دورية أكاديمية
أعرض في EDS

Simultaneous measurement of 18 steroids in human and mouse serum by liquid chromatography-mass spectrometry without derivatization to profile the classical and alternate pathways of androgen synthesis and metabolism.

التفاصيل البيبلوغرافية
العنوان: Simultaneous measurement of 18 steroids in human and mouse serum by liquid chromatography-mass spectrometry without derivatization to profile the classical and alternate pathways of androgen synthesis and metabolism.
المؤلفون: Desai R; ANZAC Research Institute, University of Sydney, Sydney, NSW 2139, Australia., Harwood DT; Cawthron Institute, Nelson, New Zealand., Handelsman DJ; ANZAC Research Institute, University of Sydney, Sydney, NSW 2139, Australia.
المصدر: Clinical mass spectrometry (Del Mar, Calif.) [Clin Mass Spectrom] 2019 Jan 06; Vol. 11, pp. 42-51. Date of Electronic Publication: 2019 Jan 06 (Print Publication: 2019).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101697500 Publication Model: eCollection Cited Medium: Internet ISSN: 2376-9998 (Electronic) Linking ISSN: 22138005 NLM ISO Abbreviation: Clin Mass Spectrom Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier, [2016]-[2020]
مستخلص: Background: The recently identified alternate, or backdoor, pathway of DHT synthesis provides important novel information on androgen biosynthesis beyond the classical pathway. We report a rapid and versatile liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to simultaneously and accurately quantify key steroids in human or mouse serum involved in either the classical or backdoor androgen synthesis pathways.
Methods: Serum (200 µL) fortified with isotopically labelled internal standards underwent liquid-liquid extraction (LLE) with MTBE and extracts were analysed on a LC-MS/MS. The targeted steroids for quantification were testosterone (T), dihydrotestosterone (DHT), 5α-androstane-3α,17β-diol (3α diol), 5α-androstane-3β,17β-diol (3β diol), dehydroepiandrosterone (DHEA), androstenedione (A4), androsterone (AD), estradiol (E2), estrone (E1), progesterone (P4), pregnenolone (P5), androstenediol (Adiol), 17-hydroxyprogesterone (17-OHP4) and 17-hydroxypregnenolone (17-OHP5), corticosterone (B), cortisol (F), allopregnanolone (Allo-P5) and dihydroprogesterone (DHP).
Results: The limits of quantification (LOQ) were 5 pg/mL for E2 and E1, 25 pg/mL for T, 50 pg/mL for A4 and 0.10 ng/mL for DHT, 17OHP5, P4, P5, AD, Adiol, DHEA, AlloP5 and 0.20 ng/mL for 17OHP4, 3α diol, 3β diol, DHP, 0.25 ng/mL for B and 1 ng/mL for F. Accuracy, precision, reproducibility and recovery were within acceptable limits for bioanalytical method validation. The method is illustrated in human and mouse, male and female serum.
Conclusions: The presented method is sufficiently sensitive, specific and reproducible to meet the quality criteria for routine laboratory application for accurate quantitation of 18 steroid concentrations in male and female serum from humans or mice for the purpose of profiling androgen synthesis and metabolism pathways.
(Crown Copyright © 2019 Published by Elsevier B.V. on behalf of The Association for Mass Spectrometry: Applications to the Clinical Lab (MSACL).)
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فهرسة مساهمة: Keywords: 17OHP4, 17-hydroxyprogesterone; 17OHP5, 17hydroxypregnenolone; 3α diol, 5α-androstane-3α17β-diol; 3β diol, 5α-androstane-3β17β-diol; A4, androstenedione; AD, androsterone; APPI, atmospheric pressure photoionization; Adiol, androstenediol; AlloP5, allopregnanolone; Androgen; B, corticosterone; CSP, Charcoal Stripped Plasma; DHEA, dehydroepiandrosterone; DHP, dihydroprogesterone; DHT, dihydrotestosterone; Dihydrotestosterone; E1, estrone; E2, estradiol; F, cortisol; IS, internal standard; LOD, lower limit of detection; LOQ, lower limit of quantification; Liquid chromatography–mass spectrometry; ME, matrix effect; MTBE, methyl tert-butyl ether; NMI, National Measurement Institute; P4, progesterone; P5, pregnenolone; S/N, signal-to-noise ratio; Steroidogenesis; T, testosterone; Testosterone
تواريخ الأحداث: Date Created: 20211129 Latest Revision: 20231108
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8620903
DOI: 10.1016/j.clinms.2018.12.003
PMID: 34841072
قاعدة البيانات: MEDLINE
الوصف
تدمد:2376-9998
DOI:10.1016/j.clinms.2018.12.003