دورية أكاديمية
EVALUATION THE EFFECT OF PRODIGIOSIN ON ALGD EXPRESSION IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA.
| العنوان: | EVALUATION THE EFFECT OF PRODIGIOSIN ON ALGD EXPRESSION IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA. |
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| المؤلفون: | Dhaif SS; AL-TURATH UNIVERSITY COLLEGE, BAGHDAD, IRAQ., Al-Attar NS; UNIVERSITY OF BAGHDAD, BAGHDAD, IRAQ. |
| المصدر: | Wiadomosci lekarskie (Warsaw, Poland : 1960) [Wiad Lek] 2021; Vol. 74 (9 cz 2), pp. 2265-2276. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Aluna Publishing Country of Publication: Poland NLM ID: 9705467 Publication Model: Print Cited Medium: Print ISSN: 0043-5147 (Print) Linking ISSN: 00435147 NLM ISO Abbreviation: Wiad Lek Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Konstancin Jeziorna : Aluna Publishing Original Publication: Warszawa : Pánstwowy Zakład Wydawnictw Lekarskich, [1960- |
| مواضيع طبية MeSH: | Prodigiosin*/pharmacology , Pseudomonas aeruginosa*/genetics, Anti-Bacterial Agents/pharmacology ; Ciprofloxacin ; Humans ; Microbial Sensitivity Tests |
| مستخلص: | Objective: The aim of this study is to investigate the role of prodigiosin on P. aeruginosa' s biofilm genes involved in the pathogenicity and persistency of the bacteria. Patients and Methods: Materials and methods: Gram negative bacterial isolates were taken from burn and wounds specimen obtained from some of Baghdad hospitals. Forty six isolates were identified as Pseudomonas aeruginosa and four isolates as Serratia marcescens by using biochemical tests and VITEK 2 compact system. Susceptibility test was performed for all P. aeruginosa isolates, the results showed that 100% were resistant to Amikacin and 98% were sensitive to Meropenem. Resistant isolates were tested for biofilm formation; the strong and moderate isolates (17) were detected by PCR for AlgD gene presence. From 17 isolates only two had AlgD gene. All serratia isolates were screened for prodigiosin production, which were extracted from the best producer isolate. Minimal inhibitory concentration was assessed for prodigiosin and ciprofloxacin and synergism between them against the two isolates of P. aeruginosa. Results: Results and conclusions: The results showed that the synergistic effect decreased MIC of both prodigiosin and ciprofloxacin by combination, and reduction of biofilm formation was detected. RNA was extracted from the two selected isolates as control in addition to three treatments. The result of quantitative real time PCR showed down regulation in the AlgD gene expression level under some treatments, while there was no gene expression in most treatments with both sub-MICs treatment. |
| فهرسة مساهمة: | Keywords: 16srRNA; AlgD gene; Pseudomonas aeruginosa; ciprofloxacin; prodigiosin |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 5E8K9I0O4U (Ciprofloxacin) OL369FU7CJ (Prodigiosin) |
| تواريخ الأحداث: | Date Created: 20211126 Date Completed: 20211129 Latest Revision: 20211129 |
| رمز التحديث: | 20231215 |
| PMID: | 34824170 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0043-5147 |
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