دورية أكاديمية
L-carnitine added to post-thawed semen acts as an antioxidant and a stimulator of equine sperm metabolism.
العنوان: | L-carnitine added to post-thawed semen acts as an antioxidant and a stimulator of equine sperm metabolism. |
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المؤلفون: | Lagares MA; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., da Silva GC; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Cortes SF; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Moreira FHM; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Neves FCD; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Alves NC; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Viegas RN; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Diniz TF; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Lemos VS; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., de Rezende ASC; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Freitas MM; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Stahlberg R; Faculty of Veterinary Medicine, Pontifical Catholic University (PUC- Minas) of Minas Gerais, Betim, Brazil., Nicolino RR; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil. |
المصدر: | Andrologia [Andrologia] 2022 Apr; Vol. 54 (3), pp. e14338. Date of Electronic Publication: 2021 Dec 08. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Blackwell Pub Country of Publication: Germany NLM ID: 0423506 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0272 (Electronic) Linking ISSN: 03034569 NLM ISO Abbreviation: Andrologia Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Original Publication: Berlin, Grosse |
مواضيع طبية MeSH: | Semen* , Semen Preservation*/veterinary, Animals ; Antioxidants/pharmacology ; Carnitine/pharmacology ; Cryopreservation/veterinary ; Female ; Fertility ; Horses ; Insemination, Artificial/veterinary ; Male ; Pregnancy ; Sperm Motility ; Spermatozoa |
مستخلص: | The objective of this study was to enhance the in vitro sperm quality and in vivo fertility of frozen-thawed equine semen by the addition of l-carnitine (LC) to post-thawed semen. Different concentrations of LC were added to thawed samples to obtain four treatments control and 0.5, 1 and 2 mM LC. In the in vitro experiments, sperm motility and kinematics, membrane integrity and intracellular calcium ion concentration ([Ca 2+ ] (© 2021 Wiley-VCH GmbH.) |
References: | Abd-Allah, A. R. , Helal, G. K. , Al-Yahya, A. A. , Aleisa, A. M. , Al-Rejaie, S. S. , & Al-Bakheet, S. A. (2009). Pro-inflammatory and oxidative stress pathways which compromise sperm motility and survival may be altered by L-carnitine. Oxidative Medicine and Cellular Longevity, 2, 73-81. https://doi.org/10.4161/oxim.2.2.8177. Agarwal, A. , & Said, T. M. (2004). Carnitines and male infertility. Reproductive BioMedicine Online, 4, 376-384. https://doi.org/10.1016/s1472-6483(10)60920-0. Aitken, R. J. , & Drevet, J. R. (2020). The importance of oxidative stress in determining the functionality of mammalian spermatozoa: A two-edged sword. Antioxidants, 9, 111. https://doi.org/10.3390/antiox9020111. Alasmari, W. , Barratt, C. L. R. , Publicover, S. J. , Whalley, K. M. , Foster, E. , Kay, V. , Silva, S. M. & Oxenham, S. K. (2013). The clinical significance of calcium signalling pathways mediating human sperm hyperactivation. Human Reprod, 28, 866-876. https://doi.org/10.1093/humrep/des467. Aliabadi, E. , Jahanshahi, S. , Talaei-Khozani, T. , & Banaei, M. (2017). Comparison and evaluation of capacitation and acrosomal reaction in freeze-thawed