Richter VA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2021 Oct 29; Vol. 26 (21). Date of Electronic Publication: 2021 Oct 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Bacteriophages/*chemistry , Breast Neoplasms/*diagnosis , Coloring Agents/*chemistry, Breast Neoplasms/drug therapy ; Cell Line, Tumor ; Female ; Humans
مستخلص: Background: Tumor-targeting bacteriophages can be used as a versatile new platform for the delivery of diagnostic imaging agents and therapeutic cargo. This became possible due to the development of viral capsid modification method. Earlier in our laboratory and using phage display technology, phages to malignant breast cancer cells MDA-MB 231 were obtained. The goal of this study was the optimization of phage modification and the assessment of the effect of the latter on the efficiency of phage particle penetration into MDA-MB 231 cells.
Methods: In this work, we used several methods, such as chemical phage modification using FAM-NHS ester, spectrophotometry, phage amplification, sequencing, phage titration, flow cytometry, and confocal microscopy.
Results: We performed chemical phage modification using different concentrations of FAM-NHS dye (0.5 mM, 1 mM, 2 mM, 4 mM, 8 mM). It was shown that with an increase of the modification degree, the phage titer decreases. The maximum modification coefficient of the phage envelope with the FAM-NHS dye was observed with 4 mM modifying agent and had approximately 804,2 FAM molecules per phage. Through the immunofluorescence staining and flow cytometry methods, it was shown that the modified bacteriophage retains the ability to internalize into MDA-MB-231 cells. The estimation of the number of phages that could have penetrated into one tumor cell was conducted.
Conclusions: Optimizing the conditions for phage modification can be an effective strategy for producing tumor-targeting diagnostic and therapeutic agents, i.e., theranostic drugs.
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معلومات مُعتمدة: 19-44-02006 Russian Science Foundation
فهرسة مساهمة: Keywords: FAM–NHS; MDA-MB 231 cells; chemical modification of bacteriophages; theranostic drugs; tumor-targeting bacteriophages
المشرفين على المادة: 0 (Coloring Agents)
تواريخ الأحداث: Date Created: 20211113 Date Completed: 20211203 Latest Revision: 20211214
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8588016
DOI: 10.3390/molecules26216564
PMID: 34770973
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules26216564