دورية أكاديمية
أعرض في EDS

Alternative SARS-CoV-2 detection protocol from self-collected saliva for mass diagnosis and epidemiological studies in low-incoming regions.

التفاصيل البيبلوغرافية
العنوان: Alternative SARS-CoV-2 detection protocol from self-collected saliva for mass diagnosis and epidemiological studies in low-incoming regions.
المؤلفون: de Oliveira LPR; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil., Cabral AD; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil., Dos Santos Carmo AM; Santo André Regional Center, Adolfo Lutz Institute (ALI), Santo André, São Paulo, Brazil., Duran AF; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil., Fermino DM; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil., Veiga GRL; Laboratório de Análises Clínicas, Centro Universitário Saúde ABC (FMABC), Fundação ABC, Santo André, São Paulo, Brazil., da Costa Aguiar Alves B; Laboratório de Análises Clínicas, Centro Universitário Saúde ABC (FMABC), Fundação ABC, Santo André, São Paulo, Brazil., Santana CM; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil., Garcia FB; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil., Santos ES; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil., Jordão FT; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil., Siqueira AM; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil., de Campos IB; Santo André Regional Center, Adolfo Lutz Institute (ALI), Santo André, São Paulo, Brazil., Colpas DR; Santo André Regional Center, Adolfo Lutz Institute (ALI), Santo André, São Paulo, Brazil., Almeida FN; Centro de Engenharia, Modelagem e Ciências Sociais Aplicadas, Universidade Federal do ABC, São Bernardo do Campo, São Paulo, Brazil., Fonseca FLA; Laboratório de Análises Clínicas, Centro Universitário Saúde ABC (FMABC), Fundação ABC, Santo André, São Paulo, Brazil; Departamento de Ciências Farmacêuticas, Universidade Federal de São Paulo (UNIFESP), Diadema, São Paulo, Brazil., Sperança MA; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil. Electronic address: marcia.speranca@ufabc.edu.br.
المصدر: Journal of virological methods [J Virol Methods] 2022 Feb; Vol. 300, pp. 114382. Date of Electronic Publication: 2021 Nov 27.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980-
مواضيع طبية MeSH: COVID-19* , SARS-CoV-2*, Humans ; Nasopharynx ; Pandemics ; Prospective Studies ; RNA, Viral/genetics ; Saliva ; Specimen Handling
مستخلص: Until mass vaccination befalls, control of the new betacoronavirus-associated severe acute respiratory syndrome pandemic (SARS-CoV-2) is based on decreasing virus circulation by social distancing and blocking transmission foci after diagnosis. Globally adopted SARS-CoV-2 diagnostic criteria embrace viral RNA detection by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) on nasopharynx secretions, which requires healthcare facilities and specialized personnel for sample collection. To develop an alternative protocol, hydrophilic cotton as the material and saliva as the source for biological sample collection in qRT-PCR/RT-endpoint-PCR SARS-CoV-2 diagnostic methods prepared with local consumables were evaluated using 99 archived nasopharynx samples previously diagnosed as positive for SARS-CoV-2 and 111 prospective saliva samples pared with nasopharynx samples from patients attending the local reference ABC Medical School diagnostic laboratory. The kappa agreement coefficient between the SARS-CoV-2 qRT-PCR and RT-endpoint-PCR was k = 0.97 (95 % CI 0.92-1.00) and k = 0.90 (95 % CI 0.81-0.99), respectively, on SARS-CoV-2-positive archived samples, with the initial qRT-PCR C T under 25. The agreement coefficient of the SARS-CoV-2 alternative saliva diagnostic protocol, when used to test the paired nasopharynx samples, was k = 0.79 (95 % CI 0.56-1,00). These data support that the SARS-CoV-2 diagnostic assay based on self-collected saliva on cotton represents an alternative protocol for mass diagnosis and epidemiological studies in low-income regions.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
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فهرسة مساهمة: Keywords: Molecular diagnosis; RT-endpoint-PCR; SARS-CoV-2; Saliva; qRT-PCR
المشرفين على المادة: 0 (RNA, Viral)
تواريخ الأحداث: Date Created: 20211129 Date Completed: 20220107 Latest Revision: 20230523
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8626149
DOI: 10.1016/j.jviromet.2021.114382
PMID: 34843823
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0984
DOI:10.1016/j.jviromet.2021.114382