تقرير
Molecular investigations reveal bitter gourd crop is more susceptible to tomato leaf curl New Delhi virus infection in diverse crop cultivation practices.
| العنوان: | Molecular investigations reveal bitter gourd crop is more susceptible to tomato leaf curl New Delhi virus infection in diverse crop cultivation practices. |
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| المؤلفون: | Kiran GVNSM; Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore, 560065 Karnataka India., Nagaraju N; Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore, 560065 Karnataka India., Girish TR; Sea6 Energy Private Limited, C-Camp, NCBS, GKVK, Bangalore, India., Ashwini BN; Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore, 560065 Karnataka India. |
| المصدر: | 3 Biotech [3 Biotech] 2021 Dec; Vol. 11 (12), pp. 500. Date of Electronic Publication: 2021 Nov 17. |
| نوع المنشور: | Case Reports |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 101565857 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2190-572X (Print) Linking ISSN: 21905738 NLM ISO Abbreviation: 3 Biotech Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin : Springer |
| مستخلص: | Small- and medium-scale farmer's typically follow polyculture or diverse crop cultivation. However, cultivation of diverse crops in small area can cause cross infection leading to disease spreading across crops. A microplot-based field study was conducted to understand the disease susceptibility and disease mobility across various crops, including tomato, chilli, mungbean, and bitter gourd. The mungbean yellow mosaic virus (MYMV) incidence was noted first in the mungbean crop followed by tomato leaf curl New Delhi virus (ToLCNDV) in tomato and chilli leaf curl virus (ChLCV) in chilli crop. Interestingly, bitter gourd crop was infected lastly with symptoms including yellow and green mottling, severe leaf curling, and stunted growth. However, in bitter gourd crop symptoms, like typical leaf curl virus, could not be conclusively related to a certain type of begomovirus. Molecular diagnosis using begomovirus specific deng primers and coat protein (CP) gene primers specific to begomovirus species revealed the presence of ToLCNDV in bitter gourd samples. The phylogenetic analysis of CP gene sequences revealed 98 per cent nucleotide identity with ToLCNDV. Further cross infectivity assays confirmed the transmission of ToLCNDV from tomato to bitter gourd and vice versa. The cryptic species of whiteflies isolated from the bitter gourd fields were sequence confirmed to belong to Asia-I genetic group that were reported to transmit ToLCNDV previously. Overall, our study suggests the vulnerability of bitter gourd crop for ToLCNDV infection when cultivated by the side of tomato plots. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-021-02975-6. Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest in the publication. (© King Abdulaziz City for Science and Technology 2021.) |
| References: | 3 Biotech. 2017 Oct;7(5):331. (PMID: 28955628) Biotechniques. 1991 Apr;10(4):506-13. (PMID: 1867860) Arch Virol. 2019 Sep;164(9):2417-2429. (PMID: 31187277) Appl Microbiol Biotechnol. 2013 Jun;97(12):5457-71. (PMID: 23306645) 3 Biotech. 2020 Jun;10(6):282. (PMID: 32550101) Mol Ecol. 1999 Oct;8(10):1683-91. (PMID: 10583831) Arch Virol. 2015 Jun;160(6):1499-509. (PMID: 25894479) Phytopathology. 2020 Jan;110(1):10-17. (PMID: 31544592) Proc Natl Acad Sci U S A. 1990 Mar;87(5):1633-7. (PMID: 2308926) Plant Pathol J. 2016 Jun;32(3):201-8. (PMID: 27298595) Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. (PMID: 29722887) Proc Natl Acad Sci U S A. 2009 May 26;106(21):8778-83. (PMID: 19423673) Viruses. 2016 Nov 10;8(11):. (PMID: 27834936) Phytopathology. 1999 Nov;89(11):1038-43. (PMID: 18944659) J Gen Virol. 2017 Feb;98(2):131-133. (PMID: 28284245) |
| فهرسة مساهمة: | Keywords: Bitter gourd; Cross infectivity; ToLCNDV; Whitefly |
| تواريخ الأحداث: | Date Created: 20211209 Latest Revision: 20221202 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8595444 |
| DOI: | 10.1007/s13205-021-02975-6 |
| PMID: | 34881163 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2190-572X |
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| DOI: | 10.1007/s13205-021-02975-6 |