human ejaculated spermatozoa treated with L-carnitine and pentoxifylline. Andrologia, 50, e12845. https://doi.org/10.1111/and.12845. Amidi, F. , Pazhohan Nashtaei, M. S. , Khodarahmian, M. , & Nekoonam, S. (2016). The role of antioxidants in sperm freezing: a review. Cell Tissue Bank, 17, 745-756. https://doi.org/10.1007/s10561-016-9566-5. Balercia, G. , Moretti, S. , Vignini, A. , Magagnini, M. , Mantero, F. , Boscaro, M. , Riccardo Lamonica, G. , & Mazzanti, L. (2004). Role of nitric oxide concentrations on human sperm motility. Journal of Andrology, 2, 245-249. https://doi.org/10.1002/j.1939-4640.2004.tb02784.x. Banihani, S. A. , Bayachou, M. , & Alzoubi, K. (2015). Carnitine is a calcium chelator: a reason for its useful and toxic effects in biological systems. Journal of Basic and Clinical Physiology and Pharmacology, 26, 141-145. https://doi.org/10.1515/jbcpp-2014-0016. Banihani, S. , Sharma, R. , Bayachou, M. , Sabanegh, E. , & Agarwal, A. (2012). Human sperm DNA oxidation, motility and viability in the presence of l-carnitine during in vitro incubation and centrifugation. Andrologia, 44, 505-512. https://doi.org/10.1111/j.1439-0272.2011.01216.x. Beckman, J. S. , & Koppenol, W. H. (1996). Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and the ugly. American Journal of Physiology, 271, C1424-C1437. https://doi.org/10.1152/ajpcell.1996.271.5.C1424. Bøhmer, T. , & Johansen, L. (1978). Inhibition of sperm maturation through intervention of the carnitine system. International Journal of Andrology, 2, 565-569. https://doi.org/10.1111/j.1365-2605.1978.tb00509.x. Bremer, J. (1983). Carnitine-metabolism and functions. Physiological Reviews, 63, 1420-1480. https://doi.org/10.1152/physrev.1983.63.4.1420. Brinsko, S. P. , Crockett, E. C. , & Squires, E. L. (2000). Effect of centrifugation and partial removal of seminal plasma on equine spermatozoal motility after cooling and storage. Theriogenology, 54, 12-136. https://doi.org/10.1016/S0093-691X(00)00331-9. Brown, G. C. (1999). Nitric oxide and mitochondrial respiration. Biochimica Et Biophysica, Acta, 1411, 351-369. https://doi.org/10.1016/s0005-2728(99)00025-0. Calvin, J. , & Tubbs, P. K. (1976). A carnitine: acetylcarnitine exchange system in spermatozoa. Journal of Reproduction and Fertility, 48, 417-420. https://doi.org/10.1530/jrf.0.0480417. Casillas, E. R. (1972). The distribution of carnitine in male reproductive tissues and its effect on palmitate oxidation by spermatozoal particles. Biochimica Et Biophysica Acta, 280, 545-551. https://doi.org/10.1016/0005-2760(72)90134-8. Costa, M. , Canale, D. , Filicori, M. , D'Lddio, S. , & Lenzi, A. (1994). L-carnitine in idiopathic asthenozoospermia: A multicenter study. Andrologia, 26, 155-159. https://doi.org/10.1111/j.1439-0272.1994.tb00780.x. Costello, S. , Michelangeli, F. , Nash, K. , Lefievre, L. , Morris, J. , Machado-Oliveira, G. , Barratt, C. , Kirkman-Brown, J. , & Publicover, S. (2009). Ca2C-stores in sperm: their identities and functions. Reproduction, 138, 425-437. https://doi.org/10.1530/REP-09-0134. Darszon, A. , Nishigaki, T. , Beltran, C. , & Trevino, C. L. (2011). Calcium channels in the development, maturation, and function of spermatozoa. Physiological Reviews, 91, 1305-1355. https://doi.org/10.1152/physrev.00028.2010. Davila, M. P. , Martin Muñoz, P. , Gallardo Bolaños, J. M. , Stout, T. A. E. , Gadella, B. M. , Tapia, J. A. , Balao da Silva, C. C. , Ferrusola, C. O. , & Peña, F. J. (2016). Mitochondrial ATP is required for the maintenance of membrane integrity in