دورية أكاديمية
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma.
| العنوان: | Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma. |
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| المؤلفون: | Munari FF; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil., Sichero L; Center for Translational Research in Oncology, Instituto do Cancer do Estado de Sao Paulo-ICESP, Cerqueira César, Brazil., Carloni AC; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil., Lacerda CF; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil.; Department of Digestive Surgery, Barretos Cancer Hospital, Barretos, Brazil., Nunes EM; Center for Translational Research in Oncology, Instituto do Cancer do Estado de Sao Paulo-ICESP, Cerqueira César, Brazil., de Oliveira ATT; Department of Digestive Surgery, Barretos Cancer Hospital, Barretos, Brazil., Scapulatempo-Neto C; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil.; Department of Pathology, Diagnosis of Biopsies and Surgical Specimens, Barretos Cancer Hospital, Barretos, Brazil., da Silva SRM; Department of Pathology, Diagnosis of Biopsies and Surgical Specimens, Barretos Cancer Hospital, Barretos, Brazil., Crema E; Department of Digestive Surgery and Pathology, Medical School, UFTM, Federal University of Triangulo Mineiro, Uberaba, Brazil., Adad SJ; Departament of Gastroenterology Surgery and Pathology, Medical School, UNESP, São Paulo State University, Botucatu, Brazil., Rodrigues MAM; Departament of Gastroenterology Surgery and Pathology, Medical School, UNESP, São Paulo State University, Botucatu, Brazil., Henry MACA; Departament of Gastroenterology Surgery and Pathology, Medical School, UNESP, São Paulo State University, Botucatu, Brazil., Guimarães DP; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil.; Department of Endoscopy, Barretos Cancer Hospital, Barretos, Brazil., Reis RM; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil.; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal., Villa LL; Center for Translational Research in Oncology, Instituto do Cancer do Estado de Sao Paulo-ICESP, Cerqueira César, Brazil.; Department of Radiology and Oncology, Faculdade de Medicina, Universidade de Sao Paulo, São Paulo, Brazil., Longatto-Filho A; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil.; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.; Department of Radiology and Oncology, Medical School, University of São Paulo, Butanta, Brazil.; Medical Laboratory of Medical Investigation (LIM) 14, Department of Pathology, Medical School, University of São Paulo, Butanta, Brazil. |
| المصدر: | Pathobiology : journal of immunopathology, molecular and cellular biology [Pathobiology] 2022; Vol. 89 (1), pp. 29-37. Date of Electronic Publication: 2021 Oct 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: S. Karger Country of Publication: Switzerland NLM ID: 9007504 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1423-0291 (Electronic) Linking ISSN: 10152008 NLM ISO Abbreviation: Pathobiology Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel ; New York : S. Karger, c1990- |
| مواضيع طبية MeSH: | Alphapapillomavirus* , Carcinoma, Squamous Cell*/diagnosis , Carcinoma, Squamous Cell*/epidemiology , Esophageal Achalasia*/diagnosis , Esophageal Achalasia*/epidemiology , Esophageal Neoplasms*/diagnosis , Esophageal Neoplasms*/epidemiology , Esophageal Squamous Cell Carcinoma* , Papillomavirus Infections*/complications , Papillomavirus Infections*/diagnosis , Papillomavirus Infections*/epidemiology, Brazil ; DNA, Viral/genetics ; Humans ; Papillomaviridae/genetics ; Retrospective Studies |
| مستخلص: | Background: Chagasic megaesophagus (CM) as well as the presence of human papillomavirus (HPV) has been reported as etiological factors for esophageal squamous cell carcinoma (ESCC). Objective: We assessed the prevalence of HPV DNA in a series of ESCCs associated or not with CM. Data obtained were further correlated to the pathological and clinical data of affected individuals. Methods: A retrospective study was performed on 92 formalin-fixed and paraffin-embedded tissues collected from patients referred to 3 different hospitals in São Paulo, Brazil: Barretos Cancer Hospital, Barretos, São Paulo; Federal University of Triângulo Mineiro, Uberaba, Minas Gerais; and São Paulo State University, Botucatu, São Paulo. Cases were divided into 3 groups: (i) 24 patients with CM associated with ESCC (CM/ESCC); (ii) 37 patients with ESCC without CM (ESCC); and (iii) 31 patients with CM without ESCC (CM). Detection of HPV DNA was assessed in all samples by a genotyping assay combining multiplex polymerase chain reaction and bead-based Luminex technology. Results: We identified a high prevalence of high-risk HPV in patients in the CM group (12/31, 38.8%) and CM/ESCC (8/24, 33.3%), compared to individuals in the ESCC group (6/37, 16.3%). The individuals in the groups with cancer (ESCC and CM/ESCC) had a higher frequency of HPV-16 (4/9, 44.5% and 2/8, 25.0%). The other types of high-risk HPVs detected were HPV-31, 45, 51, 53, 56, 66, and 73. We also observed in some samples HPV coinfection by more than one viral type. Despite the high incidence of HPV, it did not show any association with the patient's clinical-pathological and molecular (TP53 mutation status) characteristics. Conclusion: This is the first report of the presence of HPV DNA in CM associated with ESCC. HPV infection was more presence in megaesophagus lesions. Further studies are needed to confirm and better understand the role of persistent HPV infection in patients with CM. (© 2021 S. Karger AG, Basel.) |
| فهرسة مساهمة: | Keywords: Brazil; Chagas disease; Chagasic megaesophagus; Esophageal cancer; Esophageal squamous cell carcinoma; Human papillomavirus |
| المشرفين على المادة: | 0 (DNA, Viral) |
| تواريخ الأحداث: | Date Created: 20211124 Date Completed: 20220120 Latest Revision: 20220120 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1159/000518697 |
| PMID: | 34818254 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1423-0291 |
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| DOI: | 10.1159/000518697 |