(R)-(+)-Lasiodiplodin isolated from the endophytic fungus Sordaria tamaensis exhibits potent antimycobacterial and anti-inflammatory activities in vitro and in vivo: a dual approach for the treatment of severe pulmonary tuberculosis.

التفاصيل البيبلوغرافية
العنوان:	(R)-(+)-Lasiodiplodin isolated from the endophytic fungus Sordaria tamaensis exhibits potent antimycobacterial and anti-inflammatory activities in vitro and in vivo: a dual approach for the treatment of severe pulmonary tuberculosis.
المؤلفون:	Calixto SD; Laboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil.; Laboratório de Produtos Bioativos, Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Macaé, Rio de Janeiro, Brazil., Simão TLBV; Laboratório de Produtos Bioativos, Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Macaé, Rio de Janeiro, Brazil., de Almeida FM; Laboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil., Antunes SS; Laboratório Integrado de Computação Científica, Programa de Pós Graduação em Produtos Bioativos e Biociências, Universidade Federal do Rio de Janeiro, Campus Macaé, Macaé, Rio de Janeiro, Brazil., Romeiro NC; Laboratório Integrado de Computação Científica, Programa de Pós Graduação em Produtos Bioativos e Biociências, Universidade Federal do Rio de Janeiro, Campus Macaé, Macaé, Rio de Janeiro, Brazil., de Souza Borges W; Departamento de Química, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil., das Chagas FO; Instituto de Pesquisa de Produtos Naturais Walter Mors, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Seldin L; Laboratório de Genética Microbiana CCS, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Carvalho ECQ; Laboratório de Morfologia e Patologia Animal, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil., Andrioli WJ; Laboratório de Produtos Bioativos, Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Macaé, Rio de Janeiro, Brazil., Guimarães DO; Laboratório de Produtos Bioativos, Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Macaé, Rio de Janeiro, Brazil., Lasunskaia E; Laboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil., Muzitano MF; Laboratório de Produtos Bioativos, Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Macaé, Rio de Janeiro, Brazil.
المصدر:	The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2022 Mar 03; Vol. 74 (3), pp. 446-457.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Oxford University Press Country of Publication: England NLM ID: 0376363 Publication Model: Print Cited Medium: Internet ISSN: 2042-7158 (Electronic) Linking ISSN: 00223573 NLM ISO Abbreviation: J Pharm Pharmacol Subsets: MEDLINE
أسماء مطبوعة:	Publication: 2021- : [Oxford] : Oxford University Press Original Publication: London : Pharmaceutical Society of Great Britain
مواضيع طبية MeSH:	Anti-Inflammatory Agents/pharmacology , Antitubercular Agents/pharmacology , Sordariales/chemistry , Zearalenone/analogs & derivatives, Animals ; Anti-Inflammatory Agents/isolation & purification ; Antitubercular Agents/isolation & purification ; Caco-2 Cells ; Humans ; Inflammation/drug therapy ; Lipopolysaccharides ; Macrophages/drug effects ; Mice ; Mice, Inbred C57BL ; Mycobacterium tuberculosis/drug effects ; RAW 264.7 Cells ; Rubiaceae/microbiology ; Sordariales/isolation & purification ; Tuberculosis, Pulmonary/drug therapy ; Tuberculosis, Pulmonary/microbiology ; Zearalenone/isolation & purification ; Zearalenone/pharmacology
مستخلص:	Objectives: This study aimed to evaluate endophytic fungi isolated from Tocoyena bullata and Humiria balsamifera plant species for their antimycobacterial and anti-inflammatory activities, focusing on severe pulmonary tuberculosis cases which are often associated with exacerbated inflammation. Methods: Mycobacterium suspensions were incubated with the samples for 5 days. RAW 264.7 macrophages stimulated with LPS were also incubated with them for 24 h to assess the inhibition of inflammatory mediator production and cytotoxicity. C57BL/6 mice were infected with Mtb M299 and treated for 15 days with lasiodiplodin (Lasio). Key Findings: Endophytic fungus Sordaria tamaensis, obtained from T. bullata, was the most promising. Its ethanolic extract impaired mycobacterial growth with MIC50 (µg/ml): 1.5 ± 0.6 (BCG), 66.8 ± 0.1 (H37Rv) and 80.0 ± 0.1 (M299). (R)-(+)-Lasio showed MIC50 92.2 ± 1.8 µg/ml (M299). In addition, Lasio was able to inhibit NO, IL-1β and TNF-α production and was not cytotoxic for macrophages. M. tuberculosis-infected C57BL/6 animals treated by Lasio reduced the number of acid-fast bacilli, lung pathology, leucocyte influx and proinflammatory cytokine production in the lungs. The class IIa fructose 1,6-bisphosphate aldolase was the predicted hypothetical target of Lasio. Conclusions: (R)-(+)-Lasio stood out as a promising anti-TB compound, exhibiting anti-inflammatory and antimycobacterial effects, as well as low cytotoxicity. (© The Author(s) 2021. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
معلومات مُعتمدة:	Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
فهرسة مساهمة:	Keywords: endophytic fungus; inflammation; natural products; treatment; tuberculosis
المشرفين على المادة:	0 (Anti-Inflammatory Agents) 0 (Antitubercular Agents) 0 (Lipopolysaccharides) 0 (lasiodiplodin) 5W827M159J (Zearalenone)
SCR Organism:	Sordaria tamaensis
تواريخ الأحداث:	Date Created: 20211201 Date Completed: 20220316 Latest Revision: 20220316
رمز التحديث:	20231215
DOI:	10.1093/jpp/rgab165
PMID:	34850064
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	2042-7158
DOI:	10.1093/jpp/rgab165