stallion spermatozoa, whereas motility requires both glycolysis and oxidative phosphorylation. Reproduction, 152, 683-694. https://doi.org/10.1530/REP-16-0409. Deana, R. , Rigoni, F. , Bonaga, O. , Andrigheuo, I. , Bonsembiante, M. , & Siliprandi, N. (1984). Effect of carnitine on functions of bovine spermatozoa. Proc 10th Int Congr Animal Reproduction Artificial Insemination 258-61. Deana, R. , Rigoni, F. , Francesconi, M. , Cavallini, L. , Arslan, P. , & Siliprandi, N. (1989). Effect of l-carnitine and l-aminocarnitine on calcium transport, motility, and enzyme release from ejaculated bovine spermatozoa. Biology of Reproduction, 41, 949-955. https://doi.org/10.1095/biolreprod41.5.949. Ferrusola, O. C. , Fernandez, L. G. , Garcıa, B. M. , Salazar-Sandoval, C. , Rodrıguez, A. M. , Martinez, H. R. , Tapia, J. A. , & Peña, F. J. (2009). Effect of cryopreservation on nitric oxide production by stallion Spermatozoa. Biology of Reproduction, 81, 1106-1111. https://doi.org/10.1095/biolreprod.109.078220. Franco, J. S. V. , Chaveiro, A. , & Silva, F. M. (2014). Effect of freezing rates and supplementation of α-tocopherol in the freezing extender in equine sperm cryosurvival. Journal of Equine Veterinary Science, 34, 992-997. https://doi.org/10.1016/j.jevs.2014.05.007. Gibb, Z. , Butler, T. J. , Morris, L. H. A. , Maxwell, W. M. C. , & Grupen, C. G. (2013). Quercetin improves the postthaw characteristics of cryopreserved sex-sorted and nonsorted stallion sperm. Theriogenology, 79, 1001-1009. https://doi.org/10.1016/j.theriogenology.2012.06.032. Gibb, Z. , Lambourne, S. R. , & Aitken, R. J. (2014). The paradoxical relationship between stallion fertility and oxidative stress. Biology of Reproduction, 91, 1-10. https://doi.org/10.1095/biolreprod.114.118539. Gibb, Z. , Lambourne, S. R. , Quadrelli, J. , Smith, N. D. , & Aitken, R. J. (2015). L-carnitine and pyruvate are prosurvival factors during the storage of stallion spermatozoa at room temperature. Biology of Reproduction, 93, 1-9. https://doi.org/10.1095/biolreprod.115.131326. Golan, R. , Weissenberg, R. , & Lewin, L. M. (1984). Carnitine and acetylcarnitine in motile and immotile human spermatozoa. International Journal of Andrology, 7, 484-494. https://doi.org/10.1111/j.1365-2605.1984.tb00805.x. Green, L. C. , Wagner, D. A. , & Glogowski, J. (1982). Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Analytical Biochemistry, 126, 131-138. https://doi.org/10.1016/0003-2697(82)90118-x. Gülçin, I. (2006). Antioxidant and antiradical activities of l-carnitine. Life Sciences, 78, 803-811. https://doi.org/10.1016/j.lfs.2005.05.103. Hamilton, D. W. , & Olson, G. E. (1976). Effects of carnitine on oxygen uptake and utilization of (U-14C) palmitate by ejaculated bull spermatozoa. Journal of Reproduction and Fertility, 46, 195-202. https://doi.org/10.1530/jrf.0.0460195. Harrison, R. A. , & Vickers, S. E. (1990). Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. Journal of Reproduction and Fertility, 88, 343-352. https://doi.org/10.1530/jrf.0.0880343. Herrero, M. B. , Chatterjee, S. , Lefievre, L. , De Lamirande, E. , & Gagnon, C. (2000). Nitric oxide interacts with the cAMP pathway to modulate capacitation of human spermatozoa. Free Radical Biology and Medicine, 29, 522-536. https://doi.org/10.1016/s0891-5849(00)00339-7. Hinton, B. T. , Brooks, D. E. , Dott, H. M. , & Setchell, B. O. (1981). Effects of carnitine and some other related compounds on the motility of rat spermatozoa from the caput epididymis. Journal of Reproduction and Fertility, 61, 