دورية أكاديمية
أعرض في EDS

SARS-CoV-2 monitoring at three sewersheds of different scales and complexity demonstrates distinctive relationships between wastewater measurements and COVID-19 case data.

التفاصيل البيبلوغرافية
العنوان: SARS-CoV-2 monitoring at three sewersheds of different scales and complexity demonstrates distinctive relationships between wastewater measurements and COVID-19 case data.
المؤلفون: Nagarkar M; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Nagarkar.Maitreyi@epa.gov., Keely SP; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Keely.Scott@epa.gov., Jahne M; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Jahne.Michael@epa.gov., Wheaton E; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Wheaton.Emily@epa.gov., Hart C; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Hart.Chloe@epa.gov., Smith B; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Smith.Bruce@epa.gov., Garland J; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Garland.Jay@epa.gov., Varughese EA; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Varughese.Eunice@epa.gov., Braam A; APTIM Corp., 4171 Essen Lane, Baton Rouge, LA 70809. Electronic address: Braam.Ana@epa.gov., Wiechman B; APTIM Corp., 4171 Essen Lane, Baton Rouge, LA 70809. Electronic address: Wiechman.Barry@epa.gov., Morris B; Pegasus Technical Services Inc., 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Morris.Brian@epa.gov., Brinkman NE; Office of Research and Development, United States Environmental Protection Agency, 26 W Martin Luther King Dr, Cincinnati, OH, USA. Electronic address: Brinkman.Nichole@epa.gov.
المصدر: The Science of the total environment [Sci Total Environ] 2022 Apr 10; Vol. 816, pp. 151534. Date of Electronic Publication: 2021 Nov 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier.
مواضيع طبية MeSH: COVID-19* , SARS-CoV-2*, Humans ; Prevalence ; RNA ; Wastewater
مستخلص: Wastewater monitoring of SARS-CoV-2 presents a means of tracking COVID-19 community infection dynamics on a broader geographic scale. However, accounting for environmental and sample-processing losses may be necessary for wastewater measurements to readily inform our understanding of infection prevalence. Here, we present measurements of the SARS-CoV-2 N1 and N2 gene targets from weekly wastewater samples at three sites in Hamilton County, Ohio, during an increase and subsequent decline of COVID-19 infections. The concentration of N1 or N2 RNA in wastewater, measured over the course of six months, ranged from below the detection limit to over 10 4 gene copies/l, and correlated with case data at two wastewater treatment plants, but not at a sub-sewershed-level sampling site. We also evaluated the utility of a broader range of variables than has been reported consistently in previous work, in improving correlations of SARS-CoV-2 concentrations with case data. These include a spiked matrix recovery control (OC43), flow-normalization, and assessment of fecal loading using endogenous fecal markers (HF183, PMMoV, crAssphage). We found that adjusting for recovery, flow, and fecal indicators increased these correlations for samples from a larger sewershed (serving ~488,000 people) with greater industrial and stormwater inputs, but raw N1/N2 concentrations corresponded better with case data at a smaller, residential-oriented sewershed. Our results indicate that the optimal adjustment factors for correlating wastewater and clinical case data moving forward may not be generalizable to all sewersheds.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier B.V.)
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فهرسة مساهمة: Keywords: Environmental surveillance; Fecal indicators; SARS-CoV-2; Wastewater; ddPCR
المشرفين على المادة: 0 (Waste Water)
63231-63-0 (RNA)
تواريخ الأحداث: Date Created: 20211115 Date Completed: 20220216 Latest Revision: 20221217
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8590472
DOI: 10.1016/j.scitotenv.2021.151534
PMID: 34780821
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1026
DOI:10.1016/j.scitotenv.2021.151534