دورية أكاديمية
أعرض في EDS

Diagnosis of Taenia solium infections based on "mail order" RNA-sequencing of single tapeworm egg isolates from stool samples.

التفاصيل البيبلوغرافية
العنوان: Diagnosis of Taenia solium infections based on "mail order" RNA-sequencing of single tapeworm egg isolates from stool samples.
المؤلفون: Sadlowski H; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany., Schmidt V; Department of Neurology, Centre for Global Health, Klinikum rechts der Isar, Technical University Munich (TUM), Munich, Germany.; Centre for Global Health, Institute of Health and Society, University of Oslo, Oslo, Norway., Hiss J; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany., Kuehn JA; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany., Schneider CG; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany., Zulu G; School of Veterinary Medicine, University of Zambia, Lusaka, Zambia., Hachangu A; School of Veterinary Medicine, University of Zambia, Lusaka, Zambia., Sikasunge CS; School of Veterinary Medicine, University of Zambia, Lusaka, Zambia., Mwape KE; School of Veterinary Medicine, University of Zambia, Lusaka, Zambia., Winkler AS; Department of Neurology, Centre for Global Health, Klinikum rechts der Isar, Technical University Munich (TUM), Munich, Germany.; Centre for Global Health, Institute of Health and Society, University of Oslo, Oslo, Norway., Schuelke M; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany.; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Neuropediatrics, Berlin, Germany.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2021 Dec 10; Vol. 15 (12), pp. e0009787. Date of Electronic Publication: 2021 Dec 10 (Print Publication: 2021).
نوع المنشور: Evaluation Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Feces/*parasitology , Sequence Analysis, RNA/*methods , Taenia solium/*genetics , Taeniasis/*diagnosis , Taeniasis/*parasitology, Animals ; DNA, Mitochondrial/genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Ovum/chemistry ; Postal Service ; RNA, Helminth/genetics ; Species Specificity ; Taenia solium/classification ; Taenia solium/isolation & purification
مستخلص: Combined community health programs aiming at health education, preventive anti-parasitic chemotherapy, and vaccination of pigs have proven their potential to regionally reduce and even eliminate Taenia solium infections that are associated with a high risk of neurological disease through ingestion of T. solium eggs. Yet it remains challenging to target T. solium endemic regions precisely or to make exact diagnoses in individual patients. One major reason is that the widely available stool microscopy may identify Taenia ssp. eggs in stool samples as such, but fails to distinguish between invasive (T. solium) and less invasive Taenia (T. saginata, T. asiatica, and T. hydatigena) species. The identification of Taenia ssp. eggs in routine stool samples often prompts a time-consuming and frequently unsuccessful epidemiologic workup in remote villages far away from a diagnostic laboratory. Here we present "mail order" single egg RNA-sequencing, a new method allowing the identification of the exact Taenia ssp. based on a few eggs found in routine diagnostic stool samples. We provide first T. solium transcriptome data, which show extremely high mitochondrial DNA (mtDNA) transcript counts that can be used for subspecies classification. "Mail order" RNA-sequencing can be administered by health personnel equipped with basic laboratory tools such as a microscope, a Bunsen burner, and access to an international post office for shipment of samples to a next generation sequencing facility. Our suggested workflow combines traditional stool microscopy, RNA-extraction from single Taenia eggs with mitochondrial RNA-sequencing, followed by bioinformatic processing with a basic laptop computer. The workflow could help to better target preventive healthcare measures and improve diagnostic specificity in individual patients based on incidental findings of Taenia ssp. eggs in diagnostic laboratories with limited resources.
Competing Interests: The authors have declared that no competing interests exist.
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المشرفين على المادة: 0 (DNA, Mitochondrial)
0 (RNA, Helminth)
تواريخ الأحداث: Date Created: 20211210 Date Completed: 20220218 Latest Revision: 20220218
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8694474
DOI: 10.1371/journal.pntd.0009787
PMID: 34890398
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0009787