دورية أكاديمية
Identification of plasmids from Brazilian Chromobacterium violaceum strains.
| العنوان: | Identification of plasmids from Brazilian Chromobacterium violaceum strains. |
|---|---|
| المؤلفون: | de Lima DC; Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte, CEP 59015-000, Natal, Brazil.; Laboratório de Biologia Molecular e Genômica, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil., Medeiros IG; Laboratório de Biologia Molecular e Genômica, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil.; Programa de Pós-Graduação em Bioinformática, Instituto Metrópole Digital, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil.; Instituto do Cérebro, Universidade Federal do Rio Grande do Norte, CEP 59056-450, Natal, Brazil., de Cássia Silva-Portela R; Laboratório de Biologia Molecular e Genômica, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil., da Silva Junior FC; Laboratório de Biologia Molecular e Genômica, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil., Fassarela Agnez-Lima L; Laboratório de Biologia Molecular e Genômica, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil.; Programa de Pós-Graduação em Bioinformática, Instituto Metrópole Digital, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil., de Souza JES; Bioinformatics Multidisciplinary Environment, Instituto Metrópole Digital, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil.; Programa de Pós-Graduação em Bioinformática, Instituto Metrópole Digital, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil., Batistuzzo de Medeiros SR; Laboratório de Biologia Molecular e Genômica, Universidade Federal do Rio Grande do Norte, CEP 59078-970, Natal, Brazil. |
| المصدر: | Canadian journal of microbiology [Can J Microbiol] 2021 Nov 15, pp. 1-10. Date of Electronic Publication: 2021 Nov 15. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 0372707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1480-3275 (Electronic) Linking ISSN: 00084166 NLM ISO Abbreviation: Can J Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011- : Ottawa, ON : Canadian Science Publishing Original Publication: Ottawa, Canada : National Research Council, [1954- |
| مستخلص: | Chromobacterium violaceum is an opportunistic pathogen found in tropical and subtropical regions worldwide. Chromobacterium violaceum infections are difficult to treat, and many strains are resistant to antibiotics. Recently, a novel plasmid (pChV1) was discovered in the type strain ATCC 12472, suggesting that other C. violaceum strains may harbor extra-chromosomal DNA. The aim of the present study was to detect and compare new plasmids in Brazilian strains of C. violaceum using next-generation sequencing techniques. We obtained draft genomes of six plasmids from strains isolated from the Amazon region and aligned them with pChV1. At least three plasmids, CVAC05, CVACO2, and CVT8, were similar to pChV1. Phylogenetic analysis suggested that these new extra-chromosomal DNA sequences have a common origin with pChV1 but have diverged. Many of the ORFs detected were related to plasmid segregation/maintenance, viral structural proteins, and proteins with unknown functions. These findings may enable better genetic manipulation of C. violaceum , which will enhance our ability to exploit this valuable microorganism in industrial and clinical applications. |
| فهرسة مساهمة: | Keywords: antibiotic resistance; bacteriophages; bactériophages; biotechnologie; biotechnology; genomics; génomique; microbial phylogenetics; phylogénétique microbienne; plasmides; plasmids; résistance aux antibiotiques |
| تواريخ الأحداث: | Date Created: 20211115 Latest Revision: 20240220 |
| رمز التحديث: | 20240220 |
| DOI: | 10.1139/cjm-2021-0197 |
| PMID: | 34780296 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1480-3275 |
|---|---|
| DOI: | 10.1139/cjm-2021-0197 |