التفاصيل البيبلوغرافية
| العنوان: |
Derivation of Ringed Seal ( Phoca hispida ) Induced Multipotent Stem Cells. |
| المؤلفون: |
Beklemisheva VR; Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.; The preprint version of the article is available online at bioRxiv preprint server: https://www.biorxiv.org/content/10.1101/2020.05.20.105890v3., Belokopytova PS; Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.; The preprint version of the article is available online at bioRxiv preprint server: https://www.biorxiv.org/content/10.1101/2020.05.20.105890v3., Fishman VS; Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.; Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia.; The preprint version of the article is available online at bioRxiv preprint server: https://www.biorxiv.org/content/10.1101/2020.05.20.105890v3., Menzorov AG; Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.; Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia.; The preprint version of the article is available online at bioRxiv preprint server: https://www.biorxiv.org/content/10.1101/2020.05.20.105890v3. |
| المصدر: |
Cellular reprogramming [Cell Reprogram] 2021 Dec; Vol. 23 (6), pp. 326-335. Date of Electronic Publication: 2021 Nov 16. |
| نوع المنشور: |
Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101528176 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2152-4998 (Electronic) Linking ISSN: 21524971 NLM ISO Abbreviation: Cell Reprogram Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: New Rochelle, NY : Mary Ann Liebert, Inc. |
| مواضيع طبية MeSH: |
Induced Pluripotent Stem Cells* , Phoca*, Animals ; Cell Differentiation ; Cell Line ; Dogs ; Ferrets ; Multipotent Stem Cells |
| مستخلص: |
Induced pluripotent stem (iPS) cells have been produced just for a few species among order Carnivora: snow leopard, Bengal tiger, serval, jaguar, cat, dog, ferret, and American mink. We applied the iPS cell derivation protocol to the ringed seal ( Phoca hispida ) fibroblasts. The resulting cell line had the expression of pluripotency marker gene Rex1 . Differentiation in embryoid body-like structures allowed us to register expression of AFP , endoderm marker, and Cdx2 , trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably is not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipocytes and osteocytes. |
| فهرسة مساهمة: |
Keywords: Carnivora; Rex1; adipocyte differentiation; iPS cells; pluripotency; reprogramming |
| تواريخ الأحداث: |
Date Created: 20211117 Date Completed: 20220211 Latest Revision: 20220211 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1089/cell.2021.0037 |
| PMID: |
34788122 |
| قاعدة البيانات: |
MEDLINE |
