دورية أكاديمية
أعرض في EDS

Human Proximal Tubule Epithelial Cells (HK-2) as a Sensitive In Vitro System for Ochratoxin A Induced Oxidative Stress.

التفاصيل البيبلوغرافية
العنوان: Human Proximal Tubule Epithelial Cells (HK-2) as a Sensitive In Vitro System for Ochratoxin A Induced Oxidative Stress.
المؤلفون: García-Pérez E; School of Food Science, Washington State University, P.O. Box 646376, Pullman, Washington, DC 99164-6376, USA., Ryu D; Department of Animal, Veterinary, and Food Sciences, University of Idaho, 875 Perimeter Drive MS 2330, Moscow, ID 83844-2330, USA., Kim HY; Department of Biochemistry and Molecular Biology, Yeungnam University College of Medicine, Daegu 42415, Korea., Kim HD; Department of Animal, Veterinary, and Food Sciences, University of Idaho, 875 Perimeter Drive MS 2330, Moscow, ID 83844-2330, USA., Lee HJ; Department of Animal, Veterinary, and Food Sciences, University of Idaho, 875 Perimeter Drive MS 2330, Moscow, ID 83844-2330, USA.
المصدر: Toxins [Toxins (Basel)] 2021 Nov 06; Vol. 13 (11). Date of Electronic Publication: 2021 Nov 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Oxidative Stress*, Kidney Tubules, Proximal/*physiopathology , Mycotoxins/*adverse effects , Ochratoxins/*adverse effects, Animals ; Cell Line ; Humans ; Kidney Tubules, Proximal/metabolism ; LLC-PK1 Cells ; Sus scrofa ; Swine
مستخلص: Ochratoxin A (OTA) is a mycotoxin that is potentially carcinogenic to humans. Although its mechanism remains unclear, oxidative stress has been recognized as a plausible cause for the potent renal carcinogenicity observed in experimental animals. The effect of OTA on oxidative stress parameters in two cell lines of LLC-PK1 and HK-2 derived from the kidneys of pig and human, respectively, were investigated and compared. We found that the cytotoxicity of OTA on LLC-PK1 and HK-2 cells was dose- and time-dependent in both cell lines. Furthermore, increased intracellular reactive oxygen species (ROS) induced by OTA in both cell lines were observed in a time-dependent manner. Glutathione (GSH) was depleted by OTA at >48 h in HK-2 but not in LLC-PK1 cells. While the mRNA levels of glucose-6-phosphate dehydrogenase (G6PD) and glutathione peroxidase 1 (GPX1) in LLC-PK1 were down-regulated by 0.67- and 0.66-fold, respectively, those of catalase (CAT), glutathione reductase (GSR), and superoxide dismutase 1 (SOD) in HK-2 were up-regulated by 2.20-, 2.24-, and 2.75-fold, respectively, after 72 h exposure to OTA. Based on these results, we conclude that HK-2 cells are more sensitive to OTA-mediated toxicity than LLC-PK1, and OTA can cause a significant oxidative stress in HK-2 as indicated by changes in the parameter evaluated.
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فهرسة مساهمة: Keywords: HK-2; LLC-PK1; kidney cell lines; ochratoxin A (OTA); oxidative stress; renal carcinogen
المشرفين على المادة: 0 (Mycotoxins)
0 (Ochratoxins)
1779SX6LUY (ochratoxin A)
تواريخ الأحداث: Date Created: 20211125 Date Completed: 20220224 Latest Revision: 20220224
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8618226
DOI: 10.3390/toxins13110787
PMID: 34822571
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6651
DOI:10.3390/toxins13110787