دورية أكاديمية
GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis.
| العنوان: | GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis. |
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| المؤلفون: | Gonçalves MFF; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Lacerda SMDSN; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Lara NLEM; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Oliveira CFA; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Figueiredo AFA; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Brener MRG; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Cavalcante MA; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Santos AK; Laboratory of Cardiac Signaling, Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Campolina-Silva GH; Center for Research and Development of Pharmaceuticals, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Costa VV; Center for Research and Development of Pharmaceuticals, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Santana ACC; Laboratory of Endocrinology and Metabolism, Department of Physiology and Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Lopes RA; Laboratory of Endocrinology and Metabolism, Department of Physiology and Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Szawka RE; Laboratory of Endocrinology and Metabolism, Department of Physiology and Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Costa GMJ; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: gmjc@ufmg.br. |
| المصدر: | Molecular and cellular endocrinology [Mol Cell Endocrinol] 2022 Feb 15; Vol. 542, pp. 111519. Date of Electronic Publication: 2021 Nov 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: North Holland Publishing Country of Publication: Ireland NLM ID: 7500844 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8057 (Electronic) Linking ISSN: 03037207 NLM ISO Abbreviation: Mol Cell Endocrinol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : North Holland Publishing Original Publication: Amsterdam, North-Holland. |
| مواضيع طبية MeSH: | Spermatogonia*/metabolism , Testis*/metabolism, Animals ; Leydig Cells/metabolism ; Male ; Mice ; Mutation/genetics ; Seminiferous Tubules ; Testosterone/metabolism |
| مستخلص: | GATA-1 is a transcription factor from the GATA family, which features zinc fingers for DNA binding. This protein was initially identified as a crucial regulator of blood cell differentiation, but it is currently known that the Gata-1 gene expression is not limited to this system. Although the testis is also a site of significant GATA-1 expression, its role in testicular cells remains considerably unexplored. In the present study, we evaluated the testicular morphophysiology of adult ΔdblGATA mice with a mutation in the GATA-1 protein. Regarding testicular histology, GATA-1 mutant mice exhibited few changes in the seminiferous tubules, particularly in germ cells. A high proportion of differentiated spermatogonia, an increased number of apoptotic pre-leptotene spermatocytes (Caspase-3-positive), and a high frequency of sperm head defects were observed in ΔdblGATA mice. The main differences were observed in the intertubular compartment, as ΔdblGATA mice showed several morphofunctional changes in Leydig cells. Reduced volume, increased number and down-regulation of steroidogenic enzymes were observed in ΔdblGATA Leydig cells. Moreover, the mutant animal showed lower serum testosterone concentration and high LH levels. These results are consistent with the phenotypic and biometric data of mutant mice, i.e., shorter anogenital index and reduced accessory sexual gland weight. In conclusion, our findings suggest that GATA-1 protein is an important factor for germ cell differentiation as well as for the steroidogenic activity in the testis. (Copyright © 2021 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Germ cell differentiation; Leydig cell; Testis; Testosterone; ΔdblGATA |
| المشرفين على المادة: | 3XMK78S47O (Testosterone) |
| تواريخ الأحداث: | Date Created: 20211129 Date Completed: 20220404 Latest Revision: 20220531 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.mce.2021.111519 |
| PMID: | 34843900 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-8057 |
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| DOI: | 10.1016/j.mce.2021.111519 |