Seedborne Cercospora beticola Can Initiate Cercospora Leaf Spot from Sugar Beet ( Beta vulgaris ) Fruit Tissue.

التفاصيل البيبلوغرافية
العنوان:	Seedborne Cercospora beticola Can Initiate Cercospora Leaf Spot from Sugar Beet ( Beta vulgaris ) Fruit Tissue.
المؤلفون:	Spanner R; Edward T. Schafer Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Fargo, ND, U.S.A.; Department of Plant Pathology, North Dakota State University, Fargo, ND, U.S.A., Neubauer J; Edward T. Schafer Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Fargo, ND, U.S.A., Heick TM; Institute for Agroecology, Aarhus University, Slagelse, Denmark., Grusak MA; Edward T. Schafer Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Fargo, ND, U.S.A., Hamilton O; Edward T. Schafer Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Fargo, ND, U.S.A.; Department of Plant Pathology, North Dakota State University, Fargo, ND, U.S.A., Rivera-Varas V; Department of Plant Pathology, North Dakota State University, Fargo, ND, U.S.A., de Jonge R; Plant-Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, The Netherlands., Pethybridge S; Plant Pathology & Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell AgriTech, Cornell University, Geneva, NY, U.S.A., Webb KM; Soil Management and Sugar Beet Research Unit, United States Department of Agriculture-Agricultural Research Service, Fort Collins, CO, U.S.A., Leubner-Metzger G; Department of Biological Sciences, Royal Holloway University of London, Egham, U.K., Secor GA; Department of Plant Pathology, North Dakota State University, Fargo, ND, U.S.A., Bolton MD; Edward T. Schafer Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Fargo, ND, U.S.A.; Department of Plant Pathology, North Dakota State University, Fargo, ND, U.S.A.
المصدر:	Phytopathology [Phytopathology] 2022 May; Vol. 112 (5), pp. 1016-1028. Date of Electronic Publication: 2022 Apr 06.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: American Phytopathological Society] Country of Publication: United States NLM ID: 9427222 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0031-949X (Print) Linking ISSN: 0031949X NLM ISO Abbreviation: Phytopathology Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: [St. Paul, Minn., etc., American Phytopathological Society]
مواضيع طبية MeSH:	Beta vulgaris/microbiology , Cercospora, Fruit ; Plant Diseases/microbiology ; Sugars ; Vegetables
مستخلص:	Cercospora leaf spot (CLS) is a globally important disease of sugar beet ( Beta vulgaris ) caused by the fungus Cercospora beticola . Long-distance movement of C. beticola has been indirectly evidenced in recent population genetic studies, suggesting potential dispersal via seed. Commercial sugar beet "seed" consists of the reproductive fruit (true seed surrounded by maternal pericarp tissue) coated in artificial pellet material. In this study, we confirmed the presence of viable C. beticola in sugar beet fruit for 10 of 37 tested seed lots. All isolates harbored the G143A mutation associated with quinone outside inhibitor resistance, and 32 of 38 isolates had reduced demethylation inhibitor sensitivity (EC 50 > 1 µg/ml). Planting of commercial sugar beet seed demonstrated the ability of seedborne inoculum to initiate CLS in sugar beet. C. beticola DNA was detected in DNA isolated from xylem sap, suggesting the vascular system is used to systemically colonize the host. We established nuclear ribosomal internal transcribed spacer region amplicon sequencing using the MinION platform to detect fungi in sugar beet fruit. Fungal sequences from 19 different genera were identified from 11 different sugar beet seed lots, but Fusarium , Alternaria , and Cercospora were consistently the three most dominant taxa, comprising an average of 93% relative read abundance over 11 seed lots. We also present evidence that C. beticola resides in the pericarp of sugar beet fruit rather than the true seed. The presence of seedborne inoculum should be considered when implementing integrated disease management strategies for CLS of sugar beet in the future.
فهرسة مساهمة:	Keywords: etiology; fungal pathogens; microbiome
المشرفين على المادة:	0 (Sugars)
SCR Organism:	Cercospora beticola
تواريخ الأحداث:	Date Created: 20211130 Date Completed: 20220428 Latest Revision: 20220428
رمز التحديث:	20240628
DOI:	10.1094/PHYTO-03-21-0113-R
PMID:	34844416
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	0031-949X
DOI:	10.1094/PHYTO-03-21-0113-R