59-64. https://doi.org/10.1530/jrf.0.0610059. Ho, H. C. , & Suarez, S. S. (2003). Characterization of the intracellular calcium store at the base of the sperm flagellum that regulates hyperactivated motility. Biology of Reproduction, 68, 1590-1596. https://doi.org/10.1095/biolreprod.102.011320. Jeulin, C. , & Lewin, L. M. (1996). Role of free L-carnitine and acetyl-L-carnitine in post-gonadal maturation of mammalian spermatozoa. Human Reproduction Update, 2, 87-102. https://doi.org/10.1093/humupd/2.2.87. Katila, T. (2012). Post-mating inflammatory responses of the uterus. Reproduction in Domestic Animals, 47, 31-41. https://doi.org/10.1111/j.1439-0531.2012.02120.x. Kenney, R. M. , Bergman, R. V. , Cooper, W. L. , & Morse, G. (1975). Minimal contamination techniques for breeding mares: techniques and preliminary findings. Proceedings of American Association of Equine Practitioners, 21, 327-349. Koppenol, W. H. , Moreno, J. J. , Pryor, W. A. , Ischiropoulos, H. , & Beckman, J. S. (1992). Peroxynitrite, a cloaked oxidant formed by nitric oxide and superoxide. Chemical Research in Toxicology, 5, 834-842. https://doi.org/10.1021/tx00030a017. Lagares, M. A. , Meirelles, L. S. , Wald, V. B. , Gregory, R. M. , & Mattos, R. C. (2000). Efeito de diferentes diluidores sobre a membrana plasmática do espermatozoide equino e fertilidade do sêmen resfriado. Revista Brasileira De Ciência Veterinária, 7, 153-156. https://doi.org/10.4322/rbcv.2015.202. Lagares, M. A. , Silva, G. C. , Cortes, S. F. , Luz, S. B. , Resende, A. C. , Alves, N. C. , Wenceslau, R. R. , & Stahlberg, R. (2020). Does coenzyme Q10 exert antioxidant effect on frozen equine sperm? Journal of Equine Veterinary Science, 88, 102964. https://doi.org/10.1016/j.jevs.2020.102964. Leemans, B. , Stout, T. A. E. , De Schauwer, C. , Hera, S. , Nelis, H. , Hoogewijs, M. , Van Soom, A. , & Gadella, B. M. (2019). Update on mammalian sperm capacitation: how much does the horse differ from other species? Reproduction, 157, R181-R197. https://doi.org/10.1530/REP-18-0541. Lewis, S. E. M. , Donnelly, E. T. , Sterling, E. S. L. , Kennedy, M. S. , Thompson, W. , & Chakravarthy, U. (1996). Nitric oxide synthase and nitrite production in human spermatozoa: evidence that endogenous nitric oxide is beneficial for sperm motility. Molecular Human Reproduction, 11, 873-878. https://doi.org/10.1093/molehr/2.11.873. Martins, H. S. , Silva, G. C. , Cortes, S. F. , Paes, F. O. , Martins Filho, A. O. , Araújo, M. S. S. , Stahlberg, R. , & Lagares, M. A. (2018). Lactoferrin increases sperm membrane functionality of frozen equine semen. Reproduction in Domestic Animals, 53, 617-623. https://doi.org/10.1111/rda.13148. Mayorga-Torres, B. J. M. , Camargo, M. , Cadavid, Á. P. , du Plessis, S. S. , & Cardona Maya, W. D. (2017). Are oxidative stress markers associated with unexplained male infertility? Andrologia, 49, 12659. https://doi.org/10.1111/and.12659. Michaut, M. , Tomes, C. N. , de Blas, G. , Yunes, R. , & Mayorga, L. S. (2000). Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and 𝑁-ethylmaleimide-sensitive factor. Proceedings of the National Academy of Sciences of the United States of America, 97, 9996-10001. Morielli, T. , & O’Flaherty, C. (2015). Oxidative stress impairs function and increases redox protein modifications in human spermatozoa. Reproduction, 149, 113-123. https://doi.org/10.1530/REP-14-0240. Nourooz-Zadeh, J. , Tajaddini-Sarmadi, J. , & Wolff, S. P. (1994). Measurement of plasma hydroperoxide concentrations by the ferrous oxidation-xylenol orange assay in