دورية أكاديمية
HIF1A-induced heme oxygenase 1 promotes the survival of decidual stromal cells against excess heme-mediated oxidative stress.
| العنوان: | HIF1A-induced heme oxygenase 1 promotes the survival of decidual stromal cells against excess heme-mediated oxidative stress. |
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| المؤلفون: | Shen HH; Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China., Wang CJ; Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China., Zhang XY; Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China., Sheng YR; Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China., Yang SL; Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China., Zheng ZM; Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China., Shi JL; Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China., Qiu XM; Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China.; Clinical Research Center, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, People's Republic of China., Xie F; Medical Center of Diagnosis and Treatment for Cervical and Intrauterine Diseases, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, People's Republic of China., Li MQ; Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China.; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People's Republic of China.; Department of Obstetrics and Gynecology, Maternal and Child Health Hospital of Longgang District, Shenzhen, Guangdong Province, People's Republic of China. |
| المصدر: | Reproduction (Cambridge, England) [Reproduction] 2021 Dec 27; Vol. 163 (1), pp. 33-43. Date of Electronic Publication: 2021 Dec 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for the Society for Reproduction and Fertility by BioScientifica Country of Publication: England NLM ID: 100966036 Publication Model: Electronic Cited Medium: Internet ISSN: 1741-7899 (Electronic) Linking ISSN: 14701626 NLM ISO Abbreviation: Reproduction Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004->: Bristol, UK : Published for the Society for Reproduction and Fertility by BioScientifica Original Publication: Cambridge, UK : Journals of Reproduction and Fertility, Ltd. c2001- |
| مواضيع طبية MeSH: | Heme Oxygenase-1*/genetics , Heme Oxygenase-1*/metabolism , Heme Oxygenase-1*/pharmacology , Hydrogen Peroxide*/pharmacology, Apoptosis ; Cell Survival ; Heme ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/genetics ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; NF-E2-Related Factor 2/metabolism ; NF-E2-Related Factor 2/pharmacology ; Oxidative Stress ; Reactive Oxygen Species/metabolism ; Stromal Cells/metabolism |
| مستخلص: | Heme oxygenase 1 (HO-1, encoded by the HMOX1 gene) is the rate-limiting enzyme that catalyzes heme degradation, and it has been reported to exert antioxidative effects. Recently, decidualization has been reported to confer resistance to environmental stress signals, protecting against oxidative stress. However, the effects and regulatory mechanism of HO-1 in decidual stromal cells (DSCs) during early pregnancy remain unknown. Here, we verified that the levels of HO-1 and heme in DSCs are increased compared with those in endometrial stromal cells. Additionally, the upregulation of HIF1A expression led to increased HMOX1 expression in DSCs possibly via nuclear factor erythroid 2-related factor (encoded by the NFE2L2 gene). However, addition of the competitive HO-1 inhibitor zinc protoporphyrin IX resulted in an increase in HIF1A expression. Hydrogen peroxide (H2O2) induced the production of reactive oxygen species (ROS), decreased the cell viability of DSCs in vitro, and upregulated the level of heme. As an HO-1 inducer, cobalt protoporphyrin IX decreased ROS production and significantly reversed the inhibitory effect of H2O2 on cell viability. More importantly, patients with unexplained spontaneous abortion had low levels of HO-1 that were insufficient to protect against oxidative stress. This study suggests that the upregulation of HO-1 expression via HIF1A protects DSCs against excessive heme-mediated oxidative stress. Furthermore, the excessive oxidative stress injury and impaired viability of DSCs associated with decreased HO-1 expression should be associated with the occurrence and/or development of spontaneous abortion. |
| المشرفين على المادة: | 0 (HIF1A protein, human) 0 (Hypoxia-Inducible Factor 1, alpha Subunit) 0 (NF-E2-Related Factor 2) 0 (Reactive Oxygen Species) 42VZT0U6YR (Heme) BBX060AN9V (Hydrogen Peroxide) EC 1.14.14.18 (Heme Oxygenase-1) |
| تواريخ الأحداث: | Date Created: 20211207 Date Completed: 20220325 Latest Revision: 20220325 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1530/REP-21-0314 |
| PMID: | 34874275 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1741-7899 |
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| DOI: | 10.1530/REP-21-0314 |