دورية أكاديمية
أعرض في EDS

Observation of Bothrops atrox Snake Envenoming Blister Formation from Five Patients: Pathophysiological Insights.

التفاصيل البيبلوغرافية
العنوان: Observation of Bothrops atrox Snake Envenoming Blister Formation from Five Patients: Pathophysiological Insights.
المؤلفون: Gimenes SNC; Laboratório de Imunopatologia, Instituto Butantan, São Paulo 05503-900, SP, Brazil., Sachett JAG; Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus 69050-030, AM, Brazil.; Departamento de Ensino e Pesquisa, Fundação de Dermatologia Alfredo da Matta, Manaus 69065-130, AM, Brazil., Colombini M; Laboratório de Imunopatologia, Instituto Butantan, São Paulo 05503-900, SP, Brazil., Freitas-de-Sousa LA; Laboratório de Imunopatologia, Instituto Butantan, São Paulo 05503-900, SP, Brazil., Ibiapina HNS; Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus 69050-030, AM, Brazil.; Departamento de Ensino e Pesquisa, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus 69040-000, AM, Brazil., Costa AG; Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus 69050-030, AM, Brazil.; Departamento de Ensino e Pesquisa, Fundação de Hematologia e Hemoterapia do Amazonas, Manaus 69040-010, AM, Brazil., Santana MF; Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus 69050-030, AM, Brazil.; Departamento de Ensino e Pesquisa, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus 69040-000, AM, Brazil.; Departamento de Ensino e Pesquisa, Fundação de Hematologia e Hemoterapia do Amazonas, Manaus 69040-010, AM, Brazil., Park JJ; School of Medicine, University of Virginia, Charlottesville, VA 22903, USA., Sherman NE; School of Medicine, University of Virginia, Charlottesville, VA 22903, USA., Ferreira LCL; Departamento de Ensino e Pesquisa, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus 69040-000, AM, Brazil., Wen FH; Núcleo de Produção de Soros, Instituto Butantan, São Paulo 05503-900, SP, Brazil., Monteiro WM; Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus 69050-030, AM, Brazil.; Departamento de Ensino e Pesquisa, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus 69040-000, AM, Brazil., Moura-da-Silva AM; Laboratório de Imunopatologia, Instituto Butantan, São Paulo 05503-900, SP, Brazil.; Departamento de Ensino e Pesquisa, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus 69040-000, AM, Brazil., Fox JW; School of Medicine, University of Virginia, Charlottesville, VA 22903, USA.
المصدر: Toxins [Toxins (Basel)] 2021 Nov 13; Vol. 13 (11). Date of Electronic Publication: 2021 Nov 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Antivenins/*administration & dosage , Blister/*metabolism , Crotalid Venoms/*toxicity , Snake Bites/*complications, Animals ; Antivenins/metabolism ; Bothrops ; Brazil ; Crotalid Venoms/antagonists & inhibitors ; Female ; Humans ; Male ; Proteomics ; Snake Bites/therapy
مستخلص: In the Brazilian Amazon, Bothrops atrox snakebites are frequent, and patients develop tissue damage with blisters sometimes observed in the proximity of the wound. Antivenoms do not seem to impact blister formation, raising questions regarding the mechanisms underlying blister formation. Here, we launched a clinical and laboratory-based study including five patients who followed and were treated by the standard clinical protocols. Blister fluids were collected for proteomic analyses and molecular assessment of the presence of venom and antivenom. Although this was a small patient sample, there appeared to be a correlation between the time of blister appearance (shorter) and the amount of venom present in the serum (higher). Of particular interest was the biochemical identification of both venom and antivenom in all blister fluids. From the proteomic analysis of the blister fluids, all were observed to be a rich source of damage-associated molecular patterns (DAMPs), immunomodulators, and matrix metalloproteinase-9 (MMP-9), suggesting that the mechanisms by which blisters are formed includes the toxins very early in envenomation and continue even after antivenom treatment, due to the pro-inflammatory molecules generated by the toxins in the first moments after envenomings, indicating the need for local treatments with anti-inflammatory drugs plus toxin inhibitors to prevent the severity of the wounds.
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فهرسة مساهمة: Keywords: Bothrops atrox; DAMPs; antivenom; blister; local damage; snake venom; snakebite
المشرفين على المادة: 0 (Antivenins)
0 (Crotalid Venoms)
تواريخ الأحداث: Date Created: 20211125 Date Completed: 20220222 Latest Revision: 20220222
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8618272
DOI: 10.3390/toxins13110800
PMID: 34822585
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6651
DOI:10.3390/toxins13110800