دورية أكاديمية
أعرض في EDS

SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument.

التفاصيل البيبلوغرافية
العنوان: SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument.
المؤلفون: Rosenstierne MW; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark. Mwr@egoo.health.; Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark. Mwr@egoo.health., Joshi S; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Danielsen ET; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Webb H; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Luong DM; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Bjerring J; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Hindkær J; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Jørgensen L; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Blauenfeldt J; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Bojesen A; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Holck F; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Lau JW; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Bangsgaard L; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Lind JB; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Dragheim MB; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Jacobsen MR; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Elkjær R; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Clauwaert S; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Christensen K; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Polacek C; Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark., Fomsgaard A; Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark., Ojalehto T; Aidian Oy, Espoo, Finland., Tullila A; Aidian Oy, Espoo, Finland., Brummer M; Aidian Oy, Espoo, Finland., Jensen CJ; Klinisk Biokemisk Afdeling, Nordsjællands Hospital, Dyrehavevej 29, 3400, Hillerød, Denmark., Jensen FH; Amager og Hvidovre Hospital, Klinisk Mikrobiologisk Afdeling, afsnit 445, Kettegård Allé 30, 2650, Hvidovre, Denmark., Schneider UV; Amager og Hvidovre Hospital, Klinisk Mikrobiologisk Afdeling, afsnit 445, Kettegård Allé 30, 2650, Hvidovre, Denmark., Lisby JG; Amager og Hvidovre Hospital, Klinisk Mikrobiologisk Afdeling, afsnit 445, Kettegård Allé 30, 2650, Hvidovre, Denmark., Jørgensen RL; Amager og Hvidovre Hospital, Klinisk Mikrobiologisk Afdeling, afsnit 445, Kettegård Allé 30, 2650, Hvidovre, Denmark., Warthoe T; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Finding E; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark., Warthoe P; Qlife, Borupvang 3, 2750 Ballerup & Symbion, Fruebjergvej 3, 2100, Copenhagen, Denmark.
المصدر: Scientific reports [Sci Rep] 2021 Nov 15; Vol. 11 (1), pp. 22214. Date of Electronic Publication: 2021 Nov 15.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Equipment Design*, COVID-19/*diagnosis , COVID-19/*epidemiology , Molecular Diagnostic Techniques/*instrumentation , Molecular Diagnostic Techniques/*methods , Pandemics/*prevention & control , Reverse Transcriptase Polymerase Chain Reaction/*instrumentation , Reverse Transcriptase Polymerase Chain Reaction/*methods , SARS-CoV-2/*genetics, COVID-19/virology ; Cell Phone ; Humans ; Mobile Applications ; Oropharynx/virology ; Point-of-Care Testing ; Polymorphism, Single Nucleotide ; RNA, Viral/genetics ; Retrospective Studies ; Sensitivity and Specificity
مستخلص: Rapid nucleic-acid based tests that can be performed by non-professionals outside laboratory settings could help the containment of the pandemic SARS-CoV-2 virus and may potentially prevent further widespread lockdowns. Here, we present a novel compact portable detection instrument (the Egoo Health System) for extraction-free detection of SARS-CoV-2 using isothermal reverse transcription strand invasion based amplification (RT-SIBA). The SARS-CoV-2 RT-SIBA assay can be performed directly on crude oropharyngeal swabs without nucleic acid extraction with a reaction time of 30 min. The Egoo Health system uses a capsule system, which is automatically sealed tight in the Egoo instrument after applying the sample, resulting in a closed system optimal for molecular isothermal amplification. The performance of the Egoo Health System is comparable to the PCR instrument with an analytical sensitivity of 25 viral RNA copies per SARS-CoV-2 RT-SIBA reaction and a clinical sensitivity and specificity between 87.0-98.4% and 96.6-98.2% respectively.
(© 2021. The Author(s).)
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المشرفين على المادة: 0 (RNA, Viral)
تواريخ الأحداث: Date Created: 20211116 Date Completed: 20211203 Latest Revision: 20211214
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8593107
DOI: 10.1038/s41598-021-01744-y
PMID: 34782681
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-021-01744-y