دورية أكاديمية
Diagnosis of Taenia solium infections based on "mail order" RNA-sequencing of single tapeworm egg isolates from stool samples.
العنوان: | Diagnosis of Taenia solium infections based on "mail order" RNA-sequencing of single tapeworm egg isolates from stool samples. |
---|---|
المؤلفون: | Sadlowski H; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany., Schmidt V; Department of Neurology, Centre for Global Health, Klinikum rechts der Isar, Technical University Munich (TUM), Munich, Germany.; Centre for Global Health, Institute of Health and Society, University of Oslo, Oslo, Norway., Hiss J; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany., Kuehn JA; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany., Schneider CG; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany., Zulu G; School of Veterinary Medicine, University of Zambia, Lusaka, Zambia., Hachangu A; School of Veterinary Medicine, University of Zambia, Lusaka, Zambia., Sikasunge CS; School of Veterinary Medicine, University of Zambia, Lusaka, Zambia., Mwape KE; School of Veterinary Medicine, University of Zambia, Lusaka, Zambia., Winkler AS; Department of Neurology, Centre for Global Health, Klinikum rechts der Isar, Technical University Munich (TUM), Munich, Germany.; Centre for Global Health, Institute of Health and Society, University of Oslo, Oslo, Norway., Schuelke M; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Berlin, Germany.; Charité-Universitätsmedizin Berlin, corporate member of the Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Neuropediatrics, Berlin, Germany. |
المصدر: | PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2021 Dec 10; Vol. 15 (12), pp. e0009787. Date of Electronic Publication: 2021 Dec 10 (Print Publication: 2021). |
نوع المنشور: | Evaluation Study; Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
مواضيع طبية MeSH: | Feces/*parasitology , Sequence Analysis, RNA/*methods , Taenia solium/*genetics , Taeniasis/*diagnosis , Taeniasis/*parasitology, Animals ; DNA, Mitochondrial/genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Ovum/chemistry ; Postal Service ; RNA, Helminth/genetics ; Species Specificity ; Taenia solium/classification ; Taenia solium/isolation & purification |
مستخلص: | Combined community health programs aiming at health education, preventive anti-parasitic chemotherapy, and vaccination of pigs have proven their potential to regionally reduce and even eliminate Taenia solium infections that are associated with a high risk of neurological disease through ingestion of T. solium eggs. Yet it remains challenging to target T. solium endemic regions precisely or to make exact diagnoses in individual patients. One major reason is that the widely available stool microscopy may identify Taenia ssp. eggs in stool samples as such, but fails to distinguish between invasive (T. solium) and less invasive Taenia (T. saginata, T. asiatica, and T. hydatigena) species. The identification of Taenia ssp. eggs in routine stool samples often prompts a time-consuming and frequently unsuccessful epidemiologic workup in remote villages far away from a diagnostic laboratory. Here we present "mail order" single egg RNA-sequencing, a new method allowing the identification of the exact Taenia ssp. based on a few eggs found in routine diagnostic stool samples. We provide first T. solium transcriptome data, which show extremely high mitochondrial DNA (mtDNA) transcript counts that can be used for subspecies classification. "Mail order" RNA-sequencing can be administered by health personnel equipped with basic laboratory tools such as a microscope, a Bunsen burner, and access to an international post office for shipment of samples to a next generation sequencing facility. Our suggested workflow combines traditional stool microscopy, RNA-extraction from single Taenia eggs with mitochondrial RNA-sequencing, followed by bioinformatic processing with a basic laptop computer. The workflow could help to better target preventive healthcare measures and improve diagnostic specificity in individual patients based on incidental findings of Taenia ssp. eggs in diagnostic laboratories with limited resources. Competing Interests: The authors have declared that no competing interests exist. |
References: | Nat Protoc. 2016 Sep;11(9):1650-67. (PMID: 27560171) PLoS Negl Trop Dis. 2020 Jan 16;14(1):e0007873. (PMID: 31945055) Mol Syst Biol. 2011 Oct 11;7:539. (PMID: 21988835) PLoS Negl Trop Dis. 2012;6(3):e1594. (PMID: 22479664) Lancet. 2004 Jun 12;363(9425):1999; author reply 1999. (PMID: 15194265) Pathog Glob Health. 2012 Sep;106(5):261-74. (PMID: 23265550) Epilepsia. 2012 Dec;53(12):2194-202. (PMID: 23148555) J Clin Microbiol. 2008 Jan;46(1):286-9. (PMID: 17989190) RNA. 2020 Aug;26(8):903-909. (PMID: 32284352) Lancet. 1993 Sep 18;342(8873):748. (PMID: 8103859) Parasit Vectors. 2021 Jan 29;14(1):88. (PMID: 33514421) Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886) Lancet. 2004 Mar 20;363(9413):949-50. (PMID: 15043964) Elife. 2020 Mar 06;9:. (PMID: 32142406) Epidemiol Infect. 2019 Jan;147:e100. (PMID: 30869032) Genome Biol. 2019 Apr 9;20(1):70. (PMID: 30961669) Neurology. 2000 Oct 10;55(7):1062. (PMID: 11061275) Nature. 2013 Apr 04;496(7443):57-63. (PMID: 23485966) Korean J Parasitol. 2013 Feb;51(1):43-9. (PMID: 23467388) Nat Protoc. 2014 Jan;9(1):171-81. (PMID: 24385147) Am J Trop Med Hyg. 2003 Mar;68(3):268-75. (PMID: 12685628) Bioinformatics. 2019 Jul 15;35(14):2489-2491. (PMID: 30520945) PLoS Negl Trop Dis. 2019 Jan 24;13(1):e0007101. (PMID: 30677038) J Clin Microbiol. 2000 Jan;38(1):133-7. (PMID: 10618076) N Engl J Med. 2016 Sep 22;375(12):1196-7. (PMID: 27653579) Parasit Vectors. 2021 May 1;14(1):233. (PMID: 33933158) PLoS Negl Trop Dis. 2018 Feb 8;12(2):e0006260. (PMID: 29420601) PLoS Negl Trop Dis. 2017 Sep 11;11(9):e0005875. (PMID: 28892472) Nat Biotechnol. 2011 Jan;29(1):24-6. (PMID: 21221095) PLoS Negl Trop Dis. 2010 Nov 02;4(11):e870. (PMID: 21072231) PLoS Negl Trop Dis. 2017 Jul 7;11(7):e0005743. (PMID: 28686662) Cold Spring Harb Perspect Med. 2016 Nov 1;6(11):. (PMID: 27638353) Lancet. 2003 Aug 16;362(9383):547-56. (PMID: 12932389) Acta Trop. 2020 Nov;211:105628. (PMID: 32659282) Geospat Health. 2020 Dec 29;15(2):. (PMID: 33461276) N Engl J Med. 2020 Jul 23;383(4):396-397. (PMID: 32699455) Bull World Health Organ. 2020 Mar 1;98(3):198-205. (PMID: 32132754) N Engl J Med. 2007 Mar 22;356(12):1277-8. (PMID: 17377173) Int J Infect Dis. 2020 Sep;98:401-405. (PMID: 32619763) PLoS One. 2020 Oct 22;15(10):e0240582. (PMID: 33091025) |
المشرفين على المادة: | 0 (DNA, Mitochondrial) 0 (RNA, Helminth) |
تواريخ الأحداث: | Date Created: 20211210 Date Completed: 20220218 Latest Revision: 20220218 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8694474 |
DOI: | 10.1371/journal.pntd.0009787 |
PMID: | 34890398 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1935-2735 |
---|---|
DOI: | 10.1371/journal.pntd.0009787 |