conjunction with triphenylphosphine. Analytical Biochemistry, 220, 403-409. https://doi.org/10.1006/abio.1994.1357. Nowicka-Bauer, K. , & Nixon, B. (2020). Molecular changes induced by oxidative stress that impair human sperm motility. Antioxidants, 9, 134. https://doi.org/10.3390/antiox9020134. Pillet, E. , Batellier, F. , Duchamp, G. , Furstoss, V. , Le Vern, Y. , Kerboeuf, D. , Vidament, M. , & Magistrini, M. (2008). Freezing stallion semen in INRA96-based extender improves fertility rates in comparison with INRA82. Dairy Science Technology, 88, 257-265. https://doi.org/10.1051/dst:2008002. Samper, J. C. (1997). Ultrasonographic appearance and the pattern of uterine Edema to time ovulation in mares. Proceedings of the 43rd Annual Convention of the American Association of Equine Practitioners Phoenix, Arizona 189-191. Sánchez-Cárdenas, C. , Servín-Vences, M. R. , José, O. , Treviño, C. L. , Hernández-Cruz, A. , & Darszon, A. (2014). Acrosome reaction and Ca 2+ imaging in single human spermatozoa: New regulatory roles of [Ca2+]i. Biology of Reproduction, 91, 67. https://doi.org/10.1095/biolreprod.114.119768. Sariozkan, S. , Ozdamar, S. , Turk, G. , Canturk, F. , & Yay, A. (2014). In vitro effects of L-carnitine and glutamine on motility, acrosomal abnormality, and plasma integrity of rabbit sperm during liquid- storage. Cryobiology, 68, 349-353. https://doi.org/10.1016/j.cryobiol.2014.04.006. Stradaioli, G. , Sylla, L. , Zelli, R. , Chiodi, P. , & Monaci, M. (2004). Effect of L-carnitine administration on the seminal characteristics of oligoasthenospermic stallions. Theriogenology, 62, 761-777. https://doi.org/10.1016/j.theriogenology.2003.11.018. Storey, B.T. , & Keyhani, E. (1974). Energy metabolism of spermatozoa. II. Comparison of pyruvate and fatty acid oxidation by mitochondria of rabbit epididymal spermatozoa. Fertility & Sterility, 25, 857-864. http://doi.org/10.1016/s0015-0282(16)40692-8. Suarez, S. S. (2008). Control of hyperactivation in sperm. Human Reproduction Update, 14, 647-657. https://doi.org/10.1093/humupd/dmn029. Surai, P. F. (2015). Carnitine Enigma: From antioxidant action to vitagene regulation part 2. Transcription factors and practical applications. Journal of Veterinary Science & Medicine, 3(17), https://doi.org/10.13188/2325-4645.1000018. Vignini, A. , Nanetti, L. , Buldreghini, E. , Moroni, C. , Ricciardo-Lamonina, G. , Mantero, F. , Boscaro, M. , Mazzanti, L. , & Balercia, G. (2006). The production of peroxynitrite by human spermatozoa may affect sperm motility through the formation of protein nitrotyrosine. Fertility and Sterility, 85, 947-953. https://doi.org/10.1016/j.fertnstert.2005.09.027. Yoshimoto, T. , Nakamura, S. , Yamauchi, S. , Muto, N. , Nakada, T. , Ashizawa, K. , & Tatemoto, H. (2008). Improvement of the post thaw qualities of Okinawan native pig spermatozoa frozen in an extender supplemented with ascorbic acid 2-O-aglucoside. Cryobiology, 57, 30-36. https://doi.org/10.1016/j.cryobiol.2008.05.002. |
معلومات مُعتمدة: | 1690817/2017-0487 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
فهرسة مساهمة: | Keywords: cryopreservation; frozen semen; intracellular calcium; nitrite; stallion |
المشرفين على المادة: | 0 (Antioxidants) S7UI8SM58A (Carnitine) |
تواريخ الأحداث: | Date Created: 20211208 Date Completed: 20220309 Latest Revision: 20220309 |
رمز التحديث: | 20231215 |
DOI: | 10.1111/and.14338 |
PMID: | 34878673 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1439-0272 |
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DOI: | 10.1111/and.14338 |