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Ovarian tissue features assessed in bovine fetuses after vitrification and xenotransplantation procedures.

التفاصيل البيبلوغرافية
العنوان: Ovarian tissue features assessed in bovine fetuses after vitrification and xenotransplantation procedures.
المؤلفون: Moraes GF; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil. Electronic address: g.f.moraes@hotmail.com., Ronda JB; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil., Campos BS; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil., Rocha CD; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil., Soares MM; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil., Gowen BH; Conception Biology, Berkeley, 94710, CA, USA., de Freitas RAA; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil., Silva Junior ED; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil., Marques JCDS; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil., Gontijo DA; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil., de Moraes ABA; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil., Brito RM; Cytogenetic Laboratory, Genetic and Biochemistry Institute, Federal University of Uberlândia, Uberlândia, MG, Brazil., de Oliveira Júnior RJ; Cytogenetic Laboratory, Genetic and Biochemistry Institute, Federal University of Uberlândia, Uberlândia, MG, Brazil., Alves BG; Conception Biology, Berkeley, 94710, CA, USA., Alves KA; Conception Biology, Berkeley, 94710, CA, USA., Santos RMD; Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil.
المصدر: Reproductive biology [Reprod Biol] 2021 Dec; Vol. 21 (4), pp. 100575. Date of Electronic Publication: 2021 Nov 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Urban & Partner Country of Publication: Poland NLM ID: 101160559 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2300-732X (Electronic) Linking ISSN: 1642431X NLM ISO Abbreviation: Reprod Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: <2013- > : Olsztyn, Poland : Elsevier Urban & Partner
Original Publication: Olsztyn, Poland : Polish Society for Biology of Reproduction, c2001-
مواضيع طبية MeSH: Cryopreservation/*veterinary , Ovary/*physiology , Tissue Preservation/*veterinary , Transplantation, Heterologous/*veterinary, Animals ; Cattle ; Female ; Fetus ; Mice ; Mice, Inbred BALB C ; Pregnancy ; Tissue Preservation/methods ; Vitrification
مستخلص: Cryopreservation and transplantation of ovarian tissue are proposed methods for the restoration of endocrine function and reproductive potential. Therefore, this study aimed to evaluate the effects of vitrification and xenotransplantation on follicle viability, activation, stromal cell integrity, vascularization, and micronuclei formation. Bovine fetal ovaries were fragmented and assigned to the following groups: Fresh control (FC), ovarian fragments immediately fixed; Vitrified control (VC), ovarian fragments vitrified; Vitrified xenotransplanted (VX), ovarian fragments vitrified and xenotransplanted; and Fresh xenotransplanted (FX), ovarian fragments xenotransplanted. Ovarian fragments were grafted in female BALB/c mice and recovered after 14 days. Follicular viability was preserved (P >  0.05) in VC group. The rate of developing follicles was greater (P <  0.05) in the FX group compared to other groups. Follicular density was higher (P <  0.05) in the VC group than the FC, VX, and FX groups. A decrease (P <  0.05) of stromal cell density was recorded after vitrification (VC vs. FX). Blood vessel density decreased in VC, VX, and FX groups compared with the FC group, and blood vessel density was correlated with follicular viability (positively; P =  0.07) and developing follicles (negatively; P <  0.001). Both vitrification and xenotransplantation groups (VC, VX, and FX) had a greater (P <  0.05) number of cells with one MN compared to the FC group. In summary, our findings showed that both vitrification and xenotransplantation modified blood vessel, follicular and stromal cell densities, follicular viability and activation, and micronuclei formation in ovarian tissue.
(Copyright © 2021 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Chromosomal abnormalities; Preantral follicles; Stromal cells; Vascularization; Vitrification
تواريخ الأحداث: Date Created: 20211122 Date Completed: 20220218 Latest Revision: 20220218
رمز التحديث: 20221213
DOI: 10.1016/j.repbio.2021.100575
PMID: 34808453
قاعدة البيانات: MEDLINE
الوصف
تدمد:2300-732X
DOI:10.1016/j.repbio.2